Abstract: A preparation method of an Al—Ti—Nb—B intermediate alloy includes following steps. KBF4 powder, Nb powder, and Ti powder are ball milled to obtain mixed powder, the mixed powder is pressed to obtain a ball-milled block, an Al block is melted to obtain an Al melt, the ball-milled block is added to the Al melt followed by smelting to obtain alloy liquid, and the alloy liquid is cast to obtain the Al—Ti—Nb—B intermediate alloy as a refining agent. A high-energy ball milling process is introduced into the preparation method of the Al—Ti—Nb—B intermediate alloy, improving reaction activity and lowering a reaction threshold. An ?-Al grain can be refined to be less than 90 ?m due to uniform dispersion of refining nucleation particles of the refining agent, the refining agent has a great refining effect. The preparation method is simple with easy steps and operations, the Al—Ti—Nb—B intermediate alloy has high purity.

Abstract: The invention discloses a cell culture and experiment device used in the field of biological and genetic engineering experiment apparatus, comprising a cent distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment. The central distribution compartment is equipped with a distribution chamber and a piston which can be moved forward and backward in the distribution chamber to alter the working volume of the distribution chamber. At the bottom of the distribution chamber, the central distribution compartment is equipped with a distribution valve controlling the connectivity between the distribution chamber and any of the channels.

Abstract: The present invention discloses a device for cell culturing and processing, applicable in the field of biological and genetic engineering experiments, which comprises at least one central distribution compartment, at least one incubation compartment; at least one processing compartment, a number of ducts to transfer fluids between said compartments, wherein a distribution cavity and a piston capable of moving back and forth in said distribution cavity for altering the operational volume of said distribution cavity are provided within said central distribution compartment, and a distribution valve controlling the connection between said distribution cavity and said ducts is provided at one end of said distribution cavity within the distribution compartment. During operation, cells grow and multiply in a cell suspension contained in the incubation compartment.

Abstract: The invention discloses a cell culture and experiment device used in the field of biological and genetic engineering experiment apparatus, comprising a central distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment. The central distribution compartment is equipped with a distribution chamber and a piston which can be moved forward and backward in the distribution chamber to alter the working volume of the distribution chamber. At the bottom of the distribution chamber, the central distribution compartment is equipped with a distribution valve controlling the connectivity between the distribution chamber and any of the channels.