Abstract: The present disclosure relates to the technical field of composite coating preparation, in particular to a method for plasma-assisted and multi-step continuous preparation of a diffusion layer/amorphous carbon film composite coating and use thereof. In the present disclosure, a high-temperature plasma carburizing/nitriding technology and a low-temperature plasma carbon coating technology are combined by a plasma activation technology with argon ion under gradient cooling, and the surface of a material is activated by multiple bombardment on the surface of the material with high-energy argon ions. In this way, a cluster-like porous and loose structure on a surface of the diffusion layer is removed.

Abstract: Disclosed are a stamping method and a deep-drawing die for an aluminum alloy liner of a large-volume hydrogen storage cylinder, which solve that technical problem of low production efficiency of processing the aluminum alloy liner of the large-volume hydrogen storage cylinder by adopting strong spin and thinning of an aluminum tube in the prior art. The stamping method includes heating and stamping an aluminum ingot into a cup-shaped rough blank, then cold deep drawing the cup-shaped rough blank for thinning and lengthening into an aluminium alloy liner, and the deep-drawing die includes terrace die and thinning dies.

Abstract: Provided is a method for preparing L-BPA, which includes steps of: reacting N-protected (S)-4-halophenylalanine of Formula I, a boronating agent, Grignard reagent and bis(2-dimethylaminoethyl)ether to obtain a reaction mixture, wherein the reaction mixture comprises N-protected (S)-4-boronophenylalanine of Formula II and the R2 group represents a protecting group; isolating the N-protected (S)-4-boronophenylalanine from the reaction mixture; and deprotecting the R2 group of the N-protected (S)-4-boronophenylalanine to obtain L-BPA, wherein the L-BPA has a structure of Formula III.

Abstract: The present disclosure discloses a boron neutron capture therapy system comprising: a boron neutron capture therapy device and an ?-amino acid-like boron trifluoride compound having a structure shown as formula (I) below: Wherein: R is selected from hydrogen, methyl, isopropyl, 1-methylpropyl, 2-methylpropyl, hydroxymethyl, 1-hydroxyethyl, benzyl or hydroxybenzyl; M is H or metal atom. The energy generated from the action of the neutron beam generated by the boron neutron capture therapy device on the ?-amino acid-like boron trifluoride compound destroys tumor cell DNA. In another aspect, the present disclosure discloses a use of an ?-amino acid-like boron trifluoride compound in the preparation of a medicament for tumor therapy.

Abstract: Provided is a method for preparing L-BPA, which includes steps of: reacting N-protected (S)-4-halophenylalanine of Formula I, a boronating agent, Grignard reagent and bis(2-methylaminoethyl)ether to obtain a reaction mixture, wherein the reaction mixture comprises N-protected (S)-4-boronophenylalanine of Formula II and the R2 group represents a protecting group; isolating the N-protected (S)-4-boronophenylalanine from the reaction mixture; and deprotecting the R2 group of the N-protected (S)-4-boronophenylalanine to obtain L-BPA, wherein the L-BPA has a structure of Formula III.

Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.

Abstract: Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.

Abstract: Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.

Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.

Abstract: The invention relates to a process for preparing a porous ceramic body, which process is based on a negative replica method. The invention further relates to a ceramic body obtainable by said method and to its use as a scaffold for tissue engineering.

Abstract: The invention relates to a process for preparing a porous ceramic body, which process is based on a negative replica method. The invention further relates to a ceramic body obtainable by said method and to its use as a scaffold for tissue engineering.