Abstract: It is intended to provide by improving a known method of producing hyaluronic acid in a plant, a plant or a cultured plant cells which can produce hyaluronic acid at a lower cost and a further higher yield than before, a method of preparing the same, an expression vector for transformation, a method of producing hyaluronic acid using the plant or the cultured plant cells and the like. The method of producing hyaluronic acid comprising obtaining hyaluronic acid by co-expressing a protein with hyaluronic acid synthase activity and an exogenous protein with sugar-nucleotide synthase activity in a plant cell or a plant is provided.

Abstract: It is intended to provide by improving a known method of producing hyaluronic acid in a plant, a plant or a cultured plant cells which can produce hyaluronic acid at a lower cost and a further higher yield than before, a method of preparing the same, an expression vector for transformation, a method of producing hyaluronic acid using the plant or the cultured plant cells and the like. The method of producing hyaluronic acid comprising obtaining hyaluronic acid by co-expressing a protein with hyaluronic acid synthase activity and an exogenous protein with sugar-nucleotide synthase activity in a plant cell or a plant is provided.

Abstract: The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.

Abstract: The invention relates to a method of producing hyaluronic acid comprising (1) a step of transforming a plant cell using an expression recombinant vector comprising (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.

Abstract: Disclosed are a polymerizable sugar ester of the formula shown below and a process for the production thereof:S(OH).sub.q-1 --OOC--R--COOCH.dbd.CH.sub.2wherein S(OH).sub.q-1, represents a sugar residue obtained by removing one hydroxyl group from a sugar compound composed of a sugar skeleton S and q number of hydroxyl groups bonded thereto, and R represents an alkylene group. The polymerizable sugar ester is produced by reacting a sugar compound represented by the following general formula:S(OH).sub.qwherein S represents the sugar skeleton and q is the number of the hydroxyl groups bonded thereto, with a divinyl ester of an aliphatic dicarboxylic acid represented by the following general formula:CH.sub.2 .dbd.CHOOC--R--COOCH.dbd.CH.sub.2in the presence of a hydrolytic enzyme. The polymerizable sugar ester gives a sugar-based polymer by polymerization.