Abstract: It is an object of the present invention to provide: a method for preparing a cell concentrate which efficiently separates nucleated cells from unnecessary cells contained in a cell-containing solution by simple operations, thereby reducing the volume of a solution used for cryopreservation that contains the nucleated cells, when the cell-containing solution that contains the nucleated cells and the unnecessary cells has been filtrated with a filter device and when a recovery solution is then introduced into the filter device to recover the nucleated cells captured by a filter material; and a cell composition.

Abstract: A method for effectively recovering nucleated cells from cell-containing fluid containing nucleated cells and cells to be removed by introducing cell-containing fluid into a filter and recovering the nucleated cells captured in the filter, recovering liquid dispersing element is used in combination with nucleated cells capturing elements, to constitute specific arrangement, and further a comparatively flat filter, where an effective filtering membrane area and packing thickness are in a specific ratio, is used.

Abstract: A method for regenerating a blood vessel comprising introducing a cell-containing fluid containing vascular endothelial precursor cells and cells to be removed into a cell separator which allows at least the cells to be removed to substantially pass through but substantially captures the vascular endothelial precursor cells; recovering the vascular endothelial precursor cells once captured on said cell separator by introducing a fluid into the said cell separator;

Abstract: A method of regenerating a tissue comprising the steps of collecting a cell-containing fluid which contains tissue-regenerating cells for use in regeneration of the tissue and cells to be removed; introducing the collected cell-containing fluid into a cell-capturing means capable of at least capturing the cell-regenerating cells and permitting passage therethrough of the cells to be removed; then introducing a liquid into said cell-capturing means to recover therefrom the cell-regenerating cells which have been captured by said cell-capturing means; and further using the recovered tissue-regenerating cells for regeneration of the tissue.

Abstract: A cell separation method comprising steps of introducing a cell-containing fluid containing cells to be recovered and cells to be removed, into a cell-capturing means capable of substantially capturing the cells to be recovered and substantially permitting passage therethrough of cells to be removed; taking out the resulting fluid containing the cells to be removed, from the cell-capturing means; and then introducing a liquid with a viscosity of not more than 500 mPa·s and not less than 5 mPa·s into the cell-capturing means to recover therefrom the cells to be recovered which have been captured by the cell-capturing means.

Abstract: A cell separation method comprising steps of introducing a cell-containing fluid containing cells to be recovered and cells to be removed, into a cell-capturing means capable of substantially capturing the cells to be recovered and substantially permitting passage therethrough of cells to be removed; taking out the resulting fluid containing the cells to be removed, from the cell-capturing means; and then introducing a liquid with a viscosity of not more than 500 mPa·s and not less than 5 mPa·s into the cell-capturing means to recover therefrom the cells to be recovered which have been captured by the cell-capturing means.