Abstract: Provided herein is a pleuromutilin derivative containing a cycloalkyl group and preparation method and application thereof. Specifically, the pleuromutilin is reacted with p-toluenesulfonyl chloride to obtain an intermediate I, and the intermediate I is reacted with 1-amino-2-methylpropane-2-thiol hydrochloride to obtain an intermediate II, and then the intermediate II is reacted with cycloalkylcarbonyl chloride to obtain a target derivative. The synthesis process of these derivatives is simple, with a high yield and simple purification. These derivatives can effectively inhibit activities of Staphylococcus aureus and Streptococcus, and have superior activities to the marketed Tiamulin, and they also have good antibacterial activities against drug-resistant bacteria. In particular, the treatment effect of the derivative 2 of the target derivative is significantly superior to the clinical drugs Tiamulin and Valnemulin.

Abstract: Disclosed are a stamping method and a deep-drawing die for an aluminum alloy liner of a large-volume hydrogen storage cylinder, which solve that technical problem of low production efficiency of processing the aluminum alloy liner of the large-volume hydrogen storage cylinder by adopting strong spin and thinning of an aluminum tube in the prior art. The stamping method includes heating and stamping an aluminum ingot into a cup-shaped rough blank, then cold deep drawing the cup-shaped rough blank for thinning and lengthening into an aluminium alloy liner, and the deep-drawing die includes terrace die and thinning dies.

Abstract: Provided is a method for preparing L-BPA, which includes steps of: reacting N-protected (S)-4-halophenylalanine of Formula I, a boronating agent, Grignard reagent and bis(2-dimethylaminoethyl)ether to obtain a reaction mixture, wherein the reaction mixture comprises N-protected (S)-4-boronophenylalanine of Formula II and the R2 group represents a protecting group; isolating the N-protected (S)-4-boronophenylalanine from the reaction mixture; and deprotecting the R2 group of the N-protected (S)-4-boronophenylalanine to obtain L-BPA, wherein the L-BPA has a structure of Formula III.

Abstract: The present disclosure discloses a boron neutron capture therapy system comprising: a boron neutron capture therapy device and an ?-amino acid-like boron trifluoride compound having a structure shown as formula (I) below: Wherein: R is selected from hydrogen, methyl, isopropyl, 1-methylpropyl, 2-methylpropyl, hydroxymethyl, 1-hydroxyethyl, benzyl or hydroxybenzyl; M is H or metal atom. The energy generated from the action of the neutron beam generated by the boron neutron capture therapy device on the ?-amino acid-like boron trifluoride compound destroys tumor cell DNA. In another aspect, the present disclosure discloses a use of an ?-amino acid-like boron trifluoride compound in the preparation of a medicament for tumor therapy.

Abstract: Provided is a method for preparing L-BPA, which includes steps of: reacting N-protected (S)-4-halophenylalanine of Formula I, a boronating agent, Grignard reagent and bis(2-methylaminoethyl)ether to obtain a reaction mixture, wherein the reaction mixture comprises N-protected (S)-4-boronophenylalanine of Formula II and the R2 group represents a protecting group; isolating the N-protected (S)-4-boronophenylalanine from the reaction mixture; and deprotecting the R2 group of the N-protected (S)-4-boronophenylalanine to obtain L-BPA, wherein the L-BPA has a structure of Formula III.

Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.

Abstract: Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.

Abstract: Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.

Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.

Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.

Abstract: The invention relates to a process for preparing a porous ceramic body, which process is based on a negative replica method. The invention further relates to a ceramic body obtainable by said method and to its use as a scaffold for tissue engineering.

Abstract: The invention relates to a process for preparing a porous ceramic body, which process is based on a negative replica method. The invention further relates to a ceramic body obtainable by said method and to its use as a scaffold for tissue engineering.