Patents by Inventor Shinichi Ohnuma

Shinichi Ohnuma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mutated prenyl diphosphate synthases
    Patent number: 6316216
    Abstract: A mutant prenyl diphosphate synthase having a modified amino acid sequence of prenyl diphosphate synthase, wherein the amino acid residue located at the eighth position in the N-terminal direction from D of the N-terminal of the Asp-rich domain DDXX(XX)D (in the sequence X denotes any amino acid and the two X's in the parentheses may not be present) present in the region II has been substituted by another amino acid, or both of the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal and the amino acid residue located at the eighth position in the N-terminal direction from D of the N-terminal have been substituted by other amino acids.
    Type: Grant
    Filed: April 27, 1999
    Date of Patent: November 13, 2001
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Hiroyuki Nakane, Tokuzo Nishino, Shinichi Ohnuma, Kazutake Hirooka
  • Mutant prenyl diphosphate synthase, DNA encoding mutant prenyl diphosphate synthase and process for producing mutant prenyl phosphate synthase
    Patent number: 6225096
    Abstract: Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.
    Type: Grant
    Filed: December 30, 1999
    Date of Patent: May 1, 2001
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Keishi Narita, Chika Ishida, Yoshie Takeuchi, Chikara Ohto, Shinichi Ohnuma, Tokuzo Nishino
  • Mutant prenyl diphosphate synthase
    Patent number: 6040165
    Abstract: Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.
    Type: Grant
    Filed: July 1, 1997
    Date of Patent: March 21, 2000
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Keishi Narita, Chika Ishida, Yoshie Takeuchi, Chikara Ohto, Shinichi Ohnuma, Tokuzo Nishino
  • Farnesyl diphosphate synthase
    Patent number: 5935832
    Abstract: A mutant prenyl diphosphate synthase capable of synthesizing prenyl diphosphates, shorter than those synthesized by the original enzyme, by modifying the amino acid sequence in and upstream of the aspartic acid-rich domain DDXX(XX)D (X denotes any amino acid, and XX in the parentheses may not be present) present in region II of the prenyl diphosphate synthase.
    Type: Grant
    Filed: July 22, 1997
    Date of Patent: August 10, 1999
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Hiroyuki Nakane, Chikara Ohto, Shinichi Ohnuma, Kazutake Hirooka, Tokuzo Nishino
  • Process for the production of prenyl diphosphate using mutants of geranylgeranyl diphosphate synthase
    Patent number: 5885810
    Abstract: The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.
    Type: Grant
    Filed: April 1, 1998
    Date of Patent: March 23, 1999
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Chika Asada, Shinichi Ohnuma, Tokuzo Nishino, Kazutake Hirooka, Hisashi Hemmi
  • Nucleic acid encoding mutant geranylgeranyl diphosphate synthase
    Patent number: 5882909
    Abstract: The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein, at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.
    Type: Grant
    Filed: April 1, 1998
    Date of Patent: March 16, 1999
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Chika Asada, Shinichi Ohnuma, Tokuzo Nishino, Kazutake Hirooka, Hisashi Hemmi
  • Long-chain prenyl diphosphate synthase
    Patent number: 5807725
    Abstract: The present invention discloses a mutated enzyme comprising a geranylgeranil diphosphate synthase having its origin in wild type Sulfolobus acidocaldarius wherein, at least one of phenylalanine at position 77, methionine at position 85, valine at position 99, tyrosine at position 101, phenylalanine at position 118, arginine at position 199 and aspartic acid at position 312 is substituted with another amino acid.
    Type: Grant
    Filed: August 29, 1996
    Date of Patent: September 15, 1998
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Chika Asada, Shinichi Ohnuma, Tokuzo Nishino, Kazutake Hirooka, Hisashi Hemmi
  • Geranylgeranyl diphosphate synthase and DNA coding therefor
    Patent number: 5773273
    Abstract: DNA coding for thermostable geranylgeranyl diphosphate (GGDP) synthase derived from Sulfolobus acidocaldarius is provided. The DNA is useful for production of GGDP synthase, which is, in turn, useful for production of GGDP.
    Type: Grant
    Filed: March 24, 1995
    Date of Patent: June 30, 1998
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Tokuzo Nishino, Shinichi Ohnuma, Manabu Suzuki, Chikara Ohto, Chika Asada, Yuka Higuchi, Yoshie Takeuchi
  • Mutated farnesyldiphoshate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding therefor
    Patent number: 5766911
    Abstract: A mutated farnesyldiphosphate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding for said mutated enzyme, wherein the mutated enzyme is modified from a native farnesyldiphosphate synthase by mutation of a gene coding for a native farnesyldiphosphate synthase.
    Type: Grant
    Filed: September 28, 1995
    Date of Patent: June 16, 1998
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Ayumi Koike, Shusei Obata, Tokuzo Nishino, Shinichi Ohnuma, Takeshi Nakazawa, Kyozo Ogura, Tanetoshi Koyama
  • Mutant prenyl diphosphate synthase
    Patent number: RE43425
    Abstract: A mutant prenyl diphosphate synthase capable of synthesizing prenyl diphosphates, shorter than those synthesized by the original enzyme, by modifying the amino acid sequence in and upstream of the aspartic acid-rich domain DDXX (XX)D (X denotes any amino acid, and XX in the parentheses may not be present) present in region II of the prenyl diphosphate synthase.
    Type: Grant
    Filed: July 12, 2001
    Date of Patent: May 29, 2012
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Hiroyuki Nakane, Chikara Ohto, Shinichi Ohnuma, Kazutake Hirooka, Tokuzo Nishino