Patents by Inventor Shin'ichi Mochizuki
Shin'ichi Mochizuki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10195270Abstract: This peptide/?-1,3-glucan complex includes ?-1,3-glucan and a peptide/polynucleotide conjugate in which an antigenic peptide is bonded covalently to a polynucleotide or a derivative thereof. The polynucleotide or derivative thereof of the peptide/polynucleotide conjugate bonds via a hydrogen bond with ?-1,3-glucan, forming a complex having a triple helix structure including a single molecular chain of the polynucleotide or derivative thereof and two molecular chains of the ?-1,3-glucan. Alternatively, the side chain of the ?-1,3-glucan and the antigenic peptide are bonded covalently formed by either a cycloaddition reaction between an alkyne and an azide derivative, or a reaction between a maleimide group or a vinyl sulfone group and a thiol group.Type: GrantFiled: December 25, 2014Date of Patent: February 5, 2019Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kazuo Sakurai, Shin-ichi Mochizuki, Hiromi Morishita
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Publication number: 20170007695Abstract: This peptide/?-1,3-glucan complex includes ?-1,3-glucan and a peptide/polynucleotide conjugate in which an antigenic peptide is bonded covalently to a polynucleotide or a derivative thereof. The polynucleotide or derivative thereof of the peptide/polynucleotide conjugate bonds via a hydrogen bond with ?-1,3-glucan, forming a complex having a triple helix structure including a single molecular chain of the polynucleotide or derivative thereof and two molecular chains of the ?-1,3-glucan. Alternatively, the side chain of the ?-1,3-glucan and the antigenic peptide are bonded covalently formed by either a cycloaddition reaction between an alkyne and an azide derivative, or a reaction between a maleimide group or a vinyl sulfone group and a thiol group.Type: ApplicationFiled: December 25, 2014Publication date: January 12, 2017Applicant: Japan Science and Technology AgencyInventors: Kazuo SAKURAI, Shin-ichi MOCHIZUKI, Hiromi MORISHITA
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Publication number: 20150148529Abstract: As a means for solving the problem of providing a nucleic acid conjugate that does not undergo degradation at a DNA-RNA bonding site even in vivo, provided is a nucleic acid conjugate comprising a single-stranded DNA and a double-stranded RNA, wherein the 3? position of the 3?-terminal deoxyribonucleotide residue of the single-stranded DNA is bonded to the 5? position of the 5?-terminal ribonucleotide residue of one of the ribonucleotide strands of the double-stranded RNA, and the hydroxyl group at the 2? position of the 5?-terminal nucleotide of the ribonucleotide strand, which is bonded to the single-stranded DNA, is substituted with an alkoxy group or a halogen atom, and/or the phosphate diester group between the 3? position of the first ribonucleotide bonded to the single-stranded DNA and the 5? position of the adjacent ribonucleotide is substituted with any of phosphorothioate group, dithiophosphate diester group and trithiophosphate diester group.Type: ApplicationFiled: June 19, 2013Publication date: May 28, 2015Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kazuo Sakurai, Shin-ichi Mochizuki, Motoko Tanaka, Sadaharu Higuchi
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Patent number: 7468268Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: GrantFiled: August 31, 2004Date of Patent: December 23, 2008Assignee: Daiichi Sankyo Co., Ltd.Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Patent number: 7276344Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method of production of the protein. The protein is produced by human embryonic lung fibroblasts and has molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis, respectively. The protein can be isolated and purified from culture medium of the said fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity to the protein or a method for determination of the protein concentration using the antibodies.Type: GrantFiled: February 25, 2004Date of Patent: October 2, 2007Assignee: Sankyo Co., Ltd.Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Patent number: 7252973Abstract: A novel protein and a process of producing the protein is provided. The protein is a glycoprotein having activity of suppressing the differentiation and/or maturation of adipocyte, having a molecular weight of about 45 kD under non-reducing conditions and about 28 kD and/or 23 kD under reducing conditions, and exhibiting affinity to heparin. A process of producing the protein comprising culturing human fibroblasts and purifying the culture broth by chromatography using an ion exchange column, affinity column, and reverse phase column. A cDNA encoding the protein and a process of producing the protein using the cDNA are also provided. The protein of the present invention is useful as a pharmaceutical composition for preventing or treating obesity or as an antigen for establishing immunological diagnosis, etc.Type: GrantFiled: April 9, 1999Date of Patent: August 7, 2007Assignee: Daiichi Pharmaceutical Co., Ltd.Inventors: Masaaki Goto, Akihiro Tomoyasu, Kazuki Yano, Fumie Kobayashi, Nobuaki Nakagawa, Hisataka Yasuda, Kyoji Yamaguchi, Masahiko Kinosaki, Shin-ichi Mochizuki, Tadashi Nakakarumai, Tomonori Morinaga, Eisuke Tsuda, Kanji Higashio
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Patent number: 7205397Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: GrantFiled: April 17, 1998Date of Patent: April 17, 2007Assignee: Sankyo, Co., Ltd.Inventors: Masaaki Goto, Eisuke Tsuda, Shin′ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Patent number: 7125686Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: GrantFiled: August 20, 1997Date of Patent: October 24, 2006Assignee: Sankyo Co., Ltd.Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Patent number: 6919434Abstract: A protein which inhibits osteoclast diffraction and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions an SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: GrantFiled: June 23, 1999Date of Patent: July 19, 2005Assignee: Sankyo Co., Ltd.Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Publication number: 20050124054Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: ApplicationFiled: November 3, 2004Publication date: June 9, 2005Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Publication number: 20050118682Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: ApplicationFiled: November 3, 2004Publication date: June 2, 2005Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Patent number: 6855808Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis[[, respectively]]. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: GrantFiled: September 3, 2002Date of Patent: February 15, 2005Assignee: Sankyo Co., Ltd.Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio
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Publication number: 20050026837Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.Type: ApplicationFiled: August 31, 2004Publication date: February 3, 2005Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsuga Ueda, Kanji Higashio
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Publication number: 20050026253Abstract: A novel protein and a process of producing the protein is provided. The protein is a glycoprotein having activity of suppressing the differentiation and/or maturation of adipocyte, having a molecular weight of about 45 kD under non-reducing conditions and about 28 kD and/or 23 kD under reducing conditions, and exhibiting affinity to heparin. A process of producing the protein comprising culturing human fibroblasts and purifying the culture broth by chromatography using an ion exchange column, affinity column, and reverse phase column. A cDNA encoding the protein and a process of producing the protein using the cDNA are also provided. The protein of the present invention is useful as a pharmaceutical composition for preventing or treating obesity or as an antigen for establishing immunological diagnosis, etc.Type: ApplicationFiled: July 28, 2004Publication date: February 3, 2005Applicant: DAIICHI PHARMACEUTICAL CO., LTD.Inventors: Masaaki Goto, Akihiro Tomoyasu, Kazuki Yano, Fumie Kobayashi, Nobuaki Nakagawa, Hisataka Yasuda, Kyoji Yamaguchi, Masahiko Kinosaki, Shin-ichi Mochizuki, Tadashi Nakakarumai, Tomonori Morinaga, Eisuke Tsuda, Kanji Higashio
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Publication number: 20050014229Abstract: A protein which inhibits osteoclast differentiation and/or maturation and a method of production of the protein. The protein is produced by human embryonic lung fibroblasts and has molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis, respectively. The protein can be isolated-and purified from culture medium of the said fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity to the protein or a method for determination of the protein concentration using the antibodies.Type: ApplicationFiled: August 31, 2004Publication date: January 20, 2005Inventors: Masaaki Goto, Eisuke Tsuda, Shin'ichi Mochizuki, Kazuki Yano, Fumie Kobayashi, Nobuyuki Shima, Hisataka Yasuda, Nobuaki Nakagawa, Tomonori Morinaga, Masatsugu Ueda, Kanji Higashio