Abstract: The methods, compositions and systems provided herein use functionalized beads to detect a target microparticle in a test sample, the method comprising (a) contacting the test sample containing the target microparticle with a plurality of functionalized beads. The size of the target microparticle is less than 1 ?m. The functionalized beads comprise beads coated with molecules of a capture agent, and at least some molecules of the capture agent bind to the target microparticle, thereby forming target microparticle-loaded beads comprising the functionalized beads and the target microparticle. The method further comprises (b) detecting the target microparticle-loaded beads using a flow cytometer, thereby detecting the presence of the target microparticle in the test sample, and the target microparticle-loaded the beads are detected with the detection limit that ranges from 10 microparticles per ml to 10e4 microparticles per ml.

Abstract: Disclosed is a graphical user interface to quickly build a graphical representation defining the set of instructions in a protocol without the user needing the programming knowledge to encapsulate those instructions in executable code. The graphical representation may include an arrangement of one or more graphical elements, with each graphical element corresponding to instructions or program logic. The user may also specify the set of parameters associated with each of the graphical elements. The arrangement of the one or more graphical elements, along with the set of parameters for each of the graphical elements, may be used to translate the graphical representation of the protocol into executable code for the protocol. The executable code for the protocol may then be executed by various flow cytometry machines in order to perform the protocol.

Abstract: Light from a light source is directed at a flow path of particles of a flow cytometer. The directed light results in a light pattern having a plurality of lobes. A first signal is detected exceeding a first threshold. A second signal exceeding a second threshold is detected, wherein the second threshold is greater than the first threshold. Based on detecting the second trigger after detecting the first trigger, is determined that the first and second signals were created by a relatively large particle.

Abstract: Disclosed is a graphical user interface to quickly build a graphical representation defining the set of instructions in a protocol without the user needing the programming knowledge to encapsulate those instructions in executable code. The graphical representation may include an arrangement of one or more graphical elements, with each graphical element corresponding to instructions or program logic. The user may also specify the set of parameters associated with each of the graphical elements. The arrangement of the one or more graphical elements, along with the set of parameters for each of the graphical elements, may be used to translate the graphical representation of the protocol into executable code for the protocol. The executable code for the protocol may then be executed by various flow cytometry machines in order to perform the protocol.

Abstract: Disclosed is a graphical user interface to quickly build a graphical representation defining the set of instructions in a protocol without the user needing the programming knowledge to encapsulate those instructions in executable code. The graphical representation may include an arrangement of one or more graphical elements, with each graphical element corresponding to instructions or program logic. The user may also specify the set of parameters associated with each of the graphical elements. The arrangement of the one or more graphical elements, along with the set of parameters for each of the graphical elements, may be used to translate the graphical representation of the protocol into executable code for the protocol. The executable code for the protocol may then be executed by various flow cytometry machines in order to perform the protocol.

Abstract: Disclosed is a graphical user interface to quickly build a graphical representation defining the set of instructions in a protocol without the user needing the programming knowledge to encapsulate those instructions in executable code. The graphical representation may include an arrangement of one or more graphical elements, with each graphical element corresponding to instructions or program logic. The user may also specify the set of parameters associated with each of the graphical elements. The arrangement of the one or more graphical elements, along with the set of parameters for each of the graphical elements, may be used to translate the graphical representation of the protocol into executable code for the protocol. The executable code for the protocol may then be executed by various flow cytometry machines in order to perform the protocol.

Abstract: A cytometry system having a computing system. The system includes a plurality of lasers controlled by the computing system to emit laser light. Each laser is spatially separated along a flow stream path. A detector system configured to receive light pulses from the plurality of lasers. The detector system being coupled to sampling circuitry. The computing system is configured to operate each of the plurality of lasers to independently emit laser light with respect to one another according to time of flight intervals along the flow stream path.

Abstract: Light from a light source is directed at a flow path of particles of a flow cytometer. The directed light results in a light pattern having a plurality of lobes. A first signal is detected exceeding a first threshold. A second signal exceeding a second threshold is detected, wherein the second threshold is greater than the first threshold. Based on detecting the second trigger after detecting the first trigger, is determined that the first and second signals were created by a relatively large particle.

Abstract: Light from a light source is directed at a flow path of particles of a flow cytometer. The directed light results in a light pattern having a plurality of lobes. A first signal is detected exceeding a first threshold. A second signal exceeding a second threshold is detected, wherein the second threshold is greater than the first threshold. Based on detecting the second trigger after detecting the first trigger, is determined that the first and second signals were created by a relatively large particle.

Abstract: A cytometry system having a computing system. The system includes a plurality of lasers controlled by the computing system to emit laser light. Each laser is spatially separated along a flow stream path. A detector system configured to receive light pulses from the plurality of lasers. The detector system being coupled to sampling circuitry. The computing system is configured to operate each of the plurality of lasers to independently emit laser light with respect to one another according to time of flight intervals along the flow stream path.

Abstract: Fluid is flowed into an inlet channel of a microfluidic pathway at a flow rate, the microfluidic pathway including a first and second outlet channels fluidically connected to the inlet channel. A first cavitation bubble is created within the first outlet channel to block fluid flow out of the first outlet channel. A second cavitation bubble is created within the second outlet channel to block fluid flow out of the second outlet channel. Creation of the second cavitation bubble is initiated during or after the first cavitation bubble dissolves such that the flow rate is maintained.

Abstract: Systems and methods for regarding a cytometry system are disclosed. The system can include a plurality lasers which are spatially separated from each other. Each laser can be assigned to a single detector. The single detector can process multiple events from each laser by digital switching of signal processing circuitry. Additional detectors can be assigned to receive light in a similar manner.

Abstract: A system and method for obtaining hydrodynamic focusing of a first fluid. The method includes pressurizing the first fluid and a second fluid at a pre-defined pressure from a pressure source. Further, the method includes controlling the first flow rate of the first fluid by passing it through a first flow circuit. Furthermore, the method includes controlling the second flow rate of the second fluid by passing it through a second flow circuit. Moreover, the method includes passing the first and the second fluid though a converging section and hydrodynamically focusing the first fluid.

Abstract: A system for pressurizing fluids is provided. The system includes a container with an open end and a closed end. The container contains the fluids to be pressurized. The system further includes a dynamic seal capable of expanding to facilitate sealing of the open end of the container. The system also includes a tube arrangement passing through the dynamic seal into the container. The tube arrangement facilitates passage of a first pressurizing medium to pressurize the fluids.

Abstract: Systems and methods for regarding a cytometry system are disclosed. The system can include a plurality lasers which are spatially separated from each other. Each laser can be assigned to a single detector. The single detector can process multiple events from each laser by digital switching of signal processing circuitry. Additional detectors can be assigned to receive light in a similar manner.

Abstract: The architecture of air-coupled laser sources in a flow cytometer is provided. The flow cytometer includes a mounting plate with a first major surface and a second major surface. A cuvette is mounted on the first major surface and a fluid core stream flows through the cuvette essentially parallel to the major surfaces of the mounting plate. A first air-coupled laser source is mounted on the first major surface. The first air-coupled laser source generates a first laser beam. The flow cytometer also includes a first beam-shaping optic system corresponding to the first air-coupled laser source. The first beam-shaping optic system receives the first laser beam and focuses it at the center of the fluid core stream perpendicular to the fluid core stream.

Abstract: A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.

Abstract: A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.

Abstract: A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.

Abstract: A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.