Patents by Inventor Shiguo Chen

Shiguo Chen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11952436
    Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: April 9, 2024
    Assignee: ZHEJIANG UNIVERSITY
    Inventors: Shiguo Chen, Weiwei Hu, Xingqian Ye, Huan Cheng, Donghong Liu, Chantapakul Thunthacha
  • Publication number: 20240057598
    Abstract: Disclosed is use of 2-amino-3-methylhexanoic acid as a plant immunity inducer and in the preparation of a plant immunity inducer. The development of 2-amino-3-methylhexanoic acid as an active substance into a plant immunity inducer can be used for improving the resistance of plants to biotic and abiotic stresses, effectively stop the infection and reduce the pathogenic levels of fungi, viruses and bacteria on plants, and at the same time, significantly improve the tolerance of plants to high temperature, low temperature, drought and salt stress. 2-amino-3-methylhexanoic acid has the features of being safe, environmentally friendly, and highly efficient.
    Type: Application
    Filed: December 20, 2021
    Publication date: February 22, 2024
    Applicants: NANJING AGRICULTURAL UNIVERSITY, NANJING TIANNONG BIOTECHNOLOGY CO., LTD.
    Inventors: Shiguo CHEN, Qian YANG, Liru MI, He WANG, Jingjing LI, Wanping FANG, Aiping GUO
  • Publication number: 20230210934
    Abstract: Provided are a lycium barbarum leaf polysaccharide rich in galacturonic acid and a preparation method and use thereof. The method includes: mixing a lycium barbarum leaf and an acetone aqueous solution to obtain a mixture, and performing a fading treatment on the mixture to obtain a faded lycium barbarum leaf; extracting the faded lycium barbarum leaf with a chelating agent solution to obtain an extract solution; subjecting the extract solution to an alcohol precipitation with ethanol to obtain an alcohol precipitate; and subjecting the alcohol precipitate to an alcohol washing, a water redissolution, a dialysis and a drying in sequence to obtain the lycium barbarum leaf polysaccharide rich in galacturonic acid.
    Type: Application
    Filed: February 23, 2022
    Publication date: July 6, 2023
    Applicant: Zhejiang University
    Inventors: Shiguo Chen, Chengxiao Yu, Xinxin Hu, Jianle Chen, Haibo Pan, Xingqian Ye, Donghong Liu, Tian Ding
  • Publication number: 20230141130
    Abstract: The present disclosure belongs to the technical field of food processing and provides a phenolic stabilized fruit juice and a preparation method thereof. In the present disclosure, plant RG-I pectin polysaccharide is extracted through an ultra-high pressure technology, and then ultra-high pressure technology is used to promote the interaction of pectin polysaccharides and phenols in fruit juice, improve the stability of phenolic substances in fruit juice, and can improve the bioavailability of phenolic substances in fruit juice. The method of the present disclosure improves the added value of products and solves the problem of low bioavailability of phenolic substances in juice.
    Type: Application
    Filed: January 17, 2022
    Publication date: May 11, 2023
    Inventors: Zhiqiang Hou, Ziqi Chai, Xipan Shu, Luqin Luan, Shiguo Chen, Xingqian Ye
  • Publication number: 20230119640
    Abstract: The present disclosure provides an antibacterial hydrophilic compound. The antibacterial hydrophilic compound may react, induced by light through a hydrogen abstraction group in the structural formula thereof, with a C—H group and thus bind to a surface of a material having the C—H group (for example, chemical fibers such as polyester, chinlon, and the like; plastics, rubbers, and other similar materials), which can impart a durable antibacterial activity and hydrophilicity to the material. The antibacterial hydrophilic compound has a relatively strong binding force to the surface of the material without damaging the mechanical properties of the raw material. The present disclosure also provides a modified material that is modified by the antibacterial hydrophilic compound.
    Type: Application
    Filed: November 21, 2022
    Publication date: April 20, 2023
    Applicant: SHENZHEN UNIVERSITY
    Inventors: Shiguo CHEN, Shaobo ZHANG, Xiaohua CAI, Zaochuan GE
  • Patent number: 11503828
    Abstract: The present disclosure provides an antibacterial hydrophilic compound. The antibacterial hydrophilic compound may react, induced by light through a hydrogen abstraction group in the structural formula thereof, with a C—H group and thus bind to a surface of a material having the C—H group (for example, chemical fibers such as polyester, chinlon, and the like; plastics, rubbers, and other similar materials), which can impart a durable antibacterial activity and hydrophilicity to the material. The antibacterial hydrophilic compound has a relatively strong binding force to the surface of the material without damaging the mechanical properties of the raw material. The present disclosure also provides a modified material that is modified by the antibacterial hydrophilic compound.
    Type: Grant
    Filed: November 29, 2019
    Date of Patent: November 22, 2022
    Assignee: SHENZHEN UNIVERSITY
    Inventors: Shiguo Chen, Shaobo Zhang, Xiaohua Cai, Zaochuan Ge
  • Patent number: 11485799
    Abstract: The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: November 1, 2022
    Assignee: ZHEJIANG UNIVERSITY
    Inventors: Xingqian Ye, Weiwei Hu, Shiguo Chen, Huan Cheng, Donghong Liu
  • Publication number: 20220324827
    Abstract: An extraction method of proanthocyanidins from Myrica rubra Sieb. Et Zucc leaves is provided. In the method, proanthocyanidins from Myrica rubra Sieb. Et Zucc leaf are obtained by preparing an aqueous Myrica rubra Sieb. Et Zucc leaf suspension and centrifuging. In the method, only water is used as an extraction reagent without introduction of an organic extraction solvent, which reduces costs and is free from environmental pollution; moreover, the method does not cause organic reagent residue in a product, such that the biological activity of the proanthocyanidins is not affected and product safety is improved without affecting product quality; meanwhile, extraction steps are simplified, extraction time is reduced and extraction efficiency is improved. The method provides stability, good reproducibility, simple equipment and easy control of conditions.
    Type: Application
    Filed: August 25, 2020
    Publication date: October 13, 2022
    Inventors: Shiguo CHEN, Haibo PAN, Wenyang TAO, Xingqian YE
  • Publication number: 20220112313
    Abstract: The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.
    Type: Application
    Filed: December 3, 2019
    Publication date: April 14, 2022
    Inventors: XINGQIAN YE, WEIWEI HU, SHIGUO CHEN, HUAN CHENG, DONGHONG LIU
  • Publication number: 20220112314
    Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.
    Type: Application
    Filed: December 3, 2019
    Publication date: April 14, 2022
    Inventors: SHIGUO CHEN, WEIWEI HU, XINGQIAN YE, HUAN CHENG, DONGHONG LIU, CHANTAPAKUL THUNTHACHA
  • Patent number: 11292852
    Abstract: The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature.
    Type: Grant
    Filed: September 26, 2017
    Date of Patent: April 5, 2022
    Assignee: ZHEJIANG UNIVERSITY
    Inventors: Shiguo Chen, Hua Zhang, Xingqian Ye, Donghong Liu, Jiaqi Zheng
  • Publication number: 20220056159
    Abstract: Disclosed is a method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, including the following steps: adding fresh citrus fruit peels to alkali/acid processing water from citrus fruit canning for extracting; filtering the resulting mixture, adding to the resulting filtrate 95% ethanol 1 to 3 times the volume of the filtrate, and then adjusting the pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; filtering the product after standing, washing the resulting precipitate with 50% to 70% ethanol, and drying and crushing, thereby obtaining pectin. The method makes full use of waste resources from citrus fruit canning, solves the problem of pollution by processing discharge water, and has the advantages of saving the preparation cost of pectin from citrus peels, and improving the solubility of recovered pectin.
    Type: Application
    Filed: June 9, 2020
    Publication date: February 24, 2022
    Inventors: Jianle Chen, Xingqian Ye, Huan Cheng, Shiguo Chen, Donghong Liu
  • Patent number: 11252964
    Abstract: A textile having a surface that is coated with a compound of formula I or formula II.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: February 22, 2022
    Assignee: SHENZHEN UNIVERSITY
    Inventors: Shiguo Chen, Lingjun Yuan, Xingli Zhu, Shaojun Chen, Zaochuan Ge
  • Publication number: 20220030870
    Abstract: The present disclosure provides a compound and methods of preparing the compound. The compound has anti-microbial and anti-mite properties and can be bonded to materials such as fiber and synthetic polymer. The compound may also be used in a finishing agent for textiles, fibers, or yarns to protect these materials against microorganisms and mites. Additionally, when these materials are treated with a relatively low dosage of the compound, long-lasting and effective anti-mold and anti-mite effects may be achieved at a relatively low cost.
    Type: Application
    Filed: September 13, 2021
    Publication date: February 3, 2022
    Applicant: SHENZHEN UNIVERSITY
    Inventors: Shiguo CHEN, Jingwei GU
  • Patent number: 11202797
    Abstract: The present invention discloses a mixture of fucosylated chondroitin sulfate oligosaccharides having an average molecular weight between 2700 Da and 3600 Da, in which 90% of total components has a molecular weight between 1800 Da and 5400 Da. The mixture of fucosylated chondroitin sulfate oligosaccharide of the present invention has very low anti-FIIa factor activity, very low anti-FXa factor activity, very high anti-FXase factor activity, i.e., having a significant activity in selectively inhibiting intrinsic FXase, and a significant antithrombotic activity and no risk of bleeding and hypotension that are proved through animal experiments. The present invention also discloses a method for rapidly producing fucosylated chondroitin sulfate oligosaccharides by using sea cucumber as raw material, and the method includes: intercepting and concentrating crude polysaccharide with a membrane, deacetylation with hydrazine, pyrolysis with nitrous acid, reduction with sodium borohydride, membrane separation, etc.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: December 21, 2021
    Assignee: ZHEJIANG UNIVERSITY
    Inventors: Shiguo Chen, Lufeng Yan, Danli Wang, Junhui Li, Xingqian Ye, Donghong Liu
  • Patent number: 11110143
    Abstract: The present disclosure discloses a method for preparing a citrus-derived complex prebiotic agent. The method comprises the following steps: removing external citrus peels after blanching citrus fruits with hot water, soaking citrus segments after splitting with an acid solution and an alkali solution to remove membranes of the citrus segments, and adjusting the pH of the obtained alkali soaking water with acid soaking water or acid to be weakly acidic or neutral to obtain the complex prebiotic agent, which has the function of reversing intestinal flora disorders. Compared with the traditional prebiotic product preparing method, the product prepared by the method contains prebiotic functional components with different targets such as pectin, flavonoids, oligosaccharides, and other fibers at the same time, and has the efficacies of regulating intestinal flora and reversing intestinal flora disorders caused by antibiotics.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: September 7, 2021
    Assignee: ZHEJIANG UNIVERSITY
    Inventors: Shiguo Chen, Jiaqi Zheng, Xingqian Ye, Donghong Liu, Hua Zhang
  • Patent number: 11044908
    Abstract: A reactive antibacterial compound and a preparation method thereof are provided herein. The reactive antibacterial compound is represented by the general formula (I) or (II): wherein R1 represents OCN-L-NHCOOR?, OCN-L-NHCONHR?, OCN-L-NHCOSR?, OCN-L-COOR?, or OCN-L-COONHR?. G1 represents OCN-M-NHCOOG?, OCN-M-NHCONHG?, OCN-M-NHCOSG?, OCN-M-COOG?, or OCN-M-COONHG?. L, M, R? and G? independently for each occurrence represent divalent alkyl and cycloalkyl having from 1 to 18 carbon atoms, optionally substituted by up to 18 heteroatoms. R4 and G4 independently for each occurrence represent a divalent alkyl and cycloalkyl having from 1 to 18 carbon atoms, optionally substituted by at most 18 heteroatoms. G2 and G3 independently for each occurrence represent —H, —F, —Cl, —Br, —I, —OCH3, —OCH2CH3, —OPr, —CN, —SCN, —NO, —NO2, a monovalent unsubstituted or substituted alkyl, cycloalkyl, or aryl having from 1 to 7 carbon atoms. Z and X independently for each occurrence represent —COO, —SO3, or —OPO2OR5.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: June 29, 2021
    Assignee: SHENZHEN UNIVERSITY
    Inventors: Shiguo Chen, Lingjun Yuan
  • Publication number: 20210113645
    Abstract: The present disclosure discloses a method for preparing a citrus-derived complex prebiotic agent. The method comprises the following steps: removing external citrus peels after blanching citrus fruits with hot water, soaking citrus segments after splitting with an acid solution and an alkali solution to remove membranes of the citrus segments, and adjusting the pH of the obtained alkali soaking water with acid soaking water or acid to be weakly acidic or neutral to obtain the complex prebiotic agent, which has the function of reversing intestinal flora disorders. Compared with the traditional prebiotic product preparing method, the product prepared by the method contains prebiotic functional components with different targets such as pectin, flavonoids, oligosaccharides, and other fibers at the same time, and has the efficacies of regulating intestinal flora and reversing intestinal flora disorders caused by antibiotics.
    Type: Application
    Filed: September 27, 2018
    Publication date: April 22, 2021
    Inventors: Shiguo CHEN, Jiaqi ZHENG, Xingqian YE, Donghong LIU, Hua ZHANG
  • Publication number: 20210106030
    Abstract: A process for processing mandarin segments with both acidic and alkaline process water being directly utilized uses an organic acid or an inorganic polyacid instead of a monobasic inorganic acid. Mandarin segments, obtained by separating peeled mandarin into the segments, are treated by the organic acid or inorganic polyacid solution, and then subjected to removal of segment membranes in an alkali solution. The acidic and alkaline water after treatment is collected directly. The collected water can then be filtered and mixed for making an electrolyte beverage or a beverage base, containing multiple functional ingredients such as pectin, flavonoid, pigment, and limonin. Also, the collected water can be used to make dietary fiber having an activity of improving intestinal function.
    Type: Application
    Filed: January 13, 2020
    Publication date: April 15, 2021
    Applicant: ZHEJIANG UNIVERSITY
    Inventors: Xingqian Ye, Jianle Chen, Shiguo Chen
  • Publication number: 20200283547
    Abstract: The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature.
    Type: Application
    Filed: September 26, 2017
    Publication date: September 10, 2020
    Inventors: Shiguo CHEN, Hua ZHANG, Xingqian YE, Donghong LIU, Jiaqi ZHENG