Abstract: An additive manufacturing method for fabricating 3D nanostructures is provided, charged species dispersed in a fluid are precisely arranged at nanoscale in each dimension with a configured electric field, so that the charged species are printed on a substrate to form an array of 3D nanostructures as desired. The additive manufacturing method of the present disclosure can be carried out at room temperature and atmospheric pressure without the aid of chemical reaction, laser sources, ion/electron beams, or photosensitive materials, and enables low-cost, ultra-fast printing speed, large-area, high-purity, multi-material, ultra-high-resolution and solves problems encountered in other nanofabrication techniques in making 3D nanostructures, such as a limited range of available printing materials, low resolution, slow printing speed, and one by one serial printing.

Abstract: Methods for treating subjects who have autoimmune diseases including multiple sclerosis. The methods include administering, e.g., orally, one or more micro RNAs, e.g., miR-30d, miR-7706, and miR-1246, or mimics thereof.