Abstract: The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 ?L of NaOH-DMSO suspension (1 mg/mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 ?L of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

Abstract: A method for promoting the solubility of rhamnogalacturonan I (RG-I) pectin based on polysaccharide interaction is provided. The method includes the following steps: adding an aqueous solution of negatively charged polysaccharides to an RG-I pectin extract, mixing uniformly, adjusting the potential of Hydrogen (pH) of the compound to 3-7, performing ethanol precipitation to obtain a precipitate; washing the precipitate, followed by drying and crushing to obtain a polysaccharide-pectin compound; where the RG-I pectin extract is obtained by extracting orange peel powder with dilute hydrochloric acid.

Abstract: The present disclosure provides a method for manufacturing a cooked grilling sausage. In the present disclosure, lean animal meat is subjected to a high-voltage pulsed electric field treatment to promote dissolution of salt-soluble proteins in the lean animal meat and to promote hydration of the salt-soluble proteins during curing. This process helps a resulting minced meat form a three-dimensional gel-like texture, thus making the cooked grilling sausage chewy, tender, and juicy. A raw material of the cooked grilling sausage includes lean animal meat, animal fat, sucrose, salt, Chinese liquor, complex phosphates, and crushed ice. By using the complex phosphates with a low viscosity instead of a plant-derived texture restructuring gum, a hydrophilic colloid stabilizer, and a water-retaining agent, a texture of the fresh lean animal meat can be transformed into a tender, elastic, and juicy texture of the cooked grilling sausage with a high water-holding capacity.

Abstract: The present disclosure provides a method for manufacturing a cooked grilling sausage. In the present disclosure, lean animal meat is subjected to a high-voltage pulsed electric field treatment to promote dissolution of salt-soluble proteins in the lean animal meat and to promote hydration of the salt-soluble proteins during curing. This process helps a resulting minced meat form a three-dimensional gel-like texture, thus making the cooked grilling sausage chewy, tender, and juicy. A raw material of the cooked grilling sausage includes lean animal meat, animal fat, sucrose, salt, Chinese liquor, complex phosphates, and crushed ice. By using the complex phosphates with a low viscosity instead of a plant-derived texture restructuring gum, a hydrophilic colloid stabilizer, and a water-retaining agent, a texture of the fresh lean animal meat can be transformed into a tender, elastic, and juicy texture of the cooked grilling sausage with a high water-holding capacity.

Abstract: A 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid in the preparation of a plant immunity elicitor. 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid as a natural active substance is developed into a plant immunity elicitor. The plant immunity elicitor can be used for improving the resistance of plants to a biotic stress and an abiotic stress, effectively preventing fungi, bacteria and viruses from infecting the plants, and further reducing the pathogenicity. Meanwhile, the plant immunity elicitor can significantly improve resistant ability of the plants to high temperature, low temperature, drought and salt stresses. 2-amino-3-indolyl butyric acid or 3-methylpyrrolidine-2-carboxylic acid has the characteristics of safety, environmental protection and high efficiency.

Abstract: A method for extracting and identifying a polysaccharide from a complex sample. The method includes: subjecting a complex sample to be treated to sterilization and enzyme deactivation to obtain a system, and subjecting the system to solid-liquid separation to obtain a sterilization and enzyme deactivation-treated solution; deproteinizing the sterilization and enzyme deactivation-treated solution to obtain a deproteinized solution; purifying the deproteinized solution by using a hydrophilic lipophilic balance-solid phase extraction (HLB-SPE) cartridge to obtain a purified solution; subjecting the purified solution to alcohol precipitation to obtain the polysaccharide; and subjecting the polysaccharide to methylation, glycoside residue derivatization and liquid chromatography-mass spectrometry (LC-MS) analysis in sequence.

Abstract: A 2-amino-3-hydroxy-3-methylbutyric acid and/or 2-amino-3-(4-hydroxyphenyl) butyric acid are used as natural active substances to be developed as a plant immune resistance inducer which can be used for improving the resistance of plants to biotic stress and abiotic stress and effectively prevents infection of fungi, viruses and bacteria on plants and reduces pathogenic levels, moreover, the tolerance capability of plants to high-temperature, low-temperature, drought and salt stresses can be remarkably improved. The 2-amino-3-hydroxy-3-methylbutyric acid and 2-amino-3-(4-hydroxyphenyl) butyric acid have the characteristics of safety, environmental protection and high efficiency.

Abstract: The present disclosure provides a compound and methods of preparing the compound. The compound has anti-microbial and anti-mite properties and can be bonded to materials such as fiber and synthetic polymer. The compound may also be used in a finishing agent for textiles, fibers, or yarns to protect these materials against microorganisms and mites. Additionally, when these materials are treated with a relatively low dosage of the compound, long-lasting and effective anti-mold and anti-mite effects may be achieved at a relatively low cost.

Abstract: The use of 2-amino-3-phenylbutyric acid or 2,6-diamino-3-methylhexanoic acid as a plant immune resistance inducer. The development of 2-amino-3-phenylbutyric acid and/or 2,6-diamino-3-methylhexanoic acid as an active substance into a plant immune resistance inducer can be used to improve the resistance of plants to biotic and abiotic stresses, effectively prevent the infection and reduce the pathogenic levels of fungi, viruses, and bacteria on plants; Meanwhile, they can significantly improve the resistance of plants to high temperature, low temperature, drought and salt stresses. 2-amino-3-phenylbutyric acid and 2,6-diamino-3-methylhexanoic acid have the features of being safe, environmentally friendly and efficient.

Abstract: Disclosed are a metal-organic framework composite and a preparation method and use thereof. The metal-organic framework composite includes a cyclodextrin metal-organic framework (CD-MOF) material, and nano-silver and caffeic acid that are loaded in the CD-MOF material; wherein the nano-silver is loaded at 4% to 5% of a total mass of the composite; and caffeic acid is loaded at 11% to 12% of the total mass of the composite. The method for preparing the metal-organic framework composite includes: placing a CD-MOF material in an ethanol solution containing silver nitrate to obtain a first mixture, and subjecting the first mixture to a dynamic contact reaction in the dark to obtain a CD-MOF loaded with nano-silver; and placing the CD-MOF loaded with nano-silver in an ethanol solution containing caffeic acid to obtain a second mixture, and subjecting the second mixture to an incubation in the dark to obtain the composite.

Abstract: Provided are a Ganoderma lucidum compound preparation, and a preparation method and use thereof. The preparation method includes: mixing a superfine powder of a Ganoderma lucidumfruiting body, ?-cyclodextrin and a part of water to allow embedding to obtain a first feed liquid; mixing the first feed liquid with a composite colloid stabilizer solution to obtain a second feed liquid, a colloid stabilizer in the composite colloid stabilizer solution including at least three agar, guar gum, locust bean gum, carrageenan, and xanthan gum; mixing the second feed liquid with a metal ion solution to allow adjustment to acidic and shearing sequentially to obtain a third feed liquid, metal ions in the metal ion solution including iron ions, calcium ions, or zinc ions; and mixing the third feed liquid with remaining water, subjecting a resulting mixture to homogenization and heating sequentially to obtain the Ganoderma lucidum compound preparation.

Abstract: Provided are a food additive for dispersing a Ganoderma lucidum powder and a method for rapidly dispersing a Ganoderma lucidum powder. The food additive for dispersing a Ganoderma lucidum powder includes a plant polyphenol and a metal-derived food additive in a mass ratio of (50-2,500):(0.1-0.2). The method for rapidly dispersing a Ganoderma lucidum powder with the food additive includes: mixing an aqueous solution of the plant polyphenol with the Ganoderma lucidum powder to obtain a Ganoderma lucidum/polyphenol mixed solution; and thoroughly mixing an aqueous solution of the metal-derived food additive with the Ganoderma lucidum/polyphenol mixed solution.

Abstract: The present disclosure provides use of an intestinal probiotic in preparation of a medicament that promotes metabolism of ellagic acid (EA) into urolithin A, a pharmaceutical composition and use thereof, and belongs to the technical field of biomedicine. In the present disclosure, probiotics capable of changing metabotypes of EA are selected from a plurality of probiotics. These probiotics alone or in combination can change an intestinal flora structure of a metabotype B and/or 0 population, thereby improving the efficiency of the metabolism of EA into urolithin A and increasing a yield of urolithin A. The present disclosure provides an intestinal probiotic for involving in and promoting the metabolism of EA into urolithin A, and provides a new idea for the treatment of diabetes, obesity, senescence, and diseases caused by central nervous system lesions and viral infection using urolithin A as an active pharmaceutical ingredient.

Abstract: The present disclosure provides a water-soluble dietary fiber (WSDF), use thereof in preparation of a medicament that promotes metabolism of ellagic acid (EA) into urolithin A, and a pharmaceutical composition, and belongs to the technical field of biological pharmaceuticals. The WSDF provided by the present disclosure includes at least two components selected from the group consisting of gellan gum, guar gum, carrageenan, fructooligosaccharide, xylooligosaccharide, malto-oligosaccharide, pectin, ?-glucan, polydextrose, sodium alginate, gum arabic, resistant dextrin, xanthan gum, tragacanth gum, and inulin. In the present disclosure, compounding the foregoing different combinations of the WSDF and EA to feed mice may significantly increase the average urolithin A content in mouse feces.

Abstract: A use of 2-amino-3-methylhexanoic acid in promoting plant growth and increasing yield and use of the 2-amino-3-methylhexanoic acid in promoting growth of a plant seedling and/or promoting growth of a mature plant and increasing yield. The 2-amino-3-methylhexanoic acid is a natural product, has a simple structure, and is easy for industrial production. The 2-amino-3-methylhexanoic acid can promote plant growth and has the potential of being developed into a natural plant growth regulator. The 2-amino-3-methylhexanoic acid is a natural product, low in dosage, and environmentally friendly, has a remarkable promoting effect on plant growth, and thus is a green and efficient biological source plant growth regulator, which indicates the utilization value and application prospects of such substances in agricultural production.

Abstract: The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 ?L of NaOH-DMSO suspension (1 mg/mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 ?L of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

Abstract: The disclosure aims to provide a chemical synthesis method of a prodelphinidin B9 gallate, in which the prodelphinidin B9 gallate is synthesized by using a proanthocyanidin from Chinese bayberry leaves as a raw material, and using epigallocatechin gallate (EGCG) as a nucleophilic reagent to attack C4 sites of subunits EGCG and epigallocatechin (EGC) of the proanthocyanidin from Chinese bayberry leaves in the presence of hydrochloric acid as a catalyst. Compared with prodelphinidin B9 gallates extracted and separated from materials such as tea leaves, and Chinese bayberry leaves, the prodelphinidin B9 gallate prepared by the method provided in the present disclosure has higher purity and yield, and can be directly used as a nutrient enhancer and a natural antioxidant in the field of food.

Abstract: Disclosed are a caffeic acid-based composite material and a preparation method thereof. The method includes: exposing a cyclodextrin metal-organic framework material prepared from ?-cyclodextrin to a solution of caffeic acid in a short-chain alcohol to obtain a mixed material; and incubating the mixed material, wherein during the incubating, the cyclodextrin metal-organic framework material is in dynamic contact with the solution of caffeic acid in a short-chain alcohol. The prepared composite material includes a cyclodextrin metal-organic framework material and caffeic acid loaded on the cyclodextrin metal-organic framework material, wherein the cyclodextrin metal-organic framework material is prepared from ?-cyclodextrin. The caffeic acid is loaded in an amount of 15-18% of a total mass of the caffeic acid-based composite material. The caffeic acid is located in a cavity of the cyclodextrin metal-organic framework material.

Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.

Abstract: Disclosed is use of 2-amino-3-methylhexanoic acid as a plant immunity inducer and in the preparation of a plant immunity inducer. The development of 2-amino-3-methylhexanoic acid as an active substance into a plant immunity inducer can be used for improving the resistance of plants to biotic and abiotic stresses, effectively stop the infection and reduce the pathogenic levels of fungi, viruses and bacteria on plants, and at the same time, significantly improve the tolerance of plants to high temperature, low temperature, drought and salt stress. 2-amino-3-methylhexanoic acid has the features of being safe, environmentally friendly, and highly efficient.