Patents by Inventor Shihui Yang
Shihui Yang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240093247Abstract: A genetically engineered strain of Z. mobilis and uses thereof are provided. The genetically engineered strain named ZMNP is obtained by knockouts of four endogenous plasmids of pZM32, pZM32, pZM36, pZM33, and pZM39 from strain ZM4-Cas12a. ZMNP contains a reduced genome and has a series of excellent traits, such as high transformation efficiency, enhanced tolerance to inhibitors, improved capability to use secondary mother liquor and other excellent performance. ZMNP can be used as a chassis cell to construct diverse cell factories for biochemical production using different feedstocks especially the lignocellulosic biomass.Type: ApplicationFiled: September 18, 2023Publication date: March 21, 2024Applicant: Hubei UniversityInventors: Shihui YANG, Binan GENG, Qiaoning HE, Jun DU, Mian LI
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Patent number: 11879124Abstract: A construction method of a regulation system for gene expression in Zymomonas mobilis and applications are provided. The T7 expression system in Zymomonas mobilis has been constructed. The T7 expression system is used to construct a regulation circuit, help analyze the function of toxic genes and realize the regulation of metabolic pathways in Zymomonas mobilis. At the same time, shuttle plasmids that can be used in Zymomonas mobilis and Escherichia coli, also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in Zymomonas mobilis, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.Type: GrantFiled: May 10, 2023Date of Patent: January 23, 2024Assignee: Hubei UniversityInventors: Shihui Yang, Yi Wang, Yunhao Chen, Yueyue Chen, Qiaoning He
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Publication number: 20230407242Abstract: The disclosure relates to a genetically engineered Z. mobilis ZM4 for producing PHB and uses thereof. A variety of strains capable of producing both PHB and ethanol that are constructed by integrating three codon-optimized essential genes for exogenous PUB biosynthesis, phaA, phaB and phaC, together with a tetracycline-inducible promoter Ptet into the genome of ZM4.Type: ApplicationFiled: December 27, 2022Publication date: December 21, 2023Applicant: Hubei UniversityInventors: Shihui YANG, Qiaoning HE, Yang LI
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Publication number: 20230287398Abstract: A construction method of a tight regulation system for gene expression in Zywmmonas mobilis and applications are provided. The T7 expression system in Zymomonas mobilis has been constructed. The T7 expression system is used to construct a tight regulation circuit, help analyze the function of toxic genes and realize the tight regulation of metabolic pathways in Zymomonas mobilis. At the same time, a shuttle plasmids that can be used in Zymomonas mobilis and Escherichia coli., also be constructed to facilitate the construction of plasmid and improve the expression efficiency of foreign genes in Zymomonas mobilis, laying a foundation for subsequent protein expression and secretion and metabolic engineering pathway optimization regulation.Type: ApplicationFiled: May 10, 2023Publication date: September 14, 2023Applicant: Hubei UniversityInventors: Shihui YANG, Yi WANG, Yunhao CHEN, Yueyue CHEN, Qiaoning HE
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Publication number: 20220348939Abstract: The invention belongs to the technical field of genetic engineering, and particularly to a type I-F CRISPR-Cas system based on Zymomonas mobilis (Z. mobilis) including: four CRISPR structural sequences and one cas gene cluster, wherein the cas gene cluster comprises casi gene, cas3 gene, csyl gene, csy2 gene, csy3 gene and csy4 gene, wherein the cast-3 gene is a fusion form fused by a cast gene and a cas3 gene. The purpose of the present invention is to use Z. mobilis as a model bacterium, using a CRISPR-Cas system encoded by the genome of the Z. mobilis and exogenous CRISPR-Cas12a system to build a genome editing platform so as to provide a set of powerful tools for carrying out basic and applied research in this bacterium and similar cells, and promoting the development of metabolic engineering, systems biology and synthetic biology.Type: ApplicationFiled: October 22, 2019Publication date: November 3, 2022Inventors: Shihui Yang, Wenfang Peng, Wei Shen, Yanli Zheng, Li Yi, Lixin Ma
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Patent number: 11248258Abstract: A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of Z.Type: GrantFiled: May 9, 2019Date of Patent: February 15, 2022Assignee: Hubei UniversityInventors: Shihui Yang, Yongfu Yang, Wei Shen, Runxia Li, Ju Huang, Yu Wang, Li Yi, Lixin Ma
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Publication number: 20200385795Abstract: A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of Z.Type: ApplicationFiled: May 9, 2019Publication date: December 10, 2020Inventors: Shihui Yang, Yongfu Yang, Wei Shen, Runxia Li, Ju Huang, Yu Wang, Li Yi, Lixin Ma
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Patent number: 9206445Abstract: Disclosed herein are biocatalysts for the production of biofuels, including microorganisms that contain genetic modifications conferring tolerance to growth and fermentation inhibitors found in many cellulosic feedstocks. Methods of converting cellulose-containing materials to fuels and chemicals, as well as methods of fermenting sugars to fuels and chemicals, using these biocatalysts are also disclosed.Type: GrantFiled: April 29, 2014Date of Patent: December 8, 2015Assignee: Alliance for Sustainable Energy, LLCInventors: Shihui Yang, Jeffrey Linger, Mary Ann Franden, Philip T. Pienkos, Min Zhang
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Publication number: 20140342421Abstract: Disclosed herein are biocatalysts for the production of biofuels, including microorganisms that contain genetic modifications conferring tolerance to growth and fermentation inhibitors found in many cellulosic feedstocks. Methods of converting cellulose-containing materials to fuels and chemicals, as well as methods of fermenting sugars to fuels and chemicals, using these biocatalysts are also disclosed.Type: ApplicationFiled: April 29, 2014Publication date: November 20, 2014Applicant: Alliance for Sustainable Energy, LLCInventors: Shihui YANG, Jeffrey LINGER, Mary Ann FRANDEN, Philip T. PIENKOS, Min ZHANG
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Patent number: 8865440Abstract: The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.Type: GrantFiled: November 29, 2012Date of Patent: October 21, 2014Assignee: UT-Batelle, LLCInventors: Steven D. Brown, Shihui Yang
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Patent number: 8790902Abstract: The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.Type: GrantFiled: August 20, 2012Date of Patent: July 29, 2014Assignee: UT-Batelle, LLCInventors: Steven D. Brown, Shihui Yang
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Patent number: 8629255Abstract: The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.Type: GrantFiled: May 20, 2011Date of Patent: January 14, 2014Assignee: UT-Battelle, LLCInventors: Steven Brown, Adam Guss, Shihui Yang, Tatiana Karpinets, Lee Lynd, Xiongjun Shao
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Publication number: 20120329112Abstract: The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.Type: ApplicationFiled: August 20, 2012Publication date: December 27, 2012Applicants: OAK RIDGE ASSOCIATED UNIVERSITIES, UT-BATTELLE, LLCInventors: Steven D. Brown, Shihui Yang
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Publication number: 20110287499Abstract: The present invention provides isolated nucleic acid molecules which encode a mutant acetaldehyde-CoA/alcohol dehydrogenase or mutant alcohol dehydrogenase and confer enhanced tolerance to ethanol. The invention also provides related expression vectors, genetically engineered microorganisms having enhanced tolerance to ethanol, as well as methods of making and using such genetically modified microorganisms for production of biofuels based on fermentation of biomass materials.Type: ApplicationFiled: May 20, 2011Publication date: November 24, 2011Applicant: UT- BATTELLEInventors: Steven Brown, Adam Guss, Shihui Yang, Tatiana Karpinets, Lee Lynd, Xiongjun Shao
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Publication number: 20100311137Abstract: The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.Type: ApplicationFiled: June 8, 2010Publication date: December 9, 2010Applicant: UT-BATTELLE, LLCInventors: Steven D. Brown, Shihui Yang
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Publication number: 20100291647Abstract: The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.Type: ApplicationFiled: April 29, 2010Publication date: November 18, 2010Applicant: UT-BATTELLE, LLCInventors: Steven D. Brown, Shihui Yang
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Publication number: 20100249234Abstract: A method of reducing virulence in a bacterium comprising at least one of a GacS/GacA-type system, a HrpX/HrpY-type system, a T3SS-type system, and a Rsm-type system, the method comprising contacting the bacterium with an effective amount of a phenylpropanoid-type inhibitory compound.Type: ApplicationFiled: April 10, 2008Publication date: September 30, 2010Applicant: UWM RESEARCH FOUNDATION, INC.Inventors: Ching-Hong Yang, Shihui Yang