Abstract: (Object) To improve the accuracy of the placement of liquid droplets. (Means of Achieving the object) A liquid droplet discharging method is performed by a liquid droplet discharging apparatus configured to discharge a liquid droplet from a nozzle hole formed in a film-like member, the liquid droplet discharging method including positioning the nozzle hole inside a recessed portion provided in a container; and discharging the liquid droplet from the nozzle hole positioned inside the recessed portion.

Abstract: A cell-containing structure is provided that allows ready-to-use nerve drug response evaluation with high reproducibility to be easily performed. The cell-containing structure for evaluating an electrical property of neurons includes: (a) a culture surface to which the neurons are able to be adhered; (b) a cell mass that is adhered to the culture surface and contains at least one of the neurons; and (c) a plurality of electrodes for measuring the electrical property of the cell mass, wherein a spontaneous firing frequency of cells contained in the cell mass is 0.25 Hz or more per electrode.

Abstract: A powder container includes a powder chamber for containing powder for forming images, a powder outlet formed in a face of the powder container, and a shutter assembly to open and close the powder outlet and including first and second shutters. The first shutter is movable between a sealing position to close the powder outlet and an open position to open the powder outlet and includes a pressed member to cancel retention of the first shutter at the sealing position. The second shutter includes a pressing projection that interferes with the pressed member of the first shutter and is movable between a shielding position to cover the pressed member without interference between the pressing projection and the pressed member and a releasing position to press the pressed member with the pressing projection.

Abstract: A powder container includes a powder chamber for containing powder for forming images, a powder outlet formed in a face of the powder container, and a shutter assembly to open and close the powder outlet and including first and second shutters. The first shutter is movable between a sealing position to close the powder outlet and an open position to open the powder outlet and includes a pressed member to cancel retention of the first shutter at the sealing position. The second shutter includes a pressing projection that interferes with the pressed member of the first shutter and is movable between a shielding position to cover the pressed member without interference between the pressing projection and the pressed member and a releasing position to press the pressed member with the pressing projection.

Abstract: The invention provides a cell-containing vessel comprising a plurality of neural cell-containing spheroids for which the variation calculated using the calculation method described below is less than 20%, wherein the neural cell-containing spheroids contain a plurality of types of cells including neural cells, (Calculation Method) Calcium transient assays are conducted for each of a plurality of neural cell-containing spheroids, a number of spontaneous oscillations in a 10-minute period is measured, an average and standard deviation for the number of spontaneous oscillations are calculated, and a variation is calculated using formula (1) below: Variation (%)=standard deviation for number of spontaneous oscillations/average number of spontaneous oscillations×100??(1).

Abstract: A method of producing a cell-containing container is provided, including a process in which pluripotent stem cells that have been induced to differentiate into neurons, and astrocytes are mixed and seeded in a cell culture container in which an electrode array is arranged on a culture surface, and a process in which the cell culture container is incubated, and as a result, the pluripotent stem cells and the astrocytes adhere to the culture surface, and the pluripotent stem cells differentiate into neurons.

Abstract: Provided is a cell-containing container including nerve cells and a medium, in which the nerve cells adhere to a culture surface of the cell-containing container, an adhesion area between the nerve cells and the culture surface is 0.5 mm2 or more per 80,000 nerve cells, and a concentration of glucose in the medium is 1 g/L or higher.

Abstract: A drying suppression device including: a main body housing a well plate; and an openable/closable lid provided on the main body, the main body including: a holder section that holds the well plate; a liquid storage tank that stores liquid; and a support section that supports the holder while keeping the holder section away from an inner bottom surface of the liquid storage tank, wherein, with the lid being closed, an inner space surrounded by the liquid storage tank and the lid communicate with an outside space.

Abstract: A cell-containing container includes at least one recessed part accommodating cells, in which the cells adhere to a bottom surface of the recessed part, and a cell density in a first region of the bottom surface is 250 to 20,000 cells/mm2, and a cell density in a second region surrounding a periphery of the first region is less than 250 cells/mm2.

Abstract: A cell culture vessel includes: a base member having a plurality of recesses; and a frame member having through-holes and configured to be detachable from and attachable to the base member, wherein when the frame member is stacked on the base member, the plurality of recesses and the through-holes communicate with each other, the frame member includes a plurality of kinds of frame members, and the plurality of kinds of frame members have mutually different combinations of the through-holes communicating with the plurality of recesses.

Abstract: Provided is a liquid discharging head including: a membranous member including a discharging port through which a liquid is discharged; and a displacement member disposed at membranous member's one side at which the liquid to be discharged through the discharging port is provided and configured to displace a position of the membranous member to cause the liquid to be discharged through the discharging port.

Abstract: Liquid discharge head comprising: liquid discharge unit, which includes discharge port, liquid retaining section configured to retain liquid to be discharged from the discharge port, and displacement section configured to discharge the liquid retained within the liquid retaining section from the discharge port; a pair of liquid storage sections, which are configured to store the liquid and are each connected to the liquid retaining section in the liquid discharge unit so that the liquid can flow; a pair of liquid feeding sections that are connected to the pair of liquid storage sections and are configured to feed the liquid between the liquid storage section and the liquid retaining section; and a pair of open and close sections that are each disposed at flow path between the liquid feeding section and the liquid storage section and are configured to open and close the flow path.

Abstract: A powder container includes a powder chamber for containing powder for forming images, a powder outlet formed in a face of the powder container, and a shutter assembly to open and close the powder outlet and including first and second shutters. The first shutter is movable between a sealing position to close the powder outlet and an open position to open the powder outlet and includes a pressed member to cancel retention of the first shutter at the sealing position. The second shutter includes a pressing projection that interferes with the pressed member of the first shutter and is movable between a shielding position to cover the pressed member without interference between the pressing projection and the pressed member and a releasing position to press the pressed member with the pressing projection.

Abstract: A powder container includes a powder chamber for containing powder for forming images, a powder outlet formed in a face of the powder container, and a shutter assembly to open and close the powder outlet and including first and second shutters. The first shutter is movable between a sealing position to close the powder outlet and an open position to open the powder outlet and includes a pressed member to cancel retention of the first shutter at the sealing position. The second shutter includes a pressing projection that interferes with the pressed member of the first shutter and is movable between a shielding position to cover the pressed member without interference between the pressing projection and the pressed member and a releasing position to press the pressed member with the pressing projection.

Abstract: Liquid discharge head comprising: liquid discharge unit, which includes discharge port, liquid retaining section configured to retain liquid to be discharged from the discharge port, and displacement section configured to discharge the liquid retained within the liquid retaining section from the discharge port; a pair of liquid storage sections, which are configured to store the liquid and are each connected to the liquid retaining section in the liquid discharge unit so that the liquid can flow; a pair of liquid feeding sections that are connected to the pair of liquid storage sections and are configured to feed the liquid between the liquid storage section and the liquid retaining section; and a pair of open and close sections that are each disposed at flow path between the liquid feeding section and the liquid storage section and are configured to open and close the flow path.

Abstract: A fabrication method includes laminating a fabrication material to form an object, and applying a release material on a surface of the object to form a release layer on the surface of the object. A surface free energy of the release material is equal to or smaller than 25 mN/m.

Abstract: A powder container includes a powder chamber for containing powder for forming images, a powder outlet formed in a face of the powder container, and a shutter assembly to open and close the powder outlet and including first and second shutters. The first shutter is movable between a sealing position to close the powder outlet and an open position to open the powder outlet and includes a pressed member to cancel retention of the first shutter at the sealing position. The second shutter includes a pressing projection that interferes with the pressed member of the first shutter and is movable between a shielding position to cover the pressed member without interference between the pressing projection and the pressed member and a releasing position to press the pressed member with the pressing projection.

Abstract: Provided is a liquid discharging head including: a membranous member including a discharging port through which a liquid is discharged; and a displacement member disposed at membranous member's one side at which the liquid to be discharged through the discharging port is provided and configured to displace a position of the membranous member to cause the liquid to be discharged through the discharging port.

Abstract: A powder container includes a powder chamber for containing powder for forming images, a powder outlet formed in a face of the powder container, and a shutter assembly to open and close the powder outlet and including first and second shutters. The first shutter is movable between a sealing position to close the powder outlet and an open position to open the powder outlet and includes a pressed member to cancel retention of the first shutter at the sealing position. The second shutter includes a pressing projection that interferes with the pressed member of the first shutter and is movable between a shielding position to cover the pressed member without interference between the pressing projection and the pressed member and a releasing position to press the pressed member with the pressing projection.

Abstract: Provided is a cell contained container including at least two concaves, wherein the concaves contain cells, wherein a number of kinds of the cells is at least two with respect to the cell contained container, and wherein a shortest distance between centers of most closely adjacent two concaves of the at least two concaves is 9.0 mm or less. In a preferable mode, the concaves contain a liquid, and a total liquid amount of the liquid with respect to the concaves is 10.0 microliters or less. In a more preferable mode, a filling accuracy in terms of a number in which the cells are contained in the concaves is 30% or lower.