Abstract: A quantitative analysis method based on air pressure measuring, which can be used for the high-sensitivity quantitative detection of various targets i.e. inorganic ions, small molecules and biological macromolecules such as proteins, DNA, and even viruses, bacteria, cells, etc. The present invention catalyzes hydrogen peroxide to generate a large amount of gas using enzymes or nanoparticles, etc.; converts the target molecule detection signal into a gas pressure intensity signal; achieves signal amplification; and finally converts the pressure change into an electrical signal to conduct a reading through an air pressure meter, thereby achieving high-sensitivity quantitative detection. The feasibility, wide applicability and reliability of the present invention are certified through three different detection systems, i.e. an ELISA system, a DNA hydrogel system and a functional DNA sensor system, respectively, using an air pressure meter.