Abstract: A sensing device and method in detecting binding energy and binding kinetics between molecules; the sensing device has a sensor, a microfluidic chip and a measurement circuit. The sensor has multiple field effect transistors, and each field effect transistor adopts single-layer single crystal graphene as a conductive channel, thereby having extremely high sensitivity and stability; and the field effect transistors are arranged in an array and are provided with multichannel measurement circuits to detect the binding dynamics processes of different molecules or different copies of the same molecule in parallel so as to meet the high-throughput detection requirements. A compound that is non-covalently bound with the single-layer single crystal graphene as a medium to fix probe molecules on the surface of the conductive channel, thereby retaining the intrinsic structure of the graphene, and improving the signal-to-noise ratio and the sensitivity of the graphene field effect transistor.