Abstract: The present invention provides a method of SNP detection by using DASH technique in bead-based microfluidics comprising following steps: (a) immobilizing a target single-strand DNA onto a microbead; (b) hybridizing the target single-strand DNA with an allele-specific probe; (c) intercalating a dye into a target-probe duplex region; (d) delivering the microbead into a microchannel; (e) heating the microbead to denature a hybridized DNA obtained from the step (c); (f) monitoring a fluorescence intensity of the hybridized DNA during the step (e) to obtain a melting curve; and (g) determining the SNP by a melting curve analysis method. Also, the present invention offers a rapid genotyping detection scheme with minimal amount of the reagents by confining the microbeads into designed fluidic traps and performing melting curve analysis controlled by a temperature control platform. The trapping mechanism was validated and optimized.