Abstract: Provided are compositions and methods for transducing immune cells in vivo where a viral particle comprising a polynucleotide encoding a chimeric antigen receptor and a multipartite cell-surface receptor is administered to a subject.

Abstract: Provided are compositions and methods for transducing immune cells in vivo where a multispecific antibody (e.g. a bispecific T-cell engager) is administered to render immune cells in the subject more transducible by a vector, such as a lentiviral vector.

Abstract: The present disclosure provides polypeptides, compositions thereof, and methods for suppressing expression of a target gene such as PDCD1, CTLA4, LAG3, or TIM-3. The polypeptides disclosed herein include a DNA binding domain (DBD) that binds to a sequence of the target gene and a transcriptional repressor domain that suppresses expression of the target gene. The transcriptional repressor domain may be a known transcriptional repressor or may be a novel transcriptional repressor disclosed herein. Also disclosed herein are novel transcriptional repressors that are conjugated to a heterologous DNA binding domain and mediate suppression of expression of a target gene bound by the DNA binding domain.