Patents by Inventor Shuling NIE

Shuling NIE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240052308
    Abstract: A preparation method of a human scalp hair follicle single cell suspension includes the following steps: step 1, a fresh isolated scalp tissue is pretreated with a digestive enzyme solution in a low temperature environment; step 2, the hair follicles can be effectively dissociated by digestion with the digestive enzyme solution at 30-37° C.; step 3, the dissociated hair follicles are digested with pancreatin-EDTA to obtain a human scalp hair follicles single cell suspension. In addition, the invention discloses a human scalp hair follicle single cell suspension prepared by the above method and use thereof. The method of the present invention is short in time and the hair follicles dissociated are high in integrity, and the subsequent isolated single cell populations are large in number and high in vitality, which solves the problems of incomplete separation of hair follicles from isolated scalp tissue, long cell extraction time, and low cell viability.
    Type: Application
    Filed: May 7, 2021
    Publication date: February 15, 2024
    Applicant: SHANGHAI ICURE BIOSCIENCE CO., LTD
    Inventors: Yamei SUN, Wen GU, Shaohua MA, Shuling NIE