Patents by Inventor Shuhei Hashiro

Shuhei Hashiro has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11884951
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Grant
    Filed: May 21, 2021
    Date of Patent: January 30, 2024
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Publication number: 20210269840
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Application
    Filed: May 21, 2021
    Publication date: September 2, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Patent number: 11046986
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Grant
    Filed: September 25, 2019
    Date of Patent: June 29, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Patent number: 10941390
    Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
    Type: Grant
    Filed: June 10, 2020
    Date of Patent: March 9, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Noriko Yokoyama, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
  • Publication number: 20210024931
    Abstract: A method for efficiently inducing RNA silencing in a target organism is provided. RNA silencing is induced in a target organism by allowing the target organism to ingest cells of a microorganism, wherein the microorganism is able to produce RNA that induces RNA silencing after treating the cells with an organic solvent.
    Type: Application
    Filed: August 14, 2020
    Publication date: January 28, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Nakano, Teruyuki Niimi, Haruka Kawaguchi
  • Publication number: 20200318093
    Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
    Type: Application
    Filed: June 10, 2020
    Publication date: October 8, 2020
    Applicant: AJINOMOTO CO., INC.
    Inventors: Noriko YOKOYAMA, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
  • Patent number: 10717971
    Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: July 21, 2020
    Assignee: AJINOMOTO CO., LTD.
    Inventors: Noriko Yokoyama, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
  • Publication number: 20200024629
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Application
    Filed: September 25, 2019
    Publication date: January 23, 2020
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Publication number: 20170044513
    Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
    Type: Application
    Filed: August 31, 2016
    Publication date: February 16, 2017
    Applicant: AJINOMOTO CO., INC.
    Inventors: Noriko YOKOYAMA, Mayu NAKANO, Shuhei HASHIRO, Masako HIRAGA, Kazutaka SHIMBO, Ayaka TOKUNAGA
  • Patent number: 8728772
    Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.
    Type: Grant
    Filed: December 7, 2012
    Date of Patent: May 20, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Shigeo Suzuki, Yoshihiro Usuda, Shuhei Hashiro
  • Patent number: 8354254
    Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.
    Type: Grant
    Filed: July 23, 2010
    Date of Patent: January 15, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Shigeo Suzuki, Yoshihiro Usuda, Shuhei Hashiro
  • Publication number: 20120315678
    Abstract: Glucose is produced by hydrolyzing starch contained in a microalga which belongs to the genus Desmodesmus and accumulates 30% or more of starch in algae bodies based on dry weight of the algae bodies when it is cultured under suitable conditions.
    Type: Application
    Filed: May 18, 2012
    Publication date: December 13, 2012
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Yoshihiro Usuda
  • Publication number: 20110014663
    Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.
    Type: Application
    Filed: July 23, 2010
    Publication date: January 20, 2011
    Inventors: Shigeo Suzuki, Yoshihiro Usuda, Shuhei Hashiro