Patents by Inventor Shoichiro Tsukita

Shoichiro Tsukita has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cultured cell lines expressing mutant gene
    Patent number: 7951930
    Abstract: Using protein visualization techniques, cell lines stably expressing GFP-fused full-length and mutant APCs were obtained from cultured Xenopus laevis renal epithelial A6 cells. The use of these cells showed that mutant APCs, whose C-terminal region is absent, induced piling up of cells. Furthermore, piled up cells from mutant APC-expressing cell lines were proven to maintain the intercellular adhesive structure, representing a phenomenon similar to polyp formation in individual organisms (mice).
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: May 31, 2011
    Assignee: Eisai R&D Management Co., Ltd.
    Inventors: Yuko Kiyosue, Hiroyuki Sasaki, Shoichiro Tsukita
  • CULTURED XENOPUS LAEVIS CELL LINES EXPRESSING MUTANT ADENOMATOUS POLYPOSIS COLI GENE
    Publication number: 20100003747
    Abstract: Using protein visualization techniques, cell lines stably expressing GFP-fused full-length and mutant APCs were obtained from cultured Xenopus laevis renal epithelial A6 cells. The use of these cells showed that mutant APCs, whose C-terminal region is absent, induced piling up of cells. Furthermore, piled up cells from mutant APC-expressing cell lines were proven to maintain the intercellular adhesive structure, representing a phenomenon similar to polyp formation in individual organisms (mice).
    Type: Application
    Filed: March 21, 2008
    Publication date: January 7, 2010
    Applicant: Eisai Co., Ltd.
    Inventors: Yuko Kiyosue, Hiroyuki Sasaki, Shoichiro Tsukita
  • Cultured cell lines expressing mutant gene
    Patent number: 7371843
    Abstract: Using protein visualization techniques, cell lines stably expressing GFP-fused full-length and mutant APCs were obtained from cultured Xenopus laevis renal epithelial A6 cells. The use of these cells showed that mutant APCs, whose C-terminal region is absent, induced piling up of cells. Furthermore, piled up cells from mutant APC-expressing cell lines were proven to maintain the intercellular adhesive structure, representing a phenomenon similar to polyp formation in individual organisms (mice).
    Type: Grant
    Filed: August 19, 2003
    Date of Patent: May 13, 2008
    Assignee: Eisai Co., Ltd.
    Inventors: Yuko Kiyosue, Hiroyuki Sasaki, Shoichiro Tsukita
  • Protein polymer having unfold activity on higher-order structure of protein
    Patent number: 7335734
    Abstract: The invention of this application provides a protein polymer of 8 to 15 proteins in association, wherein each protein has the amino acid sequence of SEQ ID NO: 1, and where the protein polymer has an unfold activity on the higher-order structure of a protein and this protein polymer is useful for the development of therapeutic agents of various diseases due to the failure in the formation of the higher-order structure of a protein, and the like.
    Type: Grant
    Filed: May 2, 2002
    Date of Patent: February 26, 2008
    Assignee: Japan Science and Technology Corporation
    Inventors: Naomi Sakasegawa, Hiroyuki Sasaki, Shoichiro Tsukita
  • Cultured xenopus cell line expressing mutated apc
    Publication number: 20060100418
    Abstract: Using protein visualization techniques, cell lines stably expressing GFP-fused full-length and mutant APCs were obtained from cultured Xenopus laevis renal epithelial A6 cells. The use of these cells showed that mutant APCs, whose C-terminal region is absent, induced piling up of cells. Furthermore, piled up cells from mutant APC-expressing cell lines were proven to maintain the intercellular adhesive structure, representing a phenomenon similar to polyp formation in individual organisms (mice).
    Type: Application
    Filed: August 19, 2003
    Publication date: May 11, 2006
    Applicant: Eisai Co., Ltd.
    Inventors: Yuko Kiyosue, Hiroyuki Sasaki, Shoichiro Tsukita
  • Protein polymer having unfold activity on higher-order structure of protein
    Publication number: 20050048078
    Abstract: The invention of this application provides a protein polymer of 8 to 15 proteins in association, each of the proteins having the amino acid sequence SQ ID NO. 1, where the protein polymer has an unfold activity on higher-order structure of protein and this protein polymer is useful for the development of therapeutic agents of various diseases due to the failure in the formation of higher-order structure of protein, and the like.
    Type: Application
    Filed: May 2, 2002
    Publication date: March 3, 2005
    Inventors: Naomi Sakasegawa, Hiroyuki Sasaki, Shoichiro Tsukita
  • Antibodies to occludin proteins
    Patent number: 6559286
    Abstract: The present invention discloses antibodies that bind to mammalian occludin proteins which are transmembrane adhesion molecules of the tight junction. Nucleic acid and amino acid sequences encoding human, mammalian, and murine occludin are utilized to prepare and isolate occludin-specific antibodies. These antibodies may be used to elucidate and regulate the activities of tight junctions.
    Type: Grant
    Filed: November 23, 1998
    Date of Patent: May 6, 2003
    Assignee: Eisai Co., Ltd.
    Inventor: Shoichiro Tsukita
  • Cloned DNA encoding mammalian occludins
    Patent number: 6489460
    Abstract: Whole structures of mammalian analogues of occludin, a constituent protein of the tight junction (TJ), are provided. Genes for human, canine and mouse occludins were analyzed with the PCR technique on the basis of the coding sequence seen around the gene for neuronal apoptosis inhibitory protein. With antibodies prepared, the occludins have been confirmed to be constituent proteins of the TJ by immunofluorescent cell staining.
    Type: Grant
    Filed: November 5, 1997
    Date of Patent: December 3, 2002
    Assignee: Eisai Company, Ltd.
    Inventor: Shoichiro Tsukita