Abstract: A method of producing three-dimensional cellular tissues includes mixing stromal cells with a cationic substance, an extracellular matrix component and a polyelectrolyte to obtain a mixture; removing a liquid portion from the mixture to obtain a cell aggregate; culturing the cell aggregate in a medium to obtain a three-dimensional cellular tissue; and culturing the three-dimensional cellular tissue in a medium containing ascorbic acid and Transforming Growth Factor-? (TGF-?), wherein a thickness of the three-dimensional cellular tissue remains greater than 50 ?m for at least 3 days after culturing the three-dimensional cellular tissue in the medium containing ascorbic acid and TGF-?.

Abstract: One aspect of the present invention relates to a production method of a fragmented extracellular matrix component, the method including: a step of neutralizing a solution in which an extracellular matrix component is dissolved; a step of obtaining a mass containing the extracellular matrix component by removing the solvent through freeze-drying treatment after the neutralization step; a step of obtaining a liquid containing the fragmented extracellular matrix component by dispersing the mass in a solution containing a polar organic solvent and fragmenting the extracellular matrix component in the solution; and a step of removing a liquid component from the liquid containing the fragmented extracellular matrix component.

Abstract: A method for evaluating migration ability of migratory cells includes culturing a cell structure having a stromal cell layer containing migratory and stromal cells, and a migratory cell-free cell layer containing no migratory cells, and evaluating migration ability of the migratory cells based on mobility of the migratory cells. The mobility is a ratio of height Hm of the migratory cells to height Hs of the cell structure, the height Hm is distance between point Pb and the migratory cells, the point Pb is intersection where a perpendicular line down from the migratory cells to bottom of the cell structure intersects the bottom of the cell structure, the height Hs is distance between the point Pb and point Pt, the point Pt is an intersection where a straight line obtained by extending the perpendicular line to top surface of the cell structure intersects the top surface of the cell structure.

Abstract: A method of producing three-dimensional cellular tissues includes: obtaining a stromal cell-containing mixture containing stromal cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a stromal cell-containing mixture containing stromal cells, a cationic substance and a fragmented extracellular matrix component; gelling the stromal cell-containing mixture to obtain a first gel composition containing stromal cells; obtaining a target cell-containing mixture containing target cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a target cell-containing mixture containing target cells, a cationic substance and a fragmented extracellular matrix component; placing the target cell-containing mixture in contact with the first gel composition; gelling the target cell-containing mixture to obtain a second gel composition containing target cells; and incubating the first gel composition and the second gel composition to obtain three-dimensional cell

Abstract: A method for producing a three-dimensional tissue construct comprising mature adipocytes, comprising incubating cells comprising at least fat-derived stein cells in the presence of one or more fatty acids selected from the group consisting of erucic acid, elaidic acid, oleic acid, palmitoleic acid, myristoleic acid, phytanic acid, and pristanic acid.

Abstract: A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.

Abstract: The present invention relates to a method for freezing a cell structure including freezing a cell structure including fragmented extracellular matrix components, cells and fibrin and having a three-dimensional tissue structure.

Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.

Abstract: A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.

Abstract: A method of producing a three-dimensional cell tissue include obtaining a mixture including cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, gelling the mixture such that a gel composition including the cells, cationic substance, extracellular matrix component and polyelectrolyte is obtained; and incubating the gel composition such that a three-dimensional cell tissue is obtained.

Abstract: A method of detecting somatic mutations of tumor marker genes includes recovering a peripheral blood mononuclear cell layer from a blood sample collected from a subject and examining whether or not a nucleic acid derived from tumor marker genes having somatic mutations is detected from DNA included in the peripheral blood mononuclear cell layer.

Abstract: The present invention relates to a gel composition containing at least one selected from the group consisting of an extracellular matrix component and a fragmented extracellular matrix component, and an ion of a metal element.

Abstract: A method of producing a conditioned medium for culturing a patient-derived cancer cell, including culturing, in a first medium for 24 hours or longer, a three-dimensional cell tissue including an extracellular matrix component, a polymer electrolyte, and a cell cluster including a fibroblast, and collecting the first medium in which the three-dimensional cell tissue has been cultured as the conditioned medium.

Abstract: A method for producing a three-dimensional cell structure having a vascular network, including preparing a mixture of a cationic substance, a polyelectrolyte, an extracellular matrix component, and a cell population including endothelial cells, collecting, from the mixture, a cell aggregate including the cell population, the cationic substance, the polyelectrolyte, and the extracellular matrix component, and culturing a collected cell aggregate in a medium. The mixture includes the extracellular matrix at a concentration of 1.0×10?8 mg/mL or more and less than 2.5×10?2 mg/mL.

Abstract: A method for producing a three-dimensional cell structure includes preparing a mixture of a cationic substance, an extracellular matrix component, a polyelectrolyte, and a cell population including endothelial cells and mouse-derived stromal cells, which exclude mouse-derived endothelial cells, collecting a cell aggregate from the mixture, and culturing a collected cell aggregate to obtain a three-dimensional cell structure.

Abstract: An evaluation device for evaluating an anticancer effect includes a learning information acquisition unit that acquires learning data that includes state information which indicates at least a cancer state in an unspecified subject and training data that is information about an effect of an anticancer agent obtained by administering the anticancer agent to cells collected from the subject, a learning unit that generates a prediction model by causing a learning model to perform supervised learning for a corresponding relationship between the learning data and the training data, and a prediction unit that makes related predictions to therapy using the anticancer agent with the prediction model. The learning information acquisition unit acquires the information about the anticancer effect obtained by administering the anticancer agent to a three-dimensional cell structure including cancer cells collected from the unspecified subject and cells constituting a stroma as the training data.

Abstract: Disclosed is a method for controlling the Young’s modulus of a three-dimensional tissue containing cells and an extracellular matrix by adjusting the average diameter of an extracellular matrix in production of the three-dimensional tissue.

Abstract: The present invention relates to a cell structure including cells containing at least hepatocytes and vascular endothelial cells, and extracellular matrix component, wherein the extracellular matrix components are disposed between the cells, and a liver sinusoidal network is provided between the cells.

Abstract: A method of assessing a possibility of cancerization including culturing a cell structure including normal cells and having a vascular network structure in a presence of a biological specimen from a subject, and assessing a possibility of cancerization in the subject based on a state of vessels in the cell structure after the culturing. The biological specimen is a body fluid specimen from the subject, a cell extract of cells from the subject, or a culture supernatant of cells from the subject, and the possibility of cancerization is assessed as high in the subject when a number of cells forming the vessels in the cell structure is larger than a number of cells cultured in an absence of the body fluid specimen, or when the vascular network structure in the cell structure extends.

Abstract: Provided is a cell structure including: a connective tissue structure; and an epithelial structure placed on the connective tissue structure, in which the connective tissue structure contains a fragmented extracellular matrix component and first cells including mesenchymal cells, at least a part of the fragmented extracellular matrix component is placed between the first cells, and the epithelial structure contains epithelial cells.