Patents by Inventor Shimpei GOTOH

Shimpei GOTOH has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD FOR INDUCING DIFFERENTIATION OF ALVEOLAR EPITHELIAL CELLS
    Publication number: 20220186189
    Abstract: This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3? inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF? inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3? inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3? inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.
    Type: Application
    Filed: March 2, 2022
    Publication date: June 16, 2022
    Applicant: KYOTO UNIVERSITY
    Inventors: Shimpei GOTOH, Yuki YAMAMOTO, Satoshi KONISHI, Michiaki MISHlMA
  • Method for inducing differentiation of alveolar epithelial cells
    Patent number: 11299712
    Abstract: This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3? inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF? inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3? inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3? inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: April 12, 2022
    Assignee: KYOTO UNIVERSITY
    Inventors: Shimpei Gotoh, Yuki Yamamoto, Satoshi Konishi, Michiaki Mishima
  • METHOD FOR INDUCING ALVEOLAR EPITHELIAL PROGENITOR CELLS
    Publication number: 20210284968
    Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3? inhibitor, (2) a medium containing a BMP inhibitor and a TGF? inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3? inhibitor.
    Type: Application
    Filed: March 8, 2021
    Publication date: September 16, 2021
    Applicant: KYOTO UNIVERSITY
    Inventors: Kenji OSAFUNE, Shimpei GOTOH, Isao ITO, Michiaki MISHIMA
  • Method for inducing alveolar epithelial progenitor cells
    Patent number: 10961508
    Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3? inhibitor, (2) a medium containing a BMP inhibitor and a TGF? inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3? inhibitor.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: March 30, 2021
    Assignee: KYOTO UNIVERSITY
    Inventors: Kenji Osafune, Shimpei Gotoh, Isao Ito, Michiaki Mishima
  • Method of producing airway epithelial cells
    Patent number: 10377991
    Abstract: This invention provides a method for stably producing airway epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing airway epithelial cells from pluripotent stem cells comprising steps: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3? inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF? inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3? inhibitor; (5) subjecting the cells obtained after Step (3) to three-dimensional culture in a medium containing a GSK3? inhibitor, FGF10, and a ROCK inhibitor; and (6) subjecting the proximal airway epithelial progenitor cells obtained in Step (5) to three-dimensional culture in a medium containing a ROCK inhibitor.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: August 13, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Shimpei Gotoh, Yuki Yamamoto, Satoshi Konishi, Michiaki Mishima
  • METHOD FOR INDUCING DIFFERENTIATION OF AIRWAY EPITHELIAL CELLS
    Publication number: 20180112186
    Abstract: This invention provides a method for stably producing airway epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing airway epithelial cells from pluripotent stem cells comprising steps: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3? inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF? inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3? inhibitor; (5) subjecting the cells obtained after Step (3) to three-dimensional culture in a medium containing a GSK3? inhibitor, FGF10, and a ROCK inhibitor; and (6) subjecting the proximal airway epithelial progenitor cells obtained in Step (5) to three-dimensional culture in a medium containing a ROCK inhibitor.
    Type: Application
    Filed: March 18, 2016
    Publication date: April 26, 2018
    Applicant: Kyoto University
    Inventors: Shimpei GOTOH, Yuki YAMAMOTO, Satoshi KONISHI, Michiaki MISHlMA
  • METHOD FOR INDUCING DIFFERENTIATION OF ALVEOLAR EPITHELIAL CELLS
    Publication number: 20180051256
    Abstract: This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3? inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGF? inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3? inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3? inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.
    Type: Application
    Filed: March 2, 2016
    Publication date: February 22, 2018
    Applicant: KYOTO UNIVERSITY
    Inventors: Shimpei GOTOH, Yuki YAMAMOTO, Satoshi KONISHI, Michiaki MISHlMA
  • METHOD FOR INDUCING ALVEOLAR EPITHELIAL PROGENITOR CELLS
    Publication number: 20160068816
    Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3? inhibitor, (2) a medium containing a BMP inhibitor and a TGF? inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3? inhibitor.
    Type: Application
    Filed: April 14, 2014
    Publication date: March 10, 2016
    Inventors: Kenji OSAFUNE, Shimpei GOTOH, Isao ITO, Michiaki MISHIMA