Patents by Inventor Shizu Takeda
Shizu Takeda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11926845Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: GrantFiled: January 29, 2021Date of Patent: March 12, 2024Assignee: Hitachi, Ltd.Inventors: Yuki Niimi, Hiroko Hanzawa, Maiko Tanabe, Kunio Ohyama, Shizu Takeda
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Patent number: 11566232Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.Type: GrantFiled: September 6, 2018Date of Patent: January 31, 2023Assignees: HITACHI, LTD., THE UNIVERSITY OF TOKYOInventors: Maiko Tanabe, Shizu Takeda, Masahiro Okanojo, Hiroko Hanzawa, Masao Washizu, Kennedy Omondi Okeyo
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Publication number: 20210301248Abstract: Provided are an evaluator including an analysis unit that evaluates a differentiation level of a cultured cell on the basis of a content of a component in a culture supernatant of the cultured cell, and an automatic cell culture system including the evaluator and an automatic culture device.Type: ApplicationFiled: November 18, 2020Publication date: September 30, 2021Inventors: Hikaru SAITO, Midori KATO, Kakuro HIRAI, Masaharu KIYAMA, Kunio OHYAMA, Hiroko HANZAWA, Shizu TAKEDA
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Publication number: 20210284959Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: ApplicationFiled: January 29, 2021Publication date: September 16, 2021Inventors: Yuki NIIMI, Hiroko HANZAWA, Maiko TANABE, Kunio OHYAMA, Shizu TAKEDA
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Patent number: 10947490Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.Type: GrantFiled: February 14, 2018Date of Patent: March 16, 2021Assignee: HITACHI, LTD.Inventors: Hikaru Saito, Masaharu Kiyama, Midori Kato, Kunio Ohyama, Guangbin Zhou, Hiroko Hanzawa, Shizu Takeda
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Publication number: 20210009935Abstract: An object of the invention is to provide a cell culture monitoring device capable of monitoring the number of cells during culture and a cell culture system. The cell culture monitoring device for monitoring proliferation of cells includes: a detection unit configured to detect particles of exosomes in culture supernatant; and an analysis unit configured to calculate the number of cells based on an obtained detection result. The cell culture system includes the cell culture monitoring device and an automatic culture device.Type: ApplicationFiled: July 9, 2020Publication date: January 14, 2021Inventors: Hikaru SAITO, Kakuro HIRAI, Midori KATO, Masaharu KIYAMA, Kunio OHYAMA, Hiroko HANZAWA, Shizu TAKEDA
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Publication number: 20200327958Abstract: A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.Type: ApplicationFiled: March 19, 2020Publication date: October 15, 2020Inventors: Maiko TANABE, Shizu TAKEDA, Kiyoto ITO, Osamu IMAICHI, Kenji TSUGE, Michihiro ARAKI
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Patent number: 10329525Abstract: There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipe 7 is connected with a gas bag 14, the liquid to be fed into the feed pipe 7 is fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a container 8 for holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container.Type: GrantFiled: July 23, 2014Date of Patent: June 25, 2019Assignee: Hitachi, Ltd.Inventors: Masaharu Kiyama, Guangbin Zhou, Takayuki Nozaki, Ryota Nakajima, Daisuke Suzuki, Yumiko Igarashi, Shizu Takeda, Taku Nakamura, Masakazu Sugaya, Koichi Terada
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Publication number: 20190085299Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.Type: ApplicationFiled: September 6, 2018Publication date: March 21, 2019Inventors: Maiko TANABE, Shizu TAKEDA, Masahiro OKANOJO, Hiroko HANZAWA, Masao WASHIZU, Kennedy Omondi OKEYO
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Publication number: 20180362915Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.Type: ApplicationFiled: February 14, 2018Publication date: December 20, 2018Inventors: Hikaru SAITO, Masaharu KIYAMA, Midori KATO, Kunio OHYAMA, Guangbin ZHOU, Hiroko HANZAWA, Shizu TAKEDA
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Patent number: 10087410Abstract: In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610, and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.Type: GrantFiled: March 25, 2013Date of Patent: October 2, 2018Assignee: Hitachi, Ltd.Inventors: Takayuki Nozaki, Guangbin Zhou, Masaharu Kiyama, Taku Nakamura, Shizu Takeda, Ryota Nakajima, Masakazu Sugaya, Koichi Terada
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Patent number: 9862921Abstract: There is provided a method for noninvasively evaluating the cell state (proliferation, multi-layering, and differentiation) of a cell sheet as a mimic tissue at the time of culturing the cell sheet. The method is characterized in that an analysis of an amino acid is conducted with the use of the culture supernatant of a cell sheet to monitor a change in the concentration of any amino acid selected from a group of 5-species of amino acids (Ile, Val, Ser, Leu, and Ala), thereby making a determination.Type: GrantFiled: June 17, 2014Date of Patent: January 9, 2018Assignee: Hitachi High-Technologies CorporationInventors: Naoko Senda, Yoko Inoue, Hiroaki Nakagawa, Akihiro Shimase, Ryota Nakajima, Kazumichi Imai, Shizu Takeda
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Publication number: 20170198249Abstract: There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipe 7 is connected with a gas bag 14, the liquid to be fed into the feed pipe 7 is fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a container 8 for holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container.Type: ApplicationFiled: July 23, 2014Publication date: July 13, 2017Inventors: Masaharu KIYAMA, Guangbin ZHOU, Takayuki NOZAKI, Ryota NAKAJIMA, Daisuke SUZUKI, Yumiko IGARASHI, Shizu TAKEDA, Taku NAKAMURA, Masakazu SUGAYA, Koichi TERADA
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Publication number: 20170145365Abstract: A culture which maintains an aseptic state at the time of culturing is achieved, the avoidance of damage to a closed-system culture vessel and of an influence to the cells due to impacts and oscillation is possible during transportation after being manufactured. The closed-system culture vessel 101 has culture vessels 201, 202 for maintaining the cells and/or the culture medium, and a culturing space which is closed by holding members 203, 204 for holding the culture vessels, and the holding member has a semi-open cavity 221 independent of the culturing space. Further, the holding member, by being provided with a lid fixing ring 220 surrounding a connector part 214, is not damaged even when impacts are applied during transportation.Type: ApplicationFiled: July 29, 2014Publication date: May 25, 2017Inventors: Takayuki NOZAKI, Masaharu KIYAMA, Masakazu SUGAYA, Guangbin ZHOU, Taku NAKAMURA, Daisuke SUZUKI, Shizu TAKEDA, Yumiko IGARASHI, Koichi TERADA
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Publication number: 20160122702Abstract: There is provided a method for noninvasively evaluating the cell state (proliferation, multi-layering, and differentiation) of a cell sheet as a mimic tissue at the time of culturing the cell sheet. The method is characterized in that an analysis of an amino acid is conducted with the use of the culture supernatant of a cell sheet to monitor a change in the concentration of any amino acid selected from a group of 5-species of amino acids (Ile, Val, Ser, Leu, and Ala), thereby making a determination.Type: ApplicationFiled: June 17, 2014Publication date: May 5, 2016Inventors: Naoko SENDA, Yoko INOUE, Hiroaki NAKAGAWA, Akihiro SHIMASE, Ryota NAKAJIMA, Kazumichi IMAI, Shizu TAKEDA
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Publication number: 20160017271Abstract: In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610, and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.Type: ApplicationFiled: March 25, 2013Publication date: January 21, 2016Inventors: Takayuki NOZAKI, Guangbin ZHOU, Masaharu KIYAMA, Taku NAKAMURA, Shizu TAKEDA, Ryota NAKAJIMA, Masakazu SUGAYA, Koichi TERADA
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Publication number: 20050100966Abstract: Protein complexes are provided comprising FHOS and one or more proteins selected from the group consisting of GROUP1. Methods of using the protein complexes in diagnosing diseases and disorders are also provided. In addition, the protein complexes are also useful in screening assays for identifying compounds effective in treating and/or preventing diseases and disorders associated with FHOS and its interactors.Type: ApplicationFiled: March 19, 2004Publication date: May 12, 2005Inventors: Takeshi Sakamoto, Shizu Takeda