Abstract: Seeds of alloplasmic wheat, which contain at least one specific nuclear gene (Ms) and a cytoplasm which interacts with the specific nuclear gene (Ms) or genes (Ms Ms) to maintain the plant fertile, are treated by a mutagenic agent and, if necessary selfed to obtain seeds having embryos that contain only mutated nuclear genes (ms ms). The seeds having embryos without at least one specific nuclear gene (Ms), produce plants which are male sterile (A-line). The A-line plants are pollinated with pollen from euplasmic or alloplasmic wheat (R-line), having chromosomes including specific nuclear genes (Ms Ms), to produce hybrid wheat seeds. The available A-line seeds may be multiplied by crossing the A-line plants with B-line plants to produce A-line seeds.