Patents by Inventor Shigefumi Kumachi

Shigefumi Kumachi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240002838
    Abstract: Provided is a linker for both screening assessment of the candidate clones without using enzymes, and to provide an in vitro selection method using thereof. Also, provided is a high-speed photo-crosslinking linker for molecular interaction analysis and in vitro selection comprising a backbone and a side chain. The backbone comprises a solid-phase binding site located at the 5? terminus for forming a bond with a solid-phase; a solid-phase cleavage site for releasing the entire solid-phase at the site; a side chain linking site for linking a side chain; a high-speed photo-crosslinking site for linking the backbone to mRNA having a sequence complementary thereof via photo-crosslinking; and a reverse transcription initiation region located adjacent to the side chain linking site at the 3? terminus of the backbone. The side chain comprises a fluorescent label, a protein binding site located at the free terminus thereof; and a binding site with the backbone.
    Type: Application
    Filed: September 15, 2023
    Publication date: January 4, 2024
    Applicant: Saitama University
    Inventors: Naoto Nemoto, Yuki Mochizuki, Shigefumi Kumachi
  • Publication number: 20230365659
    Abstract: A peptide which binds to SARS-CoV-2 and its usage are provided. The peptide which binds to SARS-CoV-2 comprises one or more structural domain comprising CDR3 consisting of an amino acid sequence of any of SEQ ID NOs: 1 to 9 or an amino acid sequence obtained by substituting at least one amino acid in the amino acid sequence with another amino acid.
    Type: Application
    Filed: October 1, 2021
    Publication date: November 16, 2023
    Applicants: EPSILON MOLECULAR ENGINEERING, INC., THE KITASATO INSTITUTE, Kao Corporation
    Inventors: Hidekazu Masaki, Shigefumi KUMACHI, Ryo YONEHARA, Yuta MATSUMURA, Hibiki KAWANO, Takuto TOJO, Takuya MORIMOTO, Kazunhiko KATAYAMA, Kei HAGA, Reiko TODAKA, Akihito SAWADA, Tomomi TAKANO
  • Publication number: 20220229058
    Abstract: Provided is a peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 185.
    Type: Application
    Filed: May 28, 2020
    Publication date: July 21, 2022
    Applicant: Kao Corporation
    Inventors: Yoshitaka ITO, Takuya MORIMOTO, Shigefumi KUMACHI, Naoto NEMOTO
  • Publication number: 20220227809
    Abstract: A peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus is provided. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 327.
    Type: Application
    Filed: May 28, 2020
    Publication date: July 21, 2022
    Applicant: Kao Corporation
    Inventors: Yoshitaka ITO, Takuya MORIMOTO, Shigefumi KUMACHI, Naoto NEMOTO
  • Publication number: 20220221458
    Abstract: A peptide that specifically binds to norovirus, which is useful for detection and infection control of norovirus is provided. A norovirus-binding peptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 269.
    Type: Application
    Filed: May 28, 2020
    Publication date: July 14, 2022
    Applicant: Kao Corporation
    Inventors: Yoshitaka ITO, Takuya MORIMOTO, Shigefumi KUMACHI, Naoto NEMOTO
  • Publication number: 20210381026
    Abstract: The present invention provides an improved immuno-PCR method by using cDNA display comprising the steps of: immobilizing a first antibody having a binding site to a solid phase: contacting a sample fluid to said antibody to bind a target molecule in said sample fluid; contacting said target molecule to a cDNA display being composed of a backbone being composed of a double strand and a side chain having a second antigen binding site to which the second antibody is bound; and conducting polymerase chain reaction to detect said cDNA quantitatively. According to the improved immune-PCR method of the present invention, the target molecule is screened and obtained quantitatively, because it uses cDNA display being composed of one protein/peptide and one DNA.
    Type: Application
    Filed: August 21, 2021
    Publication date: December 9, 2021
    Applicants: EPSILON MOLECULAR ENGINEERING INC., Saitama University
    Inventors: Naoto Nemoto, Hironori Anzai, Takeru Suzuki, Shigefumi Kumachi, Takuya Terai
  • Publication number: 20180100146
    Abstract: Provided is a linker for both screening assessment of the candidate clones without using enzymes, and to provide an in vitro selection method using thereof. Also, provided is a high-speed photo-crosslinking linker for molecular interaction analysis and in vitro selection comprising a backbone and a side chain. The backbone comprises a solid-phase binding site located at the 5? terminus for forming a bond with a solid-phase; a solid-phase cleavage site for releasing the entire solid-phase at the site; a side chain linking site for linking a side chain; a high-speed photo-crosslinking site for linking the backbone to mRNA having a sequence complementary thereof via photo-cros slinking; and a reverse transcription initiation region located adjacent to the side chain linking site at the 3? terminus of the backbone. The side chain comprises a fluorescent label, a protein binding site located at the free terminus thereof; and a binding site with the backbone.
    Type: Application
    Filed: September 29, 2017
    Publication date: April 12, 2018
    Inventors: Naoto Nemoto, Yuki Mochizuki, Shigefumi Kumachi