Patents by Inventor Shuhei Tsukamoto

Shuhei Tsukamoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of determining 1,5-anhydroglucitol, and reagent composition for determining 1,5-anhydroglucitol
    Patent number: 8945864
    Abstract: The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: February 3, 2015
    Assignees: Ikeda Food Research Co., Ltd., Nippon Kayaku Kabushiki Kaisha
    Inventors: Emi Ishimaru, Hirokazu Sanada, Hironori Omura, Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda
  • Thermostable 1,5-anhydroglucitol dehydrogenase, and method for measurement of 1,5-anhydroglucitol by using the same
    Patent number: 8574882
    Abstract: A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.
    Type: Grant
    Filed: June 18, 2009
    Date of Patent: November 5, 2013
    Assignee: Nippon Kayaku Kabushiki Kaisha
    Inventors: Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda, Reiko Machida, Yoshihiko Umegae, Masahiko Yabuuchi
  • THERMOSTABLE 1,5-ANHYDROGLUCITOL DEHYDROGENASE, AND METHOD FOR MEASUREMENT OF 1,5-ANHYDROGLUCITOL BY USING THE SAME
    Publication number: 20120094320
    Abstract: A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.
    Type: Application
    Filed: June 18, 2009
    Publication date: April 19, 2012
    Applicant: NIPPON KAYAKU KABUSHIKI KAISHA
    Inventors: Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda, Reiko Machida, Yoshihiko Umegae, Masahiko Yabuuchi