Patents by Inventor Shigeki Otawara

Shigeki Otawara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for producing tissue plasminogen activator
    Patent number: 4935368
    Abstract: This invention discloses a process for producing tissue plasminogen activator. The process comprises cultivating Escherichia coli, yeast, or mammalian cells into which the vector having the gene for human tissue plasminogen activator (tPA) has been introduced by the recombinant DNA technology. In the cultivation or purification process, a specific antiplasmin agent, which is one of the protease inhibitors, is added to the medium and/or the buffer solutions used for purification, so as to prevent the conversion of single-chain form tPA into double-chain form tPA by a protease which is present in the medium.
    Type: Grant
    Filed: June 27, 1986
    Date of Patent: June 19, 1990
    Assignees: Toyo Boseki Kabushiki Kaisha, Daiichi Seiyaku Co., Ltd.
    Inventors: Ryotaro Kotani, Tsuneo Unuma, Shigeki Otawara, Setsuo Kobayashi, Tadao Suzuki