Abstract: The present invention relates to a transformant which uses Schizosaccharomyces pombe as a host into which a D-LDH gene derived from bacteria of the genus Pediococcus and a D-LDH gene derived from bacteria of the genus Lactobacillus are incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated.

Abstract: There is provided a glass substrate for observing minute substance, made of porous glass and capable of separating and capturing a minute substance with a 10 to 500 nm average particle diameter contained in a solution or a suspension, comprising a porous glass substrate having a plurality of pores, wherein the plurality of pores has an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance, each of the plurality of pores has a surface pore diameter on an uppermost surface of the glass substrate, a standard deviation of the surface pore diameter is 60% or less of the average particle diameter of the minute substance, and a pore with a pore diameter ranging from 60 to 140% of a pore diameter at peak top in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume.

Abstract: The present invention relates to a transformant which uses Schizosaccharomyces pombe as a host into which a D-LDH gene derived from bacteria of the genus Pediococcus and a D-LDH gene derived from bacteria of the genus Lactobacillus are incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated.

Abstract: Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant comprising 3 copies of a human lactate dehydrogenase gene that is introduced into a Schizosaccharomyces pombe host, wherein a gene encoding pyruvate decarboxylase 2 of the Schizosaccharomyces pombe host is deleted or inactivated.

Abstract: Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant containing from 3 to 5 copies of a lactate dehydrogenase gene derived from human and introduced into a Schizosaccharomyces pombe host, wherein a gene that is a part of a group of genes encoding pyruvate decarboxylase of the Schizosaccharomyces pombe host is deleted or inactivated. A transformant characterized by providing a cell concentration of at least 4.0 g (on a dry cell weight basis)/L of a culture broth prepared by inoculating cells, at an initial cell concentration of 0.

Abstract: There is provided a glass substrate for observing minute substance, made of porous glass and capable of separating and capturing a minute substance with a 10 to 500 nm average particle diameter contained in a solution or a suspension, comprising a porous glass substrate having a plurality of pores, wherein the plurality of pores has an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance, each of the plurality of pores has a surface pore diameter on an uppermost surface of the glass substrate, a standard deviation of the surface pore diameter is 60% or less of the average particle diameter of the minute substance, and a pore with a pore diameter ranging from 60 to 140% of a pore diameter at peak top in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume.

Abstract: Provided is a transformant of S. pombe mutant which can produce and collect ?-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus Schizosaccharomyces. The transformant of a S. pombe mutant of the present invention is a transformant of a Schizosaccharomyces pombe mutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a ?-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus Schizosaccharomyces, and is useful for transforming a yeast of the genus Schizosaccharomyces, an expression vector, a transformant, etc.

Abstract: Provided is a transformant which uses Schizosaccharomyces pombe as a host, into which one or more foreign genes selected from the group consisting of a phosphoenolpyruvate carboxykinase gene and a pyruvate carboxylase gene are incorporated, and in which pdc2 genes of the Schizosaccharomyces pombe host have undergone deletion or deactivation, and a method for manufacturing a dicarboxylic acid having 4 carbon atoms by using the transformant.

Abstract: Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Abstract: Provided is a transformant of S. pombe mutant which can produce and collect ?-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus Schizosaccharomyces. The transformant of a S. pombe mutant of the present invention is a transformant of a Schizosaccharomyces pombe mutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a ?-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus Schizosaccharomyces, and is useful for transforming a yeast of the genus Schizosaccharomyces, an expression vector, a transformant, etc.

Abstract: Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.