Patents by Inventor Shiva Razavi

Shiva Razavi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11951210
    Abstract: This application is directed to methods which allow for encapsulation of an array of biological materials under physiological conditions that are relevant given a biological context and the compositions made using those methods. These reconstituted biological materials encompass: a) purified proteins that can bind to the lipid membranes inside and outside of vesicles based on the electrostatic charge; b) purified cytosolic proteins that position themselves in the lumen of the vesicles, c) mammalian cell extracts with an array of cytosolic protein content, among other contents and d) small biological molecules such as DNA and RNA as well as fluorescent dyes/probes. These vesicles can be used to simulate cells in drug discovery methods as well as useful in administering drugs and other compositions to cells in vitro and in vivo.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: April 9, 2024
    Assignee: THE JOHNS HOPKINS UNIVERSTY
    Inventors: Shiva Razavi, Takanari Inoue, Tianzhi Luo, Douglas Robinson
  • Publication number: 20240067957
    Abstract: Genetic circuits that control transgene expression in response to pre-defined transcriptional cues would enable the development of smart therapeutics. The present disclosure relates to engineered programmable single-transcript RNA sensors in which adenosine deaminases acting on RNA (ADARs) autocatalytically convert trigger hybridization into a translational output. This system amplifies the signal from editing by endogenous ADAR through a positive feedback loop. Amplification is mediated by the expression of a hyperactive, minimal ADAR variant and its recruitment to the edit site via an orthogonal RNA targeting mechanism. This topology confers high dynamic range, low background, minimal off-target effects, and a small genetic footprint. The circuits and systems disclosed herein leverage an ability to detect single nucleotide polymorphisms and modulate translation in response to endogenous transcript levels in mammalian cells.
    Type: Application
    Filed: June 22, 2023
    Publication date: February 29, 2024
    Applicants: Massachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: James J. Collins, Raphael Gayet, Katherine IIia, Shiva Razavi, Nathaniel Tippens, Kehan Zhang, Jack Chen, Jonathan Chen, Makoto Lalwani
  • Publication number: 20210145746
    Abstract: This application is directed to methods which allow for encapsulation of an array of biological materials under physiological conditions that are relevant given a biological context and the compositions made using those methods. These reconstituted biological materials encompass: a) purified proteins that can bind to the lipid membranes inside and outside of vesicles based on the electrostatic charge; b) purified cytosolic proteins that position themselves in the lumen of the vesicles, c) mammalian cell extracts with an array of cytosolic protein content, among other contents and d) small biological molecules such as DNA and RNA as well as fluorescent dyes/probes. These vesicles can be used to simulate cells in drug discovery methods as well as useful in administering drugs and other compositions to cells in vitro and in vivo.
    Type: Application
    Filed: December 12, 2018
    Publication date: May 20, 2021
    Inventors: Shiva Razavi, Takanari Inoue, Tianzhi Luo, Douglas Robinson