Abstract: A multi-parameter automatic blood cell counting device includes a basic information generating part, an immune cell subgroup detection part, an immunodynamic change analyzing part, a storage part, and display part. The basic information generating part acquires examination information about a blood count of blood components and a white blood cell image and CD classification analysis information about monoclonal antibodies that bind to lymphocyte surface antigens. The immune cell subgroup detection part detects information about immune cell subgroups required for analyzing an immune status of a subject based on the examination information and the CD classification analysis information.

Abstract: A method of treating a disease in a subject including: obtaining a blood sample from the subject, identifying and counting white blood cells in the blood sample, binding antibodies to cluster of differentiation (CD) markers on the white blood cells, capturing images of the white blood cells, detecting binding of the antibodies to the white blood cell based on labeling of the white blood cells in the images, and classifying the white blood cells into CD subgroups based on the CD markers, calculating a total number of white blood cells serving as immune cells in a body of the subject and a number of white blood cells in each immune cell CD subgroup based on the number of white blood cells counted and the CD classification, and determining an immune status of the subject based on a ratio of the immune cell subgroups and a number of cells in each of the immune cell subgroups by comparing to a corresponding ratio and corresponding numbers of cells in a normal subject, wherein a change compared to the normal subjec

Abstract: A method is provided capable of testing an immune state in more detail and in a simple and inexpensive manner to identify diseases such as cancer and to identify side-effects of treatment with an anticancer agent and the like, effects of immunotherapy, a state of prognosis, and the like. The method includes: a step of calculating the total number of white blood cells serving as immune cells in a body of a subject and the number of each of immune cell subgroups based on test information on blood counts of blood components and white blood cell images of the subject and analysis information on the CD classification of monoclonal antibodies that bind to surface antigens of white blood cells; and a step of analyzing the immune state of the subject based on a proportion and a change in the numbers of the immune cell subgroups.