Abstract: A tool storage box with sliding and rotating functions includes a box body, a sliding base, and a tray for tool head portions. The box body has a space for the sliding base and the tray. The sliding base controls sliding of the tray in the space. The sliding base is provided with a pivotal shaft and a locking hook for controlling the sliding base. The pivotal hole has a pivotal trough. A peripheral surface of the pivotal trough has a positioning surface. The tray has a pivotal shaft and a positioning post for controlling rotation of the tray. The pivotal shaft engages with the pivotal hole of the sliding base to act as a center of rotation of the tray. When the tray slides out of the box body, the positioning post engages with the pivotal trough, and the tray is rotatable by means of an external force.