Abstract: A cell culture method includes performing adherent culture of cells on a coat layer layered on a surface of a cell culture container by placing a culture medium and the cells in the cell culture container. The coat layer includes a layer (i) that includes a layer (i-1) including gelatin or casein and a layer (i-2) that includes a polycationic material and a layer (ii) that is layered on the layer (i) and includes a cell adhesion factor. The layer (ii) is disposed at a position at which the layer (ii) comes into contact with the cells, or the coat layer includes the layer (i) and the layer (i) is disposed at a position at which the layer (i) comes into contact with the cells, and the culture medium includes the cell adhesion factor.

Abstract: A method of manufacturing a copolymer resin of dicyclopentadiene and a vinyl aromatic compound, by which the resin can be continuously manufactured with reduced generation of high molecular weight products of the vinyl aromatic compound and insoluble substances from dicyclopentadiene, and at high yield. The manufacturing method includes continuously injecting polymerization raw materials into a preheated solvent in the reaction system, and concurrently ejecting a part of the reaction solution containing the solvent and the polymerization raw materials outside the reaction system, while raising the temperature of the polymerization raw materials. This enables rapid rise of the temperature of the polymerization raw materials by utilizing sensible heat, resulting in reduced generation of insoluble substances. In addition, providing a reaction step following the raw material injection step allows the polymerization reaction to proceed until a desired molecular weight is achieved, resulting in improved yield.