Abstract: Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Abstract: Disclosed herein is an instrument and associated methods for a fully automated bench-top NULISA platform, comprising an X-Y-Z-gantry, a microtiter plate stage, an incubator, a quantitative PCR module, a decontamination cleaner for microtiter plates, a microtiter plate sealer, a magnetic probe and comb assembly for sample mixing and magnetic bead extraction, a storage unit for reagents and supplies, and a controller.

Abstract: Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Abstract: This disclosure relates to a certificate computer system. A rules store stores, for a template identifier, template identifiers of dependency certificates, attribute paths, and functions to compute output certificate attributes. A certificate metadata store stores, for a certificate identifier, a validity state, certificate identifiers of dependency certificates, and a hash value. A processor generates a certificate by receiving a request comprising a template identifier and dependency certificates. The processor queries a rules store to retrieve a template, and computes a value for the attributes by applying a function from the rules store on attribute values of the dependency certificates. The processor creates the output certificate including the computed value for the computed attributes.

Abstract: Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Abstract: A boot verification method is applied to an electronic device having a secure boot function. An algorithm selection identifier is set in the electronic device to indicate an algorithm used by the electronic device in a boot process, so that on the basis of supporting a plurality of algorithms, the electronic device can select, based on the algorithm selection identifier, a corresponding algorithm to perform boot.

Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to nucleic acid are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

Abstract: Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Abstract: The invention relates to methods of multiplex detection of a plurality of target nucleic acids using combinations of labels by contacting a sample comprising a cell comprising a plurality of target nucleic acids with a set of probes, wherein the set of probes comprises subsets of probes comprising a plurality detectable labels that provide unique labeling of each target nucleic acid, wherein each probe subset comprises one or more distinct labels, wherein the number and/or combination of distinct labels is unique for each target nucleic acid; and detecting the detectable labels bound to the respective target nucleic acids. The invention also relates to samples, slides and kits for multiplex detection of target nucleic acids.

Abstract: A method (100) for block generation in a distributed ledger is presented. Each client device of a plurality of client devices (302) to (308) defines an associated battery (200). Each battery (200) comprises a proof-of-work (PoW) (202) and a unique identifier (204) of the associated client device. Each of the plurality of client devices (302) to (308) broadcasts the associated battery (200) to a plurality of nodes that maintain the distributed ledger. The plurality of nodes record the battery in the distributed ledger and select, via a battery selection algorithm, a first battery associated with a first client device to generate a new block in the distributed ledger. The first client device generates the new block using the first battery.

Abstract: A computer implemented method for manipulating data comprises receiving log transaction information from a primary database system at a secondary database system and parsing the log transaction information to identify data objects that have been modified on the primary database system. The secondary database system determines whether the data objects are present in main memory on the secondary database system and whether the log transaction corresponds to a data manipulation language (DML) query or a data definition language (DDL) query. If the data objects are not in the main memory and the query is a DML query, they are copied from persistence data volumes to the main memory. The data objects in main memory are modified by replaying the log transactions on the data objects in the main memory. Then, the data objects in the persistence data volumes are modified. Related apparatus, systems, techniques and articles are also described.

Abstract: Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Abstract: Disclosed herein are highly sensitive immunoassays that utilize a capture/release mechanism to reduce non-specific binding and achieve detection with attomolar-level sensitivity. Kits that can be used for carrying out these highly sensitive immunoassays are also disclosed herein.

Abstract: Restoring of a database table in the database system (e.g., an in-memory insert-only database system, etc.) is initiated. Thereafter, a transaction log volume storing data log records and multi-version concurrency control (MVCC) log records corresponding to the database table is accessed. Based on such access, data log records corresponding to the database table are replayed while insert and update MVCC log records corresponding to the database table are skipped. Subsequently, restoring of the database table in the database system is finalized. Related apparatus, systems, techniques and articles are also described.

Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

Abstract: Disclosed herein are system, method, and computer program product embodiments for a database recovery and optimization with batch processing system. An embodiment operates by retrieving a database log that includes a plurality of operations for modifying data of a database stored across a plurality of tables. From the database log, a plurality of consecutive insert operations for inserting data into the database are identified. The consecutive insert operations are sorted by table. The sorted insert operations are grouped into a batch message. The batch message is transmit to the database for replay. An acknowledgement is received that the replay has completed.

Abstract: A computer implemented method for manipulating data comprises receiving log transaction information from a primary database system at a secondary database system and parsing the log transaction information to identify data objects that have been modified on the primary database system. The secondary database system determines whether the data objects are present in main memory on the secondary database system. If the data objects are not in the main memory, they are copied from persistence data volumes to the main memory. The data objects are modified by replaying the log transactions on the data objects in the main memory. Then, the data objects in the persistence data volumes are modified. Related apparatus, systems, techniques and articles are also described.

Abstract: Disclosed herein are system, method, and computer program product embodiments for a database recovery and optimization with batch processing system. An embodiment operates by retrieving a database log that includes a plurality of operations for modifying data of a database stored across a plurality of tables. From the database log, a plurality of consecutive insert operations for inserting data into the database are identified. The consecutive insert operations are sorted by table. The sorted insert operations are grouped into a batch message. The batch message is transmit to the database for replay. An acknowledgement is received that the replay has completed.