Abstract: An object of the present invention is to provide a method for selecting cells, an apparatus for selecting cells, and an apparatus system for selecting cells, which enables evaluation of a damage resistance of the cells in a suspension culture system. According to the present invention, provided is a method for selecting cells including introducing cells into a micro flow passage and allowing the cells to pass through the micro flow passage, evaluating a damage resistance of the cells to damage received by the cells due to passing through the micro flow passage, and selecting the cells based on the evaluation of the damage resistance.

Abstract: Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 µm or smaller, and 0 < B/A ? 0.

Abstract: Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 ?m or smaller, and 0<B/A?0.

Abstract: A method for producing a product related to the specified technology includes adjusting the concentration of cells in a culture vessel to a value of from 3×107 cells/ml to 3×108 cells/ml; in a case in which the average diameter of single cells in the culture vessel is designated as A, adjusting the number proportion of cells having a single cell diameter of 1.4×A or greater in the culture vessel to 5% or less, and adjusting the number proportion of cells having a single cell diameter in the range of A±A/7 to 50% or more.

Abstract: A cell culture device (1) includes: a culture vessel (10 ) that accommodates a cell suspension containing cells and a culture medium; and a stirring device (30) that is provided at a bottom part of the culture vessel (10) to stir the cell suspension accommodated in the culture vessel (10). The stirring device (30) has a shaft part (32), and a rotating part (33) rotatable with the shaft part (32) as a rotation axis. The rotating part (33) has a hole part (36) into which the shaft part (32) is inserted, and a gap (37) of 100 ?m or more is secured between an interior wall demarcating the hole part (36) and the shaft part (32). A closing part (38) that closes an end part of the gap (37) in an axial direction of the rotation axis is provided.

Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

Abstract: In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane at an outer edge of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line from the apex to the plane to each point in the diaphragm and by the perpendicular line is denoted by an angle A, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex is denoted by HT, the thickness H satisfies a relationship of 1?HT/H?1.75 at any point where the angle A is from 0° to 75°.

Abstract: Provide are a cell culture method which includes using a culture vessel which contains a cell suspension containing cells and discharging a gas including 30% by volume or more of oxygen into the cell suspension from a sparger disposed in the culture vessel to culture the cells, in which an average hole diameter in a gas discharge portion in the sparger is 1 ?m or more and 300 ?m or less, and a surface area A (m2) in the gas discharge portion in the sparger, a volume X (m3) of the cell suspension in the culture vessel, and a flow rate Q (m3/min) of the gas that is discharged from the sparger satisfy a specific relationship, a product producing method including the cell culture method, and a cell culture device that enables the cell culture method.

Abstract: Provided is a product production method which includes: a step of culturing a cell which produces a product and is contained in a cell suspension accommodated in a culture vessel; a separation treatment step of extracting the cell suspension from the culture vessel and separating the cell suspension by a tangential filtration method using a separation membrane; a step of returning a return liquid to the culture vessel; a step of supplying a fresh medium into the culture vessel; and a step of collecting the product, and in which with respect to a live cell concentration Nc, a pore diameter Dp of the separation membrane, a filtration area S of the separation membrane, and a volume Vf of a primary side flow path of the separation membrane, a number density Nd of fine particles having a particle size of 8 Dp to 30 Dp other than live cells in the cell suspension satisfies Nc?Nd?S/(32×?×Vf×Dp2).

Abstract: A method for producing a product related to the specified technology includes adjusting the concentration of cells in a culture vessel to a value of from 3×107 cells/ml to 3×108 cells/ml; in a case in which the average diameter of single cells in the culture vessel is designated as A, adjusting the number proportion of cells having a single cell diameter of 1.4×A or greater in the culture vessel to 5% or less, and adjusting the number proportion of cells having a single cell diameter in the range of A±A/7 to 50% or more.

Abstract: Provided is a cell culture apparatus including a culture vessel that stores a cell suspension containing cells; a first filter part that has a first filter membrane that performs membrane separation treatment on the cell suspension extracted from the culture vessel; a first circulation flow path that allows components blocked by the first filter membrane to return to the culture vessel; a second filter part that has a second filter membrane that performs membrane separation treatment on components of the cell suspension permeated through the first filter membrane; a second circulation flow path that allows components permeated through the second filter membrane to return to the culture vessel; and a recovery flow path that recovers components blocked by the second filter membrane. In the cell culture apparatus, an average hole diameter of the first filter membrane is 20 ?m or smaller, and 0<B/A?0.

Abstract: Provided are a transparent base material film laminate that is used by being arranged on the viewing side of a polarizing plate of an image display device having a backlight light source and the polarizing plate, the laminate having a first transparent base material film and a second transparent base material film, in which a Re of the first transparent base material film is 4,000 nm or more, the laminate is arranged for use such that an angle formed between a slow axis of the first transparent base material film and an absorption axis of the polarizing plate is 45°±20° and such that an angle formed between a slow axis of the second transparent base material film and the absorption axis of the polarizing plate is 90°±30° or 0°±30°, an angle formed between the slow axes of the first transparent base material film and the second transparent base material film is neither 0° nor 90°, and the second transparent base material film is used by being arranged on the viewing side with respect to the first transparent b

Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

Abstract: A liquid crystal display device has a light source unit and a liquid crystal cell sandwiched by two polarizing plates. The light emitted by the light source unit has separated peaks in each of blue, green and red wavelength ranges. The green and red peaks have a full width at half maximum intensity of 20 nm or more. At least one of the polarizing plates has a polarizer and a protective film with a Re of 5,000 nm or more and low moisture permeability. The device exhibits excellent color reproducibility and prevents display nonuniformity under a high temperature and high humidity environment.

Abstract: Provided are a transparent base material film laminate that is used by being arranged on the viewing side of a polarizing plate of an image display device having a backlight light source and the polarizing plate, the laminate having a first transparent base material film and a second transparent base material film, in which a Re of the first transparent base material film is 4,000 nm or more, the laminate is arranged for use such that an angle formed between a slow axis of the first transparent base material film and an absorption axis of the polarizing plate is 45°±20° and such that an angle formed between a slow axis of the second transparent base material film and the absorption axis of the polarizing plate is 90°±30° or 0°±30°, an angle formed between the slow axes of the first transparent base material film and the second transparent base material film is neither 0° nor 90°, and the second transparent base material film is used by being arranged on the viewing side with respect to the first transparent b

Abstract: Provided are a white polyester film including a polyester and white particles, in which, at an equivalent of a thickness of 250 ?m, a machine stretching direction tear strength FMD is 2.5 to 6.0 N, a transverse stretching direction tear strength FTD is 2.0 to 5.0 N, a ratio of the machine stretching direction tear strength FMD to the transverse stretching direction tear strength FTD is 1.05 to 4.00, and a concentration of terminal carboxyl groups is 5 to 25 equivalents/ton, a method for manufacturing the same, a solar cell back sheet and a solar cell module in which the same white polyester film is used.

Abstract: The invention provides a polyester film including: a polyester substrate; and a layered portion that is disposed on at least one surface of the polyester substrate and has an elastic modulus of from 5 MPa to 800 MPa, a ratio of a peak intensity at 988 cm?1 that is observed by subjecting a surface of the polyester substrate to infrared spectroscopy to a peak intensity at 1410 cm?1 that is observed by subjecting a surface of the polyester substrate to infrared spectroscopy satisfying the following Inequality (I): 0.010?(peak intensity at 988 cm?1)/(peak intensity at 1410 cm?1)?0.040??(I).

Abstract: A liquid crystal display device has a light source unit and a liquid crystal cell sandwiched by two polarizing plates. The light emitted by the light source unit has separated peaks in each of blue, green and red wavelength ranges. The green and red peaks have a full width at half maximum intensity of 20 nm or more. At least one of the polarizing plates has a polarizer and a protective film with a Re of 5,000 nm or more and low moisture permeability. The device exhibits excellent color reproducibility and prevents display nonuniformity under a high temperature and high humidity environment.

Abstract: The invention provides a polyester film including: a polyester substrate; and a layered portion that is disposed on at least one surface of the polyester substrate and has an elastic modulus of from 5 MPa to 800 MPa, a ratio of a peak intensity at 988 cm?1 that is observed by subjecting a surface of the polyester substrate to infrared spectroscopy to a peak intensity at 1410 cm?1 that is observed by subjecting a surface of the polyester substrate to infrared spectroscopy satisfying the following Inequality (I): 0.010?(peak intensity at 988 cm?1)/(peak intensity at 1410 cm?1)?0.040??(I).

Abstract: A biaxially stretched polyester film having reduced occurrences of scratches and having superior hydrolysis resistance as compared with conventional polyester films is provided. The polyester film has: a film width of 1 m or more; an intrinsic viscosity (IV) of 0.70 dL/g or greater; a pre-peak temperature measured by differential scanning calorimetry (DSC) of from 160° C. to 210° C.; and a variation in a degree of crystallinity in a film width direction of from 0.3% to 5.0%.