Abstract: A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nucleic acid binding protein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biological sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.

Abstract: A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): 1

Abstract: A method for analyzing an objective substance, comprsing reacting a labeled probe with an objective substance on a biological sample, said probe comprsing a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nudeic acid binding potein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biolgical sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.

Abstract: The present invention relates to a multicore plastic optical fiber for optical signal transmission, which comprises a plurality of cores made of a transparent resin; first cladding layers made of a transparent first cladding resin having a lower refractive index than said core resin, each of said first cladding layers coating said each core; and a second cladding resin surrounding said cores with said first cladding layers, and having a lower refractive index than said first cladding resin. The multicore plastic optical fiber of the present invention is suitably applicable to optical signal transmission since it receives a large quantity of light and exhibits a small light loss upon bending.

Abstract: A sugar ester derivative of the general formula (I) ##STR1## wherein G stands for a group of the formula (A) ##STR2## or a group of the formula (B) ##STR3## wherein .lambda. means 0 or 1; at least one of R.sub.1 and R.sub.2 means an ester residue of a saturated or unsaturated fatty acid having 1-30 carbon atoms and the other group means hydrogen atom or acetyl group, and R.sub.3 means a group of the formula (C) ##STR4## wherein X means a halogen atom, m means an integer of 0 to 4, Y means hydroxy group, an alkoxy group, a carboxyl group or sulfonic acid group, n means 0 or 1 and Z means nitro group or nitrovinyl group and its use.

Abstract: A novel glutamate dehydrogenase derived from the genus Pseudomonas which is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, is with an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP. A reagent composition for measuring an amount of ammonia characterized in containing a glutamate dehydrogenase which is not activated by ADP and is NADH-dependent, .alpha.-ketoglutaric acid or a salt thereof and NADH; and a reagent composition wherein urease or creatinine deiminase is further added to the above-mentioned reagent composition.

Abstract: A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.

Abstract: A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.

Abstract: A novel glutamate dehydrogenase is derived from the genus Pseudomonas and is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, has an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP.

Abstract: A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.

Abstract: The present invention relates to a multicore plastic optical fiber for light signal transmission comprising 7 or more cores having a diameter of 50 to 200 .mu.m which are covered with a cladding resin having a refractive index lower than that of the core resin by 0.005 to 0.04. The multicore plastic optical fiber of the present invention has low transmission loss in a broad transmission bandwidth and exhibits excellent bending characteristics. Therefore, it is suitable for transmission at a high speed in short and medium distances.

Abstract: A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.

Abstract: The present invention relates to a multicore hollow optical fiber comprising a hollow central part and a plastic peripheral part, more particularly, a multicore hollow optical fiber having a cross-section of the peripheral part wherein (a) an islands-in-sea structure is formed; (b) the islands comprise a core resin having at least higher refractive index than a cladding resin; (c) the sea comprises a cladding resin or the third resin; (d) the core resin is surrounded by the cladding resin; and (e) voids do not substantially exist; and also having, in the direction of the axis, the above cross-section continuously from one end to the other.

Abstract: Maltooligosaccharide derivatives represented by the general formula: ##STR1## wherein at least one of R.sup.1 and R.sup.2 denotes a radical represented by: R.sup.3 --S(O).sub.m --R.sup.4 or R.sup.3 --CO--R.sup.4, and the rest of them denotes hydrogen atom, an alkyl radical containing 1 to 6 carbon atoms or a phenyl radical, in which R.sup.3 is an alkyl radical containing 1 to 5 carbon atoms, R.sup.4 is an alkylene radical containing 1 to 5 carbon atoms, and m is 1 or 2, R.sup.5 denotes a substituted or non-substituted phenyl radical, and n denotes an integer of 1 to 8; and reagents comprising the maltooligosaccharide derivatives.

Abstract: A reagent for the determination of acid phosphatase in body fluids which contains as a substrate a compound of the formula: ##STR1## wherein X is a halogen atom, and n and m are each an integer of 1 to 4, and a method for the determination of acid phosphatase in body fluids using the reagent. The reagent is clinically useful for diagnosis of prostate diseases such as prostate carcinoma and prostatomegaly and observation of progress thereof.

Abstract: A plastic optical fiber comprising a polymer core constituted mainly of methyl methacrylate and a polymer cladding having a lower refractive index than that of the core, characterized in that the core consists of a methyl methacrylate homopolymer or a copolymer constituted of at least 95% by weight of methyl methacrylate and less than 5% by weight of (i) methyl acrylate, (ii) ethyl acrylate, or (iii) a mixture of both acrylates, that the weight-average molecular weight of the core polymer is from 80,000 to 200,000, and that light attenuations through the fiber are up to 250, 130, 80, and 130 dB/Km at wavelengths of 400, 450, 570, and 650 nm, respectively, and a process for producing the same.

Abstract: A cladding material for plastic optical fiber comprising a vinylidene fluoride-trifluoroethylene-hexafluoroacetone copolymer which comprises 3.5 to 6.5% by mole of hexafluoroacetone units, a molar ratio of vinylidene fluoride unit to trifluoroethylene unit of 3 to 6:1, and a melt index of 10 to 60 g/10 min, and a plastic optical fiber comprising said cladding material and a methyl methacrylate homopolymer or copolymer which comprises over 50% by weight of methyl methacrylate units as the core material.

Abstract: There is provided an improved quantitative analysis for the determination of a substrate or enzyme. In the analysis for the enzyme, it is reacted with a known substrate and in the analysis for the substrate, it is reacted with a known enzyme and then the resultant substance is reacted with an oxidase to produce hydrogen peroxide which is then reacted with a peroxidase, phenol derivatie and coupler to form a colored material which is then subjected to colorimetic analysis. The improvement resides in conducting the foregoing process by adding to the test sample a first reagent containing the oxidase, peroxidase and phenol derivative represented by the formula: ##STR1## wherein R.sub.1 is .circle.a a lower alkyl group having one to five carbon atoms or .circle.b the group defined in .circle.a above having a hydroxyl or sulfonic acid group, R.sub.