Abstract: Increased yields of the cellulolytic enzymes from Thielavia terrestris are effected by novel separatory methods for the produced enzymes as well as the enhancement of the production of one of the enzymes, namely .beta.-glucosidase, by the addition of glycerol to the media. Further, since the .beta.-glucosidase is responsible for glucose production from cellulose, a method is provided wherein the separated .beta.-glucosidase can be employed as, for example, on a fixed support in a position to receive concentrated streams of partially converted cellulosic materials, thereby leading to enhanced glucose production.