Abstract: While green algae are expected to serve as raw materials of biomass fuels, they are damaged by high-intensity light when subjected to mass-culture outdoors in summer, and biomass productivity is deteriorated as a consequence. In order to overcome such a drawback, the present invention provides a high-intensity light resistant green algae mutant that can be subjected to outdoor culture in summer. Specifically, the present invention relates to such green algae mutant, wherein functions or expression levels of a protein having a response regulatory domain at the N-terminus and a WD40 domain at the C-terminus are lower than those in a wild-type strain, and wherein said green algae mutant grows faster than a wild-type strain when cultured at a light intensity of 1,000, 1,500, or 2,000 ?mol photons m?2 s?1 measured as photosynthetically active radiation (PAR).

Abstract: It is an object of the present invention to provide the eukaryotic microalgae, which have been genetically modified such that larger amounts of assimilation products produced by photosynthesis are directed to the synthesis of triglyceride (=triacylglycerol; TAG), and specifically, the present invention relates to a genetically modified strain of eukaryotic microalgae, in which a gene encoding an AGL1 protein is highly expressed, or a gene encoding an FAT1 protein and/or a gene encoding a DGAT2 protein are further highly expressed, as well as the gene encoding an AGL1 protein, wherein TAG productivity is improved in comparison to the parent strain thereof.

Abstract: While green algae are expected to serve as raw materials of biomass fuels, they are damaged by high-intensity light when subjected to mass-culture outdoors in summer, and biomass productivity is deteriorated as a consequence. In order to overcome such a drawback, the present invention provides a high-intensity light resistant green algae mutant that can be subjected to outdoor culture in summer. Specifically, the present invention relates to such green algae mutant, wherein functions or expression levels of a protein having a response regulatory domain at the N-terminus and a WD40 domain at the C-terminus are lower than those in a wild-type strain, and wherein said green algae mutant grows faster than a wild-type strain when cultured at a light intensity of 1,000, 1,500, or 2,000 ?mol photons m?2 s?1 measured as photosynthetically active radiation (PAR).

Abstract: A green alga variant having a dual-specificity tyrosine-phosphorylation regulated protein kinase activity that is reduced compared to a dual-specificity tyrosine-phosphorylation regulated protein kinase activity of a parental strain is provided. The green alga variant increases a total amount of a lipid production per unit time and per unit culture area compared to a total amount of a lipid production of the parental strain. A dual-specificity tyrosine-phosphorylation regulated protein kinase of the parental strain is a protein having an amino acid sequence with at least 50% sequence identity with the amino acid sequence of an active site and a substrate recognition site of SEQ ID NO: 4 and having the dual-specificity tyrosine-phosphorylation regulated protein kinase activity.

Abstract: It is an object of the present invention to provide the eukaryotic microalgae, which have been genetically modified such that larger amounts of assimilation products produced by photosynthesis are directed to the synthesis oftriglyceride (=triacylglycerol; TAG), and specifically, the present invention relates to a genetically modified strain of eukaryotic microalgae, in which a gene encoding an AGL1 protein is highly expressed, or a gene encoding an FAT1 protein and/or a gene encoding a DGAT2 protein are further highly expressed, as well as the gene encoding an AGL1 protein, wherein TAG productivity is improved in comparison to the parent strain thereof.

Abstract: A culture method for microalgae that cultures unicellular green microalgae belonging to the genus Coccomyxa and groups of organisms closely related thereto, or the Watanabea clade, in an open outdoor culture system using broth having a pH of 4 or lower. A culture method for microalgae that cultures microalgae of the genus Coccomyxa and groups of organisms closely related thereto, or Pseudococcomyxa, in an open outdoor culture system using broth having a pH of 4 or lower containing ammonia nitrogen.

Abstract: A green alga variant having a dual-specificity tyrosine-phosphorylation regulated protein kinase activity that is reduced compared to a dual-specificity tyrosine-phosphorylation regulated protein kinase activity of a parental strain is provided. The green alga variant increases a total amount of a lipid production per unit time and per unit culture area compared to a total amount of a lipid production of the parental strain. A dual-specificity tyrosine-phosphorylation regulated protein kinase of the parental strain is a protein having an amino acid sequence with at least 50% sequence identity with the amino acid sequence of an active site and a substrate recognition site of SEQ ID NO: 4 and having the dual-specificity tyrosine-phosphorylation regulated protein kinase activity.

Abstract: A culture method for microalgae that cultures unicellular green microalgae belonging to the genus Coccomyxa and groups of organisms closely related thereto, or the Watanabea clade, in an open outdoor culture system using broth having a pH of 4 or lower. A culture method for microalgae that cultures microalgae of the genus Coccomyxa and groups of organisms closely related thereto, or Pseudococcomyxa, in an open outdoor culture system using broth having a pH of 4 or lower containing ammonia nitrogen.

Abstract: A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).

Abstract: A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).

Abstract: This invention relates to S-hydroxynitrile lyase having excellent tolerance to heat, organic solvents, and the like, which is obtained by modifying at least one amino acid in the helix D3, helix A, and ?-sheet 2 domains in the amino acid sequence of wild-type S-hydroxynitrile lyase.

Abstract: A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).

Abstract: This invention relates to S-hydroxynitrile lyase having excellent tolerance to heat, organic solvents, and the like, which is obtained by modifying at least one amino acid in the helix D3?, helix A, and ?-sheet 2 domains in the amino acid sequence of wild-type S-hydroxynitrile lyase.

Abstract: A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).

Abstract: A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).

Abstract: A process using microorganisms which contain genes, which form an active xylene monooxygenase, which form no effective, chromosomally or plasmid-coded alcohol hydrogenase, and which are, thus, capable of hydroxylating methyl groups on aromatic 5- or 6-atom heterocycles to the corresponding hydroxymethyl derivatives, for the production of hydroxymethylated 5- or 6-atom heterocycles.