Patents by Inventor Shuangfei Li

Shuangfei Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for preparing a sterile transgenic carp
    Patent number: 7230156
    Abstract: The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (?-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3? non-coding sequence, and 3? flanking sequence of grass carp growth hormone gene as a stop sequence.
    Type: Grant
    Filed: March 31, 2005
    Date of Patent: June 12, 2007
    Assignee: Institute of Hydrobiology, Chinese Academy of Sciences
    Inventors: Wei Hu, Zuoyan Zhu, Yaping Wang, Shuangfei Li
  • Method for preparing a sterile transgenic fish
    Publication number: 20050257281
    Abstract: The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (?-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3? non-coding sequence, and 3? flanking sequence of grass carp growth hormone gene as a stop sequence.
    Type: Application
    Filed: March 31, 2005
    Publication date: November 17, 2005
    Inventors: Wei Hu, Zuoyan Zhu, Yaping Wang, Shuangfei Li