Abstract: Firefly luciferase gene for making use of the luminescence reaction of luciferase is provided.

Abstract: Nucleic acids coding for the flavin reductase of a luminescent bacterium, Xenorhabdus luminescens, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing substantially purified recombinant flavin reductase, and recombinantly produced flavin reductase and equivalent proteins produced in accordance with such methods are provided.

Abstract: A flavin reductase gene is provided having the nucleotide sequence of SEQ ID NO:1, wherein the sequence is displayed in numbered triplets of capital letters, which numbers proceed sequentially from left to right and from the 5' terminus to the 3' terminus; the sequence of capital letters represent the purine and pyrimidine bases of the nucleotide sequence, as follows:A is adenine; G is guanine; C is cytosine; T is thymine;R is A or G; Y is T or C; N is A, T, C, or G; H is A, C, or T; and M is A or C;wherein triplet number 237 thereof is TAA or TAG or TGA; and wherein further:for triplets numbered 13, 21, 22, 36, 63, 85, 92, 111, 112, 126, 140, 150, 156, 186, 196, and 228, if the 3' nucleotide of a triplet is A or G, then the 5' nucleotide of the triplet is T or C, or if the 3' nucleotide of a triplet is T or C, then the 5' nucleotide of the triplet is C; and if the 5' nucleotide of a triplet is C, then the 3' nucleotide of the triplet is A, T, C, or G, or if the 5' nucleotide of a triplet is T, then the 3' nu

Abstract: The present invention succeeds in isolating a gene encoding an enzyme having an FMN reducing activity and a nitroreductase activity derived from luminous bacteria Vibrio fischeri (ATCC 7744), elucidating its primary structure, and producing Escherichia coli which can express the gene in large quantities. That is, the present invention provides a gene encoding an enzyme having the flavin reducing activity and the nitro-reductase activity, an enzyme produced therefrom, a recombinant vector containing the gene, and bacteria containing the recombinant vector.

Abstract: A process for secretory production of a calcium-binding protein very useful for producing heterogenic proteins in Escherichia coli according to gene recombinant technique is provided, which process comprises using Escherichia coli and using a secretory expression system with the promoter of lipoprotein and the gene of signal peptide of outer membrane protein A, according to recombinant DNA technique.