Abstract: The invention discloses an artificial pairing and breeding method of Platysternon megacephalum. The method comprises the selection of species sources, domestication before pairing, setting of a breeding pond, artificial pairing, collection and hatching of turtle eggs. The method fully considers the ecological and biological characteristics of the Platysternon megacephalum, including the behavior, feeding habit, habitat, and breeding ecology. The main problems in artificial pairing and captive breeding of the Platysternon megacephalum can be effectively solved, including the breeding turtle selection, pairing, pairing stage selection, sex ratio, density, artificial food, setting of the reproduction and hatching environments, the occurrence of disabilities and fatal events during the pairing and mating process is significantly reduced; the egg laying rate of female turtles and the hatching rate of turtle eggs are both significantly improved.