Abstract: Provided herein are compositions, methods, and systems for sample processing and/or data analysis. Sample processing may include nucleic acid sample processing and subsequent sequencing. Methods and systems of the present disclosure can be used, for example, for the analysis of a nucleic acid sample from a human, non-human, and combinations thereof.

Abstract: The present invention provides a bispecific antibody and use thereof, and the bispecific antibody or an antigen-binding fragment of the present invention can bind to two or more antigens or two or more epitopes of a same antigen. The antibody or the antigen-binding fragment of the present invention can be used to treat a variety of diseases, such as inflammatory diseases, autoimmune diseases, cancers or spinal cord injury, and can also be used for diagnosis and prognosis of related diseases.

Abstract: The invention provides a retractable friction stir welding spindle, aims at the deficiencies and defects of the existing friction stir welding spindle technology, that is, when the welding is finished, the stirring needle leaves a keyhole at the end of the weld seam, which affects the forming performance of the workpiece, the overall structure design of the spindle is complicated with many transmission chains and low reliability which is difficult to realize modularization. The retractable friction stir welding spindle includes an electric spindle system and a retracting mechanism. The electric spindle system includes stirring shaft shoulder, hilt, front end cover, hollow spindle, motorized spindle shell, motor rotor, motor stator, tail end cover, etc. The retracting mechanism includes stirring needle, connecting ball head and electric cylinder. The invention has the advantages of simple structure and can be mainly divided into two modules to improve reliability and integrity.

Abstract: A device and a method for controlling a size of a molten pool in a wire and arc additive manufacturing process are provided. The device includes additive manufacturing equipment, a connecting device, bilateral gas flow devices and a CCD camera. By adjusting the connecting device, the bilateral gas flow devices and a welding gun have proper relative positions. The CCD camera is clamped on a rear side of the welding gun and matched with a proper optical filter to detect size information of the molten pool. In the additive manufacturing process, the bilateral gas flow devices and the welding gun keep moving synchronously, welding wires are conveyed to a designed position of a deposited layer by a wire feeding device, and bilateral gas flows can directly and synchronously act on a melting region. A flow controller is adjusted in real time according to the size of the molten pool.

Abstract: The invention provides a retractable friction stir welding spindle, aims at the deficiencies and defects of the existing friction stir welding spindle technology, that is, when the welding is finished, the stirring needle leaves a keyhole at the end of the weld seam, which affects the forming performance of the workpiece, the overall structure design of the spindle is complicated with many transmission chains and low reliability which is difficult to realize modularization. The retractable friction stir welding spindle includes an electric spindle system and a retracting mechanism. The electric spindle system includes stirring shaft shoulder, hilt, front end cover, hollow spindle, motorized spindle shell, motor rotor, motor stator, tail end cover, etc. The retracting mechanism includes stirring needle, connecting ball head and electric cylinder. The invention has the advantages of simple structure and can be mainly divided into two modules to improve reliability and integrity.

Abstract: A train compartment brake control method includes: acquiring the number of train compartments of a current train; acquiring the number and type of a current train compartment; and on the basis of a train brake instruction and the number of train compartments of the current train, calculating a braking force of the current train compartment, and performing brake control on the current train compartment. The technical solution described in the present application is applicable to a train having any number of train compartments. The above method acquires the number of train compartments of a train in real time, calculates the braking force required by each train compartment according to the number and type of a current train compartment, and performs brake control on the train.

Abstract: A method for train suspension control by means of multiple air springs includes: receiving a vehicle load pressure; and controlling height adjustment valves according to the vehicle load pressure to adjust the pressure of a first air spring set, a second air spring set and/or a third air spring set. Three height adjustment valves are provided, and the three height adjustment valves form a triangular structure. Each of the first air spring set, the second air spring set and the third air spring set comprises a plurality of individual air springs, and all the individual air springs in each of the air spring sets are correspondingly connected to the same height adjustment valve. A system for train suspension control by means of multiple air springs and a train are further provided.

Abstract: A train compartment brake control method comprises the following steps: acquiring the number of train compartments of a current train; acquiring the number and type of a current train compartment; and on the basis of a train brake instruction and the number of train compartments of the current train, calculating a braking force of the current train compartment, and performing brake control on the current train compartment. The technical solution described in the present application is applicable to a train having any number of train compartments. The above method acquires the number of train compartments of a train in real time, calculates the braking force required by each train compartment according to the number and type of a current train compartment, and performs brake control on the train.

Abstract: A low-back-pressure penetrating arc welding apparatus and a welding method using the same are disclosed. Through vacuuming a central vacuum chamber of a low-back-pressure protection device by a suction device, a plume of a penetrating arc located in the central vacuum chamber, as well as a back side of a keyhole molten pool, are maintained in a negative-pressure vacuum state relative to an argon gas ambience. A pressure gradient in the keyhole molten pool is thus generated that points from a front side of the keyhole molten pool to the back side of the keyhole molten pool. The pressure gradient thus further enhances a stiffness of the front side of the keyhole molten pool as well as a stability of the penetrating arc, resulting in an enhancement in the piercing capability of the penetrating arc, without changing a force distribution and a temperature gradient of the keyhole molten pool.

Abstract: Gas metal arc welding (GMAW) is a widely used process for joining metals. Its main advantage over its competition gas tungsten arc welding (GTAW) is its high productivity in depositing metals. However, to melt metal from the wire to deposit into the work-piece, additional heat is consumed and applied to the work-piece with an uncontrolled fixed proportion to the effective heat that melts the wire. Such additional heat is often in excess of the needed heat input for the work-piece. The side-effects include a waste of the energy, an increased distortion, and possible materials property degradation. This invention is to device a method to transfer this part of heat to melt the wire by adding two wires, which form a pair of arc spots, under a tungsten arc. It also devices a method to assure the arc between the two wires be maintained stable such that the transfer be successfully continuous.

Abstract: A low-back-pressure penetrating arc welding apparatus and a welding method using the same are disclosed. Through vacuuming a central vacuum chamber of a low-back-pressure protection device by a suction device, a plume of a penetrating arc located in the central vacuum chamber, as well as a back side of a keyhole molten pool, are maintained in a negative-pressure vacuum state relative to an argon gas ambience. A pressure gradient in the keyhole molten pool is thus generated that points from a front side of the keyhole molten pool to the back side of the keyhole molten pool. The pressure gradient thus further enhances a stiffness of the front side of the keyhole molten pool as well as a stability of the penetrating arc, resulting in an enhancement in the piercing capability of the penetrating arc, without changing a force distribution and a temperature gradient of the keyhole molten pool.

Abstract: The present disclosure relates to a central negative pressure arc welding apparatus and method. Through a pumping gas pipeline from the suction device to hollow tubular electrode, a suction device may establish a stable, high energy density and binding arc beneath hollow tubular electrode. The binding degree, energy density and voltage of arc may be adjusted by changing the value of negative pressure in the center of arc. Accordingly, welding heat input and coefficient of weld may be precisely controlled. This apparatus may further set up central pulsating negative pressure arc by turning on valve periodically to make a periodic negative pressure in the center of arc. This present disclosure both possesses good stability and adaptability of traditional Gas Tungsten Arc Welding and achieves binding arc and good penetration of Plasma Arc Welding. Furthermore, this present disclosure improves the mechanical properties of welded joints.

Abstract: Gas metal arc welding (GMAW) is a widely used process for joining metals. Its main advantage over its competition gas tungsten arc welding (GTAW) is its high productivity in depositing metals. However, to melt metal from the wire to deposit into the work-piece, additional heat is consumed and applied to the work-piece with an uncontrolled fixed proportion to the effective heat that melts the wire. Such additional heat is often in excess of the needed heat input for the work-piece. The side-effects include a waste of the energy, an increased distortion, and possible materials property degradation. This invention is to device a method to transfer this part of heat to melt the wire by adding two wires, which form a pair of arc spots, under a tungsten arc. It also devices a method to assure the arc between the two wires be maintained stable such that the transfer be successfully continuous.

Abstract: A vacuum pump comprises an actuator with an actuating shaft; a mounting base with a first side being attached to the actuator; a pumping device having a pump ring being attached to a second side of the mounting base opposite to the first side; and a sound-proof cap being attached to an end face of the pump ring and the pumping device being driven by the actuating shaft penetrating through the mounting base.