Abstract: A programmable receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality of native or endogenously expressed receptor subunits, wherein the native or endogenously expressed receptor subunits have been engineered or modified to include Fc?RI receptor components, biotin-binding components, or both, and wherein the Fc?RI receptor components and biotin-binding components are operative to bind to target detector molecules that bind to or otherwise interact with predetermined targets.

Abstract: A bioassay for the detection of multiple target analytes that includes living immunocytes of a plurality of predetermined types; a signal-generating reporter within each immunocyte that is responsive to predetermined changes occurring within the immunocyte and that includes a bioluminescent protein linked to a fluorescent protein; a signal transduction pathway associated with each signal-generating reporter that is operative to induce the predetermined changes within each immunocyte; a detector element associated with the signal transduction pathway that is operative to trigger the signal transduction pathway; an analyte binding element associated with the detector element that is specific to both the detector element and a target analyte; and wherein upon the binding of an analyte binding element to which a target analyte is also bound to the detector element, the detector element triggers the signal transduction pathway causing the predetermined changes to occur within the living biological cell, thereby ca

Abstract: A bioassay for the detection of multiple target analytes that includes living immunocytes of a plurality of predetermined types; a signal-generating reporter within each immunocyte that is responsive to predetermined changes occurring within the immunocyte and that includes a bioluminescent protein linked to a fluorescent protein; a signal transduction pathway associated with each signal-generating reporter that is operative to induce the predetermined changes within each immunocyte; a detector element associated with the signal transduction pathway that is operative to trigger the signal transduction pathway; an analyte binding element associated with the detector element that is specific to both the detector element and a target analyte; and wherein upon the binding of an analyte binding element to which a target analyte is also bound to the detector element, the detector element triggers the signal transduction pathway causing the predetermined changes to occur within the living biological cell, thereby ca

Abstract: A programmable receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality of native or endogenously-expressed receptor subunits, wherein at least one of the plurality of native or endogenously-expressed receptor subunits has been engineered or modified to include an Fc?RI receptor component or a biotin-binding component, and wherein the Fc?RI receptor component or biotin-binding component is operative to bind to a target detector molecule that binds to or otherwise interacts with a predetermined target.