Abstract: The disclosure provides a super-resolution fast-scanning coherent Raman scattering imaging method. The method: using pump light and Stokes light; combining the pump light and the Stokes light to obtain combined light; expanding/collimating the combined light; the combined light after the expanding/collimating entering a galvanometer, passing through a group of a scanning lens/a tube lens and being focused on a back focal plane of a microobjective and incidenting into a biological sample, such that the biological sample is excited to emit anti-Stokes light; collecting the excited anti-Stokes light by a detector. This method is characterized by deflecting, at different angles, a single light spot focused on the microobjective through a diffractive optics group including DOE and a dispersive element, into a plurality of 1×N light spots to incident into the biological sample, such that the anti-Stokes light excited from smaller molecules and being condensed and filtered, is collected by the detector.

Abstract: The disclosure provides a super-resolution fast-scanning coherent Raman scattering imaging method. The method: using pump light and Stokes light; combining the pump light and the Stokes light to obtain combined light; expanding/collimating the combined light; the combined light after the expanding/collimating entering a galvanometer, passing through a group of a scanning lens/a tube lens and being focused on a back focal plane of a microobjective and incidenting into a biological sample, such that the biological sample is excited to emit anti-Stokes light; collecting the excited anti-Stokes light by a detector. This method is characterized by deflecting, at different angles, a single light spot focused on the microobjective through a diffractive optics group including DOE and a dispersive element, into a plurality of 1xN light spots to incident into the biological sample, such that the anti-Stokes light excited from smaller molecules and being condensed and filtered, is collected by the detector.