Abstract: The present invention is intended to develop a chimeric antigen receptor (CAR) that is effective against solid tumor expressing anaplastic lymphoma kinase (ALK). The present invention provides a polynucleotide encoding a CAR protein comprising a target binding domain binding to an extracellular ligand binding region of ALK, a transmembrane domain, and an intracellular signaling domain. The target binding domain of the polynucleotide is selected from among FAM150A, FAM150B, and fragments thereof binding to the extracellular ligand binding region of ALK. The present invention also provides a genetically modified cell comprising the polynucleotide introduced thereinto.

Abstract: It is intended to produce a cell expressing a mutant chimeric antigen receptor (CAR) having excellent cytotoxicity to target cells. The present invention provides a genetically modified cell having introduced thereinto a polynucleotide encoding a chimeric antigen receptor (CAR) protein having a target binding domain that specifically binds to a human granulocyte-macrophage colony stimulating factor (GM-CSF) receptor, a transmembrane domain, and an intracellular signaling domain, wherein the tar get binding domain is a mutant having the substitution of glutamic acid at position 21 in the amino acid sequence shown in SEQ ID NO: 1 with another amino acid.

Abstract: Production of a chimeric antigen receptor (CAR) expressing cell having excellent target cytotoxicity. Provided is a genetically modified cell comprising, introduced thereinto, a polynucleotide encoding a chimeric antigen receptor (CAR) protein having a target binding domain that specifically binds to a human granulocyte-macrophage colony stimulating factor (GM-CSF) receptor, a transmembrane domain and an intracellular signaling domain.

Abstract: A packaged article includes a packing bag which includes a welded portion that hermetically seals a container compartment in which a fluid content is contained. The welded portion includes a weak sealant section configured to be parted when the container compartment is pressed to communicate the container compartment and the exterior of the packing bag. A strong sealant section is configured to maintain a welded state at the time when the weak sealant section is parted. The container compartment includes a primary container section and a secondary container section. The secondary container section includes a narrow neck that is narrower than the primary container section in a width-wise direction, which corresponds to a direction in which the weak sealant section extends. The secondary container section is in communication with the primary container section at the narrow neck. The secondary container section is located adjacent to the weak sealant section.

Abstract: Production of a chimeric antigen receptor (CAR) expressing cell having excellent target cytotoxicity. Provided is a genetically modified cell comprising, introduced thereinto, a polynucleotide encoding a chimeric antigen receptor (CAR) protein having a target binding domain that specifically binds to a human granulocyte-macrophage colony stimulating factor (GM-CSF) receptor, a transmembrane domain and an intracellular signaling domain.

Abstract: A packaged article includes a packing bag which includes a welded portion that hermetically seals a container compartment in which a fluid content is contained. The welded portion includes a weak sealant section configured to be parted when the container compartment is pressed to communicate the container compartment and the exterior of the packing bag. A strong sealant section is configured to maintain a welded state at the time when the weak sealant section is parted. The container compartment includes a primary container section and a secondary container section. The secondary container section includes a narrow neck that is narrower than the primary container section in a width-wise direction, which corresponds to a direction in which the weak sealant section extends. The secondary container section is in communication with the primary container section at the narrow neck. The secondary container section is located adjacent to the weak sealant section.

Abstract: The present invention relates to a method for producing a high-acidity vinegar with improved efficiency compared with conventional methods and a high-acidity vinegar obtained by such method. The method of the present invention is characterized in that acetic acid fermentation is conducted with a culture solution which contains a compound having a thiol group or an S-substituted derivative thereof or a compound having a disulfide bond as an additive wherein a total nitrogen content of the culture solution is 0.023 w/v % or less. According to the method of the present invention, a high-acidity vinegar, which has a total nitrogen content of 0.015 w/v % or less and an acidity of 10 to 25 w/v % and contains a compound having a thiol group or an S-substituted derivative thereof or a compound having a disulfide bond at a concentration of 17 ?M or more in terms of sulfur atoms contained in the thiol group or disulfide bond can be obtained.

Abstract: According to one embodiment, a flexographic printing plate material includes a printing layer for engraving containing rubber, a compressive layer, a base fabric layer provided between the printing layer for engraving and the compressive layer, and a reinforcement layer.

Abstract: According to one embodiment, a flexographic printing plate material includes a printing layer for engraving containing rubber, a compressive layer, a base fabric layer provided between the printing layer for engraving and the compressive layer and a reinforcement layer. The plate material has a thickness of more than 2.75 mm and less than or equal to 7 mm. A ratio of a thickness of the printing layer for engraving to the thickness of the plate material is greater than or equal to 10% and less than or equal to 78%, and a ratio of a thickness of the compressive layer to the thickness of the plate material is greater than or equal to 6% and less than or equal to 78%.

Abstract: The present invention relates to a method for producing a high-acidity vinegar with improved efficiency compared with conventional methods and a high-acidity vinegar obtained by such method. The method of the present invention is characterized in that acetic acid fermentation is conducted with a culture solution which contains a compound having a thiol group or an S-substituted derivative thereof or a compound having a disulfide bond as an additive wherein a total nitrogen content of the culture solution is 0.023 w/v % or less. According to the method of the present invention, a high-acidity vinegar, which has a total nitrogen content of 0.015 w/v % or less and an acidity of 10 to 25 w/v % and contains a compound having a thiol group or an S-substituted derivative thereof or a compound having a disulfide bond at a concentration of 17 ?M or more in terms of sulfur atoms contained in the thiol group or disulfide bond can be obtained.

Abstract: The invention provides a gas fuel supply kit for the existing vehicle (vehicle mounting a gasoline engine, or a diesel engine), in order to use a gas fuel such as hydrogen after wards. A post-installable type gas fuel supply kit to be installed in the existing vehicle such as a gasoline vehicle, comprising a gas fuel injector for supplying the gas fuel such as hydrogen to an intake path; an injector installing means for installing said gas fuel injector to a location which is a different location where a gasoline injector is installed; and a control unit for controlling at least an injection amount, and an injection timing of the gas fuel at the hydrogen fuel injector.

Abstract: Provided is a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut. The method involves a PCR method for specifically detecting nuts of the family Juglandaceae and identifying the matK sequence of pecan nut to thereby discriminate walnut and pecan nut from each other optionally using restriction enzymes.

Abstract: [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut. [Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.

Abstract: The present invention provides a gene involved in the growth-promoting function of acetic acid bacteria and a microorganism containing the gene. In particular, the present invention provides a method for enhancing the growth-promoting function of acetic acid bacteria and a method for efficiently producing vinegar containing acetic acid at a high concentration in a short time using such acetic acid bacteria having an enhanced growth-promoting function.

Abstract: The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid.

Abstract: It is intended to provide a method of conferring to microbial cells or the like, tolerance to a short chain fatty acid, including formic acid and acetic acid, which is harmful to the growth thereof without suppressing the growth of the cells while maintaining a high productivity of useful substances. It is also intended to provide a means for efficiently culturing a microorganism in the presence of a short chain fatty acid, or a means for producing a useful short chain fatty acid via fermentation.

Abstract: A novel gene having a function of improving acetic acid fermentation in practical level was cloned from a practical acetic acid bacterium belonging to the genus Gluconacetobacter by a method for obtaining a gene having a growth-promoting function in acetic acid-containing medium from the chromosomal DNA library of the acetic acid bacterium. It was made possible to significantly shorten the growth induction period and significantly improve the acetic acid fermentation rate of transformants obtained by introducing the gene into acetic acid bacteria, when such transformants are cultured in the presence of ethanol.

Abstract: The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

Abstract: A method for preventing or treating metabolic syndrome by administering bezafibrate. Since bezafibrate suppresses the action of 11?-hydroxysteroid dehydrogenase type 1 and also accelerates expression of adiponectin receptor, it is used as an agent for preventing or treating metabolic syndrome.

Abstract: A novel gene having a function of improving acetic acid fermentation in practical level was cloned from a practical acetic acid bacterium belonging to the genus Gluconacetobacter by a method for obtaining a gene having a growth-promoting function in acetic acid-containing medium from the chromosomal DNA library of the acetic acid bacterium. It was made possible to significantly shorten the growth induction period and significantly improve the acetic acid fermentation rate of transformants obtained by introducing the gene into acetic acid bacteria, when such transformants are cultured in the presence of ethanol.