Patents by Inventor Shizuo Hattori
Shizuo Hattori has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6884416Abstract: The present invention provides a stable lyophilized PQQ-dependent glucose dehydrogenase composition comprising a PQQ-dependent glucose dehydrogenase together with (i) at least one compound selected from the group consisting of aspartic acid, glutamic acid, ?-ketoglutaric acid, malic acid, ?-ketogluconic acid, ?-cyclodextrin and their salts and (ii) an albumin.Type: GrantFiled: February 12, 2001Date of Patent: April 26, 2005Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Atsushi Sogabe, Seiji Takeshima, Yoshihisa Kawamura
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Patent number: 6867012Abstract: The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, and homocysteine, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement.Type: GrantFiled: December 3, 2001Date of Patent: March 15, 2005Assignee: Toyo Boseki Kabushiki KaishaInventors: Takahide Kishimoto, Atsushi Sogabe, Shizuo Hattori, Masanori Oka, Yoshihisa Kawamura
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Publication number: 20020119507Abstract: The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, homocysteine and the like, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement.Type: ApplicationFiled: December 3, 2001Publication date: August 29, 2002Inventors: Takahide Kishimoto, Atsushi Sogabe, Shizuo Hattori, Masanori Oka, Yoshihisa Kawamura
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Publication number: 20020076751Abstract: The invention relates to a plasmid characterized in that the plasmid comprises a DNA fragment containing a gene coding for an enzyme taking PQQ as the prosthetic group as cloned in a broad-host-range vector defected of conjugative transfer function beforehand and that the plasmid is capable of being expressed in bacteria of the genus Pseudomonas.Type: ApplicationFiled: January 18, 2001Publication date: June 20, 2002Inventors: Shizuo Hattori, Seiji Takeshima, Atsushi Sogabe, Yoshihisa Kawamura
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Publication number: 20010021523Abstract: The present invention provides a stable lyophilized PQQ-dependent glucose dehydrogenase composition comprising a PQQ-dependent glucose dehydrogenase together with (i) at least one compound selected from the group consisting of aspartic acid, glutamic acid, &agr;-ketoglutaric acid, malic acid, &agr;-ketogluconic acid, &agr;-cyclodextrin and their salts and (ii) an albumin.Type: ApplicationFiled: February 12, 2001Publication date: September 13, 2001Inventors: Shizuo Hattori, Atsushi Sogabe, Seiji Takeshima, Yoshihisa Kawamura
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Patent number: 5874078Abstract: A novel glutamate dehydrogenase derived from the genus Pseudomonas which is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, is with an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP. A reagent composition for measuring an amount of ammonia characterized in containing a glutamate dehydrogenase which is not activated by ADP and is NADH-dependent, .alpha.-ketoglutaric acid or a salt thereof and NADH; and a reagent composition wherein urease or creatinine deiminase is further added to the above-mentioned reagent composition.Type: GrantFiled: January 4, 1996Date of Patent: February 23, 1999Assignee: Toyo Boseki Kabushiki KaishaInventors: Noriyuki Hongo, Shizuo Hattori, Kazumi Yamamoto, Shinichi Teshima, Yoshihisa Kawamura
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Patent number: 5821061Abstract: A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.Type: GrantFiled: September 6, 1996Date of Patent: October 13, 1998Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Kazumi Yamamoto, Shinichi Teshima, Yoshihisa Kawamura
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Patent number: 5821095Abstract: A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.Type: GrantFiled: January 12, 1996Date of Patent: October 13, 1998Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Kazumi Yamamoto, Shinichi Teshima, Yoshihisa Kawamura
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Patent number: 5773585Abstract: A novel glutamate dehydrogenase is derived from the genus Pseudomonas and is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, has an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP.Type: GrantFiled: June 21, 1996Date of Patent: June 30, 1998Assignee: Toyo Boseki Kabushiki KaishaInventors: Noriyuki Hongo, Shizuo Hattori, Kazumi Yamamoto, Shinichi Teshima, Yoshihisa Kawamura
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Patent number: 5741687Abstract: The sorbitol oxidase of the present invention catalyzes the reaction in which D-sorbitol is oxidized D-glucose and hydrogen peroxide.Type: GrantFiled: June 6, 1995Date of Patent: April 21, 1998Assignee: Toyo Boseki Kaubshiki KaishaInventors: Shizuo Hattori, Yukihiro Sogabe, Shigenori Emi
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Patent number: 5624812Abstract: A method for measuring carbon dioxide, comprising the steps of: (1) reacting bicarbonate ion in a sample with phosphoenolpyruvate carboxylase derived from an acetic acid bacterium in the presence of phosphoenolpyruvate; (2) reacting the resultant oxalacetic acid with malate dehydrogenase in the presence of NADH; and (3) measuring decreased NADH, and a reagent for measuring carbon dioxide, comprising phosphoenolpyruvate, phosphoenolpyruvate carboxylase derived from an acetic acid bacterium, malate dehydrogenase, NADH and a buffer. According to the present invention, a highly stable reagent for CO.sub.2 measurement, which permits a long-term storage in a liquid state, can be provided by the use of phosphoenolpyruvate carboxylase derived from a species of acetic acid bacteria.Type: GrantFiled: June 6, 1995Date of Patent: April 29, 1997Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Takahide Kishimoto, Yukihiro Sogabe, Shigenori Emi
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Patent number: 5607838Abstract: A method for determining .alpha.-amylase activity which involves bringing a sample into contact with an .alpha.-glucosidase in the presence of an .alpha.-amylase substrate and optically determining a liberated label, the substrate being a maltooligosaccharide composed of at least 3 glucose units, whose reducing terminal glucose is bonded to an optically measurable label at the 1-position by .alpha.-glucoside linkage or .beta.-glucoside linkage, and whose non-reducing terminal glucose is modified by a substituent other than glucose, and the .alpha.-glucosidase being substantially capable of acting on glucose to which the label is bonded at the 1-position by .alpha.-glucoside linkage and on all maltooligosaccharides having 2 to 7 glucose units; and a reagent for determining .alpha.-amylase activity comprising the .alpha.-glucosidase and said .alpha.-amylase substrate. The present invention permits, in the determination of .alpha.Type: GrantFiled: May 25, 1994Date of Patent: March 4, 1997Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Yoshihiro Yamamoto, Yukihiro Sogabe, Shigenori Emi
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Patent number: 5547851Abstract: A method for measuring carbon dioxide, comprising the steps of: (1) reacting bicarbonate ion in a sample with phosphoenolpyruvate carboxylase derived from an acetic acid bacterium in the presence of phosphoenolpyruvate; (2) reacting the resultant oxalacetic acid with malate dehydrogenase in the presence of NADH; and (3) measuring decreased NADH, and a reagent for measuring carbon dioxide, comprising phosphoenolpyruvate, phosphoenolpyruvate carboxylase derived from an acetic acid bacterium, malate dehydrogenase, NADH and a buffer. According to the present invention, a highly stable reagent for CO.sub.2 measurement, permitting a long-term storage in a liquid state can be provided by the use of phosphoenolpyruvate carboxylase derived from an acetic acid bacterium.Type: GrantFiled: March 18, 1994Date of Patent: August 20, 1996Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Takahide Kishimoto, Yukihiro Sogabe, Shigenori Emi
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Patent number: 5472862Abstract: An isolated sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512 is disclosed. The oxidase catalyzes the reaction of D-sorbitol+O.sub.2 .fwdarw.D-glucose+H.sub.2 O.sub.2, has a substrate specificity for D-sorbitol, D-mannitol, D-xylitol, and D-arabitol, has an optimum pH of 6.5 to 7.5, and has a molecular weight of 54 kD as determined by gel filtration or 43 kD as determined by SDS-PAGE. The enzyme can be used for measuring a polyol in a sample and as a reagent in a kit used for determining the presence of D-sorbitol.Type: GrantFiled: December 7, 1993Date of Patent: December 5, 1995Assignee: Toyo Boseki Kabushiki KaishaInventors: Shizuo Hattori, Yukihiro Sogabe, Shigenori Emi
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Patent number: 5278297Abstract: A gene coding for Protein H, which is capable of binding specifically to human IgG of all subclasses, was isolated from Streptococcus sp. AP1 and expressed in host cells, E. coli to produce the Protein H.Type: GrantFiled: October 15, 1992Date of Patent: January 11, 1994Assignees: Sumitomo Pharmaceuticals Company, Ltd., HighTech Receptor ABInventors: Hideyuki Gomi, Tatsunobu Hozumi, Shizuo Hattori, Chiaki Tagawa, Fumitaka Kishimoto, Lars Bjorck
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Patent number: 5180810Abstract: A gene coding for Protein H, which is capable of binding specifically to human IgG of all subclasses, was isolated from Streptococcus sp. APl and expressed in host cells, E. coli to produce the Protein H.Type: GrantFiled: July 7, 1989Date of Patent: January 19, 1993Assignees: Sumitomo Chemical Co., Limited, HighTech Receptor ABInventors: Hideyuki Gomi, Tatsunobu Hozumi, Shizuo Hattori, Chiaki Tagawa, Fumitaka Kishimoto, Lars Bjorck