Abstract: A method and apparatus for detecting presence of liquid in a pathway of known volume feature a molding with a sample receiving port, a pump connection port, at least one sample treating chamber and optionally at least one optical measuring chamber located between the sample receiving port and the pump connection port. The sample receiving port and the treating chambers are connected with each other through a pathway, which passes through the liquid detecting part, that has at least one air hole that is controlled to be in an open or closed state in response to the presence of liquid detected by the liquid detecting part. A sample is quantitatively fed from the sample receiving port into a sample treating chamber. Detection of the liquid involves projecting a light beam at an incident angle in a range of 42.degree. to 62.degree.

Abstract: By using an assay device comprising a sample-receiving port, a pump-connection port, a sample-treating zone which is provided with a labeled substance with a label, a sample-treating and optical-measuring zone which has a porous material having immobilized thereto one of a specifically binding pair, and a pathway interconnecting these zones and ports, wherein the sample-treating zone and the sample-treating and optical-measuring zone are positioned between the sample-receiving port and the pump-connection port;or by using an assay device comprising a sample-receiving port, a pump-connection port, a sample-treating and optical-measuring zone which has a porous material having immobilized thereto a labeled substance with a label and one of a specifically binding pair, and a pathway interconnecting these zone and ports, wherein the sample-treating and optical-measuring zone is positioned between the sample-receiving port and the pump-connection port,biological components can be quantitatively determined by immun

Abstract: In a method of measuring optical characteristics of a component in a liquid impregnated in a porous material with no affection by the porous material, a light beam having an absorbable wavelength which is absorbed by the component in the liquid and a light beam having a wavelength which is longer than the absorbable wavelength are projected to the porous material, and a quantity of light having the wavelength which is absorbed by the component in the liquid is compensated by a quantity of transmitted light having the wavelength longer than the absorbable wavelength.

Abstract: The biological fluid analyzing device for analyzing biological fluid by measuring optical characteristics of a sample has a sample receiving port 1 and a pump connection port 5. Between the sample receiving port 1 and the pump connection port 5, the analyzing device has either a combination of at least one sample-treating chamber 2a, 2b, 2c and at least one optical-measuring chamber 3a, 3b, or a combination of at least one sample-treating chamber, at least one optical-measuring chamber and at least one waste liquid reservoir 4. The sample receiving port 1 is connected with one sample-treating chamber; the pump connection port 5 is connected with a pathway 6 or waste liquid reservoir 4; and the sample-treating chamber and optical-measuring chamber are interconnected with the pathway, or the sample-treating chamber, optical-measuring chamber and waste liquid reservoir are interconnected with the pathway.

Abstract: Disclosed is an analysis implement including a support and a reagent layer containing an oxidase applied to the support such that one surface of the reagent layer is exposed to an air atmosphere. A hydrophobic, porous, oxygen permeable layer is positioned between the reagent layer and the support, such that oxygen can permeate to the layer containing oxidase.