Abstract: A technology is described for one-time access to a protected resource. In one example, a resource security API (Application Programming Interface) may receive a request for a protected resource. The request may be associated with a launch of a software container in a service provider environment to host a process associated with a customer account. The resource security API may identify a single-use token linked to the software container that allows the resource security API to provide the protected resource. The resource security API may send the protected resource to the software container in response to the request when the single-use token is identified, and the resource security API may disable the single-use token linked to the software container to prevent additional requests associated with the software container from accessing the protected resource.