Abstract: Disclosed is an artificial adipose tissue comprising a three-dimensional tissue comprising: a population of cells including an adipocyte; and a fragmented extracellular matrix.

Abstract: The present disclosure relates to a biological tissue model including two or more tissue pieces including a bioink printed material, and having a structure in which the tissue pieces adhere to each other, wherein the bioink contains fragmented extracellular matrix components.

Abstract: A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.

Abstract: A method for testing an anticancer effect including culturing a cell structure including cancer cells and stromal cells in a presence of an anticancer drug and immune cells, and measuring the number of living cancer cells in the cell structure after the culturing, as an indicator of an anticancer effect of the anticancer drug or the immune cells.

Abstract: A first acquisition unit configured to acquire cell feature data indicating a feature of a training cell that is a cell to be a cell tissue having a vascular network structure based on a cell image obtained by imaging the training cell, a second acquisition unit configured to acquire tissue feature data indicating a feature of a training tissue that is a cell tissue produced by culturing the training cell, and a generation unit configured to generate correspondence data in which the cell feature data and the tissue feature data are associated with each other are provided.

Abstract: A method for testing an anticancer effect including culturing a cell structure including cancer cells and stromal cells in a presence of an anticancer drug and immune cells, and measuring the number of living cancer cells in the cell structure after the culturing, as an indicator of an anticancer effect of the anticancer drug or the immune cells.

Abstract: The cell-containing vessel includes a cell culture vessel, a cell culture medium contained in the cell culture vessel, a first cell layer formed on a cell culture surface of the cell culture vessel, and a second cell layer that contains stroma-forming cells and is formed on the first cell layer. The second cell layer is removable from the first cell layer.

Abstract: Disclosed is a method for producing a tissue construct comprising vascular cells, comprising incubating cells comprising at least fat-derived stem cells in the presence of horse serum.

Abstract: An anticancer drug assessment method includes culturing a cell structure including stromal cells in which a gene encoding a live cell marker protein has been knocked out and cancer cells in the presence of an anticancer drug, and assessing the anticancer drug using an expression level of the live cell marker protein as an index in this order. In the assessment, the expression level of the live cell marker protein is measured using a culture supernatant, or in the assessment, a substance that fluoresces or produces color when reacted with the live cell marker protein is added, and the expression level of the live cell marker protein is measured based on the amount of fluorescence or coloration of the substance.

Abstract: The present invention relates to a bioink containing a fragmented extracellular matrix component.

Abstract: The present invention relates to an extracellular matrix-containing composition containing a fragmented extracellular matrix component and a compound bound or adsorbed to the fragmented extracellular matrix component.

Abstract: The present invention relates to an abnormal cardiac rhythm myocardial model composed of a three-dimensional tissue containing cells including the cardiomyocytes and collagen, wherein at least a portion of the cells adheres to the collagen.

Abstract: This method for culturing a cancer organoid includes culturing the cancer organoid on a top surface or inside of a cell structure in a medium not including an extracellular matrix, and the cell structure includes cells constituting stroma, and has two or more cell layers laminated in a thickness direction. A method for screening a test substance includes culturing a cell structure including the cancer organoid obtained by the method for culturing a cancer organoid in a presence of the test substance, and evaluating an effect of the test substance on the cancer organoid.

Abstract: The present invention relates to a method for changing a hydrogel volume, the method including a step A of bringing an ionic polymer into contact with a hydrogel, which has a site having decomposition activity for the ionic polymer and a cross-linkable functional group that is capable of cross-linking with the ionic polymer through electrostatic interaction, to allow the cross-linkable functional group to cross-link with the ionic polymer, and reducing a volume of the hydrogel; and a step B of decomposing the ionic polymer cross-linking with the hydrogel by using the site having decomposition activity and discharging at least part of a decomposition product of the ionic polymer from the hydrogel to increase the hydrogel volume.

Abstract: There is provided a method for producing a tissue construct comprising vascular endothelial cells, the method comprising incubating cells comprising at least adipose-derived stem cells from human in the presence of a TGF? type I receptor inhibitor.

Abstract: A method of producing three-dimensional cellular tissues includes mixing stromal cells with a cationic substance, an extracellular matrix component and a polyelectrolyte to obtain a mixture; removing a liquid portion from the mixture to obtain a cell aggregate; culturing the cell aggregate in a medium to obtain a three-dimensional cellular tissue; and culturing the three-dimensional cellular tissue in a medium containing ascorbic acid and Transforming Growth Factor-? (TGF-?), wherein a thickness of the three-dimensional cellular tissue remains greater than 50 ?m for at least 3 days after culturing the three-dimensional cellular tissue in the medium containing ascorbic acid and TGF-?.

Abstract: One aspect of the present invention relates to a production method of a fragmented extracellular matrix component, the method including: a step of neutralizing a solution in which an extracellular matrix component is dissolved; a step of obtaining a mass containing the extracellular matrix component by removing the solvent through freeze-drying treatment after the neutralization step; a step of obtaining a liquid containing the fragmented extracellular matrix component by dispersing the mass in a solution containing a polar organic solvent and fragmenting the extracellular matrix component in the solution; and a step of removing a liquid component from the liquid containing the fragmented extracellular matrix component.

Abstract: A method for evaluating migration ability of migratory cells includes culturing a cell structure having a stromal cell layer containing migratory and stromal cells, and a migratory cell-free cell layer containing no migratory cells, and evaluating migration ability of the migratory cells based on mobility of the migratory cells. The mobility is a ratio of height Hm of the migratory cells to height Hs of the cell structure, the height Hm is distance between point Pb and the migratory cells, the point Pb is intersection where a perpendicular line down from the migratory cells to bottom of the cell structure intersects the bottom of the cell structure, the height Hs is distance between the point Pb and point Pt, the point Pt is an intersection where a straight line obtained by extending the perpendicular line to top surface of the cell structure intersects the top surface of the cell structure.

Abstract: A method of producing three-dimensional cellular tissues includes: obtaining a stromal cell-containing mixture containing stromal cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a stromal cell-containing mixture containing stromal cells, a cationic substance and a fragmented extracellular matrix component; gelling the stromal cell-containing mixture to obtain a first gel composition containing stromal cells; obtaining a target cell-containing mixture containing target cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, or a target cell-containing mixture containing target cells, a cationic substance and a fragmented extracellular matrix component; placing the target cell-containing mixture in contact with the first gel composition; gelling the target cell-containing mixture to obtain a second gel composition containing target cells; and incubating the first gel composition and the second gel composition to obtain three-dimensional cell

Abstract: A method for producing a three-dimensional tissue construct comprising mature adipocytes, comprising incubating cells comprising at least fat-derived stein cells in the presence of one or more fatty acids selected from the group consisting of erucic acid, elaidic acid, oleic acid, palmitoleic acid, myristoleic acid, phytanic acid, and pristanic acid.