Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

Abstract: A low-oxygen heating-type cigarette assembly, comprising a low-oxygen heating-type smoking set (B) and a low-oxygen heating-type cigarette (A). The low-oxygen heating-type smoking set (B) comprises a heating section (4) and a smoke extracting member (2) detachably arranged with the heating section (4), wherein the heating section (4) is provided with a cigarette receiving chamber (4-1), and the smoke extracting member (2) comprises a hollow smoke extracting cone (2-1) and a smoke transmission tube (2-2).

Abstract: Provided herein are recombinant Aspergillus niger capable of producing 3-hydroxypropionic acid (3-HP). Also provided are methods of producing 3-hydroxypropionic acid (3-HP) and related kits.

Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

Abstract: A microcrystalline glass containing a water-repellent and oil-repellent composite coating layer on the surface, a preparation method and application thereof. The coated glass is a microcrystalline glass or a glass ceramic containing a water-repellent and oil-repellent composite coating layer on the surface, which is characterized in that from the outmost surface of the glass, it includes: a water-repellent and oil-repellent layer, an intermediate layer, a bottom layer and a microcrystalline glass or a glass ceramic, wherein, the intermediate layer is the intermediate layer containing ionic crystals with a lattice energy of 700-3000 kJ/mol and formed by the same, the bottom layer includes compounds containing Si—O bonds or a mixed silicon oxide layer.

Abstract: Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).

Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.

Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.

Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.

Abstract: A data link layer switch includes a switching mechanism coupled to a plurality of port interface controllers. Each of the port interface controllers comprises a multicast address table, a multicast matcher, and a timer. The multicast address table stores multicast addresses for hosts attached to the port interface controller. The multicast matcher matches an incoming packet to a target pattern, generates a multicast address from the incoming packet if the incoming packet matches the target pattern, and stores the generated multicast address in the multicast address table. The timer determines an amount of time that the generated multicast address remains in the multicast address table.

Abstract: A data link layer switch includes a switching mechanism coupled to a plurality of port interface controllers. Each of the port interface controllers comprises a multicast address table, a multicast matcher, and a timer. The multicast address table stores multicast addresses for hosts attached to the port interface controller. The multicast matcher matches an incoming packet to a target pattern, generates a multicast address from the incoming packet if the incoming packet matches the target pattern, and stores the generated multicast address in the multicast address table. The timer determines an amount of time that the generated multicast address remains in the multicast address table.

Abstract: A data link layer switch includes a switching mechanism coupled to a plurality of port interface controllers. Each of the port interface controllers comprises a multicast address table, a multicast matcher, and a timer. The multicast address table stores multicast addresses for hosts attached to the port interface controller. The multicast matcher matches an incoming packet to a target pattern, generates a multicast address from the incoming packet if the incoming packet matches the target pattern, and stores the generated multicast address in the multicast address table. The timer determines an amount of time that the generated multicast address remains in the multicast address table.

Abstract: A data link layer switch includes a switching mechanism coupled to a plurality of port interface controllers. Each of the port interface controllers comprises a multicast address table, a multicast matcher, and a timer. The multicast address table stores multicast addresses for hosts attached to the port interface controller. The multicast matcher matches an incoming packet to a target pattern, generates a multicast address from the incoming packet if the incoming packet matches the target pattern, and stores the generated multicast address in the multicast address table. The timer determines an amount of time that the generated multicast address remains in the multicast address table.

Abstract: A data link layer switch includes a switching mechanism coupled to a plurality of port interface controllers. Each of the port interface controllers comprises a multicast address table, a multicast matcher, and a timer. The multicast address table stores multicast addresses for hosts attached to the port interface controller. The multicast matcher matches an incoming packet to a target pattern, generates a multicast address from the incoming packet if the incoming packet matches the target pattern, and stores the generated multicast address in the multicast address table. The timer determines an amount of time that the generated multicast address remains in the multicast address table.

Abstract: A system and method for establishing voice communication between a packet-switched network and a circuit-switched network includes a network interface unit for coupling to the packet-switched network, a phone interface unit for coupling to the circuit-switched network and a gateway processor coupled to the network interface unit and to the phone interface unit. In response to a call request, a packet-switched network connection is established between the gateway processor and a first station in the packet-switched network, and a circuit-switched network-connection is established between the gateway processor and a second station in the circuit-switched network. Incoming voice data packets received from the first station are converted to outgoing voice signals, which are transmitted over the circuit-switched network connection to the second station.

Abstract: A system and method for establishing voice communication between a packet-switched network and a circuit-switched network includes a network interface unit for coupling to the packet-switched network, a phone interface unit for coupling to the circuit-switched network and a gateway processor coupled to the network interface unit and to the phone interface unit. In response to a call request, a packet-switched network connection is established between the gateway processor and a first station in the packet-switched network, and a circuit-switched network-connection is established between the gateway processor and a second station in the circuit-switched network. Incoming voice data packets received from the first station are converted to outgoing voice signals, which are transmitted over the circuit-switched network connection to the second station.

Abstract: An Ethernet ADSL adapter controls data communication between an Ethernet port and an ADSL modem connected to an ADSL channel. The adapter includes a first buffer for storing data packets received at the Ethernet port, a second buffer for storing data packets received from the ADSL modem and a controller. When the first buffer contains a first predetermined number of data packets and data packets are not available for transmission to the Ethernet port, a jamming signal is transmitted from the Ethernet port. The jamming signal inhibits transmission of additional data packets to the Ethernet port by other Ethernet nodes until space is available in the first buffer. When the second buffer contains a second predetermined number of data packets, a pause signal is transmitted to the ADSL modem. The pause signal inhibits further transmission of data packets to the ADSL modem on the ADSL channel until space is available in the second+ buffer.

Abstract: A system and method for establishing voice communication between a packet-switched network and a circuit-switched network includes a network interface unit for coupling to the packet-switched network, a phone interface unit for coupling to the circuit-switched network and a gateway processor coupled to the network interface unit and to the phone interface unit. In response to a call request, a packet-switched network connection is established between the gateway processor and a first station in the packet-switched network, and a circuit-switched network connection is established between the gateway processor and a second station in the circuit-switched network. Incoming voice data packets received from the first station are converted to outgoing voice signals, which are transmitted over the circuit-switched network connection to the second station.