Abstract: A feeding analysis system of milk-production livestock includes a plurality of image analysis devices and a central server signally connected to each image analysis device. Each image analysis device is configured to capture a plurality of images of a feeding field, perform a clear processing on the images, identify a plurality of facial images of the plurality of milk-production livestock for feeding action from the cleared images and generate a plurality of capturing signals by capturing the plurality of facial images and a feeding duration corresponding to the facial images, and transmit the capturing signals to the central server. The central server is configured to receive the capturing signals, identify the milk-production livestock identities of the facial images of the corresponding capturing signal and generate a feeding record by integrating the identified milk-production livestock identity and the feeding duration of the capturing signal.

Abstract: A dairy cowshed monitoring system includes a cooling system having a plurality of operating modes, at least one image sensor, at least one temperature/humidity sensor, and a control circuit. The image sensor is for collecting image data in a dairy cowshed and obtaining a cow drinking water frequency in the dairy cowshed. The temperature/humidity sensor is for collecting a temperature and a humidity in the dairy cowshed and calculating a temperature/humidity index according to the temperature and the humidity. The control circuit is coupled to the cooling system, the image sensor and the temperature/humidity sensor and for receiving the image data, the cow drinking water frequency and the temperature/humidity index and determining whether to activate and control the cooling system in one of the operating modes to cool the dairy cowshed according to the cow drinking water frequency and the temperature/humidity index.

Abstract: The present invention provides a method of SNP detection by using gene detection in bead-based microfluidics comprising following steps: (a) preparing a microbead with a duplex DNA; (b) inserting a dye into the duplex DNA; (c) delivering the microbead into a microchannel with a temperature gradient region; (d) heating the temperature gradient region to denature the duplex DNA; (e) monitoring a fluorescence intensity of the duplex DNA during the step (d) to obtain a melting curve; and (f) determining the SNP by a melting curve analysis method; wherein the duplex DNA is synthesized by a target single-strand DNA and an allele-specific probe. Furthermore, the present invention offers a temperature gradient region to enhance the measurement accuracy. The continuous-flow mechanism in this region is validated and optimized.

Abstract: The present invention provides a method of SNP detection by using gene detection in bead-based microfluidics comprising following steps: (a) preparing a microbead with a duplex DNA; (b) inserting a dye into the duplex DNA; (c) delivering the microbead into a microchannel with a temperature gradient region; (d) heating the temperature gradient region to denature the duplex DNA; (e) monitoring a fluorescence intensity of the duplex DNA during the step (d) to obtain a melting curve; and (f) determining the SNP by a melting curve analysis method; wherein the duplex DNA is synthesized by a target single-strand DNA and an allele-specific probe. Furthermore, the present invention offers a temperature gradient region to enhance the measurement accuracy. The continuous-flow mechanism in this region is validated and optimized.