Abstract: Disclosed herein are particles of an active ingredient, a composition comprising the same, and a method for producing the same. The method disclosed herein comprises the steps of, (a) forming a first solution by dissolving the active ingredient in a first solvent; (b) forming the particles of the active ingredient by mixing a second solvent with the first solution to produce an emulsified solution. Moreover, the second solvent is miscible with the first solvent, but is immiscible with the first solution.

Abstract: Disclosed herein is a method for producing microparticles of an active ingredient via an in-line recirculating mixing system, wherein the in-line recirculating mixing system comprises a mixer and a conduit couple to the mixer. The method disclosed herein comprises the steps of: (a) forming a continuous phase of a medium in the in-line recirculating mixing system; (b) allowing the continuous phase of the medium of the step (a) to come into contact with a first mixture of the active ingredient, a polymer and a solvent at a site in the conduit, thereby forming a second mixture, in the conduit; and (c) allowing the second mixture of the step (b) to enter the mixer and circulate in the in-line recirculating mixing system until the microparticles of the active ingredient are formed.

Abstract: Disclosed herein are expression constructs and methods for producing a protein of interest. According to some examples, an expression construct includes a nucleotide sequence encoding a fusion protein. To produce the protein of interest, the expression construct is transformed into a host cell, which is cultured under conditions that allow the expression of the fusion protein. The fusion protein has at least one expression unit; each expression unit has, sequentially, an affinity tag moiety, a spacer moiety, an enzymatic cleavage site, and a protein of interest moiety. The fusion protein is isolated from the host cell, solubilized, and refolded. The refolded fusion protein is cleaved to release the protein of interest.

Abstract: Impinging fluid jet streams are used in a continuous crystallization process to achieve high intensity micromixing of fluids so as to form a homogeneous composition prior to the start of nucleation. This process permits direct crystallization of high surface area particles of high purity and stability.