Abstract: An apparatus for monitoring experimental animals is provided in a rack including cages each for confining an experimental animal. The apparatus includes at least one rail unit including a rail group having a first rail disposed at one side of the cage; at least one drive unit electrically connected to the at least one rail unit and including a drive disposed at one side of the first rail and operatively connected thereto so that the drive is configured to activate the first rail; at least one control unit including a controller disposed at the other side of the first rail, a first mounting board disposed on a bottom of the controller, and a second mounting board secured to the first mounting board and disposed on the first rail; and at least one sensor unit including a sensor secured to the controller and electrically connected thereto.

Abstract: A method of monitoring experimental animals includes the steps of (1) obtaining image data from a self-moved device to form an image data set; (2) converting key frames of the image data of the image data set to still images; (3) performing a GUI program of a computer to label a coordinate of a target object of the still images as a label data and storing the label data in the computer, and labeling the label data as a target data set, (4) inputting data of the target data set into a machine learning platform and establishing an identification model; (5) placing the identification model in at least one control unit; and (6) forming a new target data set by subjecting a new image data to steps (2) and (3), and comparing the new target data set with the identification model to obtain an identification result.

Abstract: A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

Abstract: A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.