Patents by Inventor Shuhei Hashiro
Shuhei Hashiro has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11884951Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.Type: GrantFiled: May 21, 2021Date of Patent: January 30, 2024Assignee: AJINOMOTO CO., INC.Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
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Publication number: 20210269840Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.Type: ApplicationFiled: May 21, 2021Publication date: September 2, 2021Applicant: AJINOMOTO CO., INC.Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
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Patent number: 11046986Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.Type: GrantFiled: September 25, 2019Date of Patent: June 29, 2021Assignee: AJINOMOTO CO., INC.Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
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Patent number: 10941390Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.Type: GrantFiled: June 10, 2020Date of Patent: March 9, 2021Assignee: AJINOMOTO CO., INC.Inventors: Noriko Yokoyama, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
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Publication number: 20210024931Abstract: A method for efficiently inducing RNA silencing in a target organism is provided. RNA silencing is induced in a target organism by allowing the target organism to ingest cells of a microorganism, wherein the microorganism is able to produce RNA that induces RNA silencing after treating the cells with an organic solvent.Type: ApplicationFiled: August 14, 2020Publication date: January 28, 2021Applicant: AJINOMOTO CO., INC.Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Nakano, Teruyuki Niimi, Haruka Kawaguchi
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Publication number: 20200318093Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.Type: ApplicationFiled: June 10, 2020Publication date: October 8, 2020Applicant: AJINOMOTO CO., INC.Inventors: Noriko YOKOYAMA, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
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Patent number: 10717971Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.Type: GrantFiled: August 31, 2016Date of Patent: July 21, 2020Assignee: AJINOMOTO CO., LTD.Inventors: Noriko Yokoyama, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
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Publication number: 20200024629Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.Type: ApplicationFiled: September 25, 2019Publication date: January 23, 2020Applicant: AJINOMOTO CO., INC.Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
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Publication number: 20170044513Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.Type: ApplicationFiled: August 31, 2016Publication date: February 16, 2017Applicant: AJINOMOTO CO., INC.Inventors: Noriko YOKOYAMA, Mayu NAKANO, Shuhei HASHIRO, Masako HIRAGA, Kazutaka SHIMBO, Ayaka TOKUNAGA
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Patent number: 8728772Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.Type: GrantFiled: December 7, 2012Date of Patent: May 20, 2014Assignee: Ajinomoto Co., Inc.Inventors: Shigeo Suzuki, Yoshihiro Usuda, Shuhei Hashiro
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Patent number: 8354254Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.Type: GrantFiled: July 23, 2010Date of Patent: January 15, 2013Assignee: Ajinomoto Co., Inc.Inventors: Shigeo Suzuki, Yoshihiro Usuda, Shuhei Hashiro
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Publication number: 20120315678Abstract: Glucose is produced by hydrolyzing starch contained in a microalga which belongs to the genus Desmodesmus and accumulates 30% or more of starch in algae bodies based on dry weight of the algae bodies when it is cultured under suitable conditions.Type: ApplicationFiled: May 18, 2012Publication date: December 13, 2012Applicant: AJINOMOTO CO., INC.Inventors: Shuhei Hashiro, Yoshihiro Usuda
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Publication number: 20110014663Abstract: An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.Type: ApplicationFiled: July 23, 2010Publication date: January 20, 2011Inventors: Shigeo Suzuki, Yoshihiro Usuda, Shuhei Hashiro