Abstract: Disclosed is a fluorescent compound for the detection of isocyanate substances, a preparation method therefor and use thereof as a test-paper-type detection probe. The fluorescent compound is 2,4-di(((4?-(diphenylamino)-[1,1?-biphenyl]-4-yl)imino)methyl)phenol. The fluorescent compound is prepared by means of a one-step method. The fluorescent compound has simple and convenient preparation with high yield, and is capable of making a rapid and specific response to isocyanate substances. Moreover, the fluorescence intensity of the fluorescent compound will enhance with the increase of the isocyanate concentration. The fluorescent compound can be made into a portable test-paper-type probe for the detection of isocyanate substances in air, and can achieve the visual detection of volatile isocyanate gases. The probe has an aggregation-induced emission effect, and thus it has higher fluorescence quantum yield when using a test-paper-type probe for detection.

Abstract: Disclosed is a fluorescent compound for the detection of isocyanate substances, a preparation method therefor and use thereof as a test-paper-type detection probe. The fluorescent compound is 2,4-di(((4?-(diphenylamino)-[1,1?-biphenyl]-4-yl)imino)methyl)phenol. The fluorescent compound is prepared by means of a one-step method. The fluorescent compound has simple and convenient preparation with high yield, and is capable of making a rapid and specific response to isocyanate substances. Moreover, the fluorescence intensity of the fluorescent compound will enhance with the increase of the isocyanate concentration. The fluorescent compound can be made into a portable test-paper-type probe for the detection of isocyanate substances in air, and can achieve the visual detection of volatile isocyanate gases. The probe has an aggregation-induced emission effect, and thus it has higher fluorescence quantum yield when using a test-paper-type probe for detection.

Abstract: The present invention relates to a fluorescent probe for detecting nitroreductase and a preparation method and use thereof in enzymatic reactions, belonging to the field of industrial analysis and detection. The fluorescent probe is 3-(4-(2-(4?-(diphenylamino)-3-((4-nitrobenzyl)oxy)-[1, 1?-biphenyl]-4-yl)vinyl)quinolin-1-ium-1-yl)propane-1-sulfonate. The fluorescent probe of the present invention, with the introduction of hydrophilic groups, sulfonate and quinolinium, the probe's hydrophilicity is enhanced, under the enzymatic catalysis of nitroreductase (NTR), 1,6-rearrangement and elimination reaction occurs, and hydroxyl group is generated. Detection and analysis of the NTR in the industrial enzymatic reactions can be realized due to the change of fluorescence which is induced by the intramolecular charge transfer (ICT) effect.

Abstract: The present invention relates to a fluorescent probe for detecting nitroreductase and a preparation method and use thereof in enzymatic reactions, belonging to the field of industrial analysis and detection. The fluorescent probe is 3-(4-(2-(4?-(diphenylamino)-3-((4-nitrobenzyl)oxy)-[1,1?-biphenyl]-4-yl)vinyl)quinolin-1-ium-1-yl)propane-1-sulfonate. The fluorescent probe of the present invention, with the introduction of hydrophilic groups, sulfonate and quinolinium, the probe's hydrophilicity is enhanced, under the enzymatic catalysis of nitroreductase (NTR), 1,6-rearrangement and elimination reaction occurs, and hydroxyl group is generated. Detection and analysis of the NTR in the industrial enzymatic reactions can be realized due to the change of fluorescence which is induced by the intramolecular charge transfer (ICT) effect.